1. WILLIAM J. FRAZEE, Ph.D.
1114 Minton Road
Madison, Wisconsin 53711-3141
608-442-7101 (home)
608-669-8055 (cell)
frazeewj@gmail.com
PATENTS
Zelle, R.; Galullo, V. P.; Baker, C. T.; Will, P.; Frazee, W. J. Ion Channel Modulators. WO 2005/087750 A1
(published 22 September 2005).
Zelle, R.; Galullo, V. P.; Baker, C. T.; Will, P.; Frazee, W. J.; Masdiyasni, H.; Guo, J. Ion Channel Modulators.
WO 2005/086836 A2 (published 22 September 2005).
Zelle, R.; Galullo, V. P.; Baker, C. T.; Will, P.; Frazee, W. J.; Mazdiyasni, H.; Guo, J. Ion Channel Blockers for
Treating Disease. PCT Application No. PCT/US05/07667 (filed 7 March 2005).
Jacobs, R. T.; Folmer, J. J.; Simpson, T. R.; Chaudhari, B.; Frazee, W. J.; Davenport, T. W.; Sundarababu, G.
Therapeutic Heterocycles. US Patent 6,399,603 (4 June 2002).
Lewis, J. J.; Brush, K. A.; Garcia-Davenport, L. E.; Frazee, W. J.; Chapdelaine, M. J. Azolobenzazepine Derivatives
and Compositions and Method of Use Thereof. US Patent 6,313,290 (6 November 2001).
Jacobs, R. T.; Folmer, J.; Simpson, T. R.; Chaudhari, B.; Frazee, W. J.; Davenport, T. W. Therapeutic Quinazoline
Compounds. WO 01/21598 (published 29 March 2001).
Brush, K. A.; Chapdelaine, M. J.; Frazee, W. J.; Garcia-Davenport, L. E.; Lewis, J. J. Azolobenzazepine Derivatives
as Neurologically Active Agents. WO 97/32883 (published 12 September 1997), US Patent 6,124,281 (26 September
2000).
PUBLICATIONS
Marks, B. D.; Fakhoury, S. A.; Frazee, W. J.; Eliason, H. C.; Riddle, S. M. A Substrate-Independent TR-FRET
Histone Deacetylase Inhibitor Assay. J. Biomol. Screen. 2011, 16, 1247-1253.
Lebakken, C. S.; Riddle, S. M.; Singh, U.; Frazee, W. J.; Eliason, H. C.; Gao, Y.; Reichling, L. J.; Marks, B. D.;
Vogel, K. W. Development and Applications of a Broad-Coverage, TR-FRET Based Kinase Binding Assay Platform.
J. Biomol. Screen. 2009, 14, 924-935.
Shukla, S. J.; Nguyen, D.-T.; MacArthur, R.; Simeonov, A.; Frazee, W. J.; Hallis, T. M.; Marks, B. D.; Singh, U.;
Eliason, H. C.; Printen, J.; Austin, C. P.; Inglese, J.; Auld, D. S. Identification of Pregnane X Receptor Ligands Using
Time-Resolved Fluorescence Resonance Energy Transfer and Quantitative High-Throughput Screening. Assay Drug
Dev. Technol. 2009, 7, 143-169.
Piper, D. R.; Duff, S. R.; Eliason, H. C.; Frazee, W. J.; Frey, E. A.; Fuerstenau-Sharp, M.; Jachec, C.; Marks, B. D.;
Pollok, B. A.; Shekhani, M. S.; Thompson, D. V.; Whitney, P.; Vogel, K. W.; Hess, S. D. Development of the
Predictor hERG Fluorescence Polarization Assay Using a Membrane Protein Enrichment Approach. Assay Drug Dev.
Technol. 2008, 6, 213-223.

2. WILLIAM J. FRAZEE, Ph.D.
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Scott, C. W.; Sobotka-Briner, C.; Wilkins, D. E.; Jacobs, R. T.; Folmer, J. J.; Frazee, W. J.; Bhat, R. V.; Ghanekar,
S. V.; Aharony, D. Novel Small Molecule Inhibitors of Caspase-3 Block Cellular and Biochemical Features of
Apoptosis. J. Pharmacol. Exp. Therap. 2003, 304, 433.
Frazee, W. J. Dimethyl Bromomalonate. In Encyclopedia of Reagents for Organic Synthesis; Paquette, L. A., Ed.;
Wiley: New York, 1995; Vol.3, pp 2036-2038.
Frazee, W. J.; Ohnmacht, C. J.; Malick; J. B. Antidepressants. Annual Reports in Medicinal Chemistry 1985, 20, 31.
Ko, K.-Y.; Frazee, W. J.; Eliel, E. L. Asymmetric Reactions Based on 1,3-Oxathianes—3. Secondary α-Hydroxyacids
RCHOHCO2
H and Glycols RCHOHCH2OH. Tetrahedron 1984, 40, 1333.
Ohnmacht, C. J.; Malick, J. B.; Frazee, W. J. Antidepressants. Annual Reports in Medicinal Chemistry 1983, 18, 41.
Eliel, E. L.; Koskimies, J. K.; Lohri, B.; Frazee, W. J.; Morris-Natschke, S.; Lynch, J. E.; Soai, K. Asymmetric
Synthesis of Chiral Tertiary Alcohols in High Enantiomeric Excess; in Asymmetric Reactions and Processes in
Chemistry, Eliel, E. L.; Otsuka, S., Eds.; American Chemical Society, Washington, DC, 1982, p. 37.
Posner, G. H.; Babiak, K. A.; Loomis, G. L.; Frazee, W. J.; Mittal, R. D.; Karle, I. L. Stereocontrolled Total Synthesis
of an α-Methylene Guaianolide in the 4,5-Epoxyosmitopsin Family. J. Am. Chem. Soc. 1980, 102, 7498.
Eliel, E. L.; Frazee, W. J. Asymmetric Synthesis of Nearly Optically Pure Atrolactic Acid Methyl Ether. J. Org.
Chem. 1979, 44, 3598.
Posner, G. H.; Loomis, G. L.; Mittal, R. D.; Frazee, W. J.; Schmidt, D. M.; Babiak, K. A. Synthesis and
Characterization of Two Stereoisomeric Hydroazulenones. Versatile Precursors to Guaianolides. Tetrahedron Lett.
1978, 4213.
Trost, B. M.; Bogdanowicz, M. J.; Frazee, W. J.; Salzmann, T. N. Oxasecoalkylation via Cyclobutanone Intermediates.
J. Am. Chem. Soc. 1978, 100, 5512.
Trost, B. M.; Frazee, W. J. New Synthetic Reactions. Double Chain Extension. J. Am. Chem. Soc. 1977, 99, 6124.
Allen, J. R.; Chesney, C. F.; Frazee, W. J. Modifications of Pyrrolizidine Alkaloid Intoxication Resulting from Altered
Hepatic Microsomal Enzymes. Toxicol. Appl. Pharmacol. 1972, 23, 470.
Kretchmer, R. A.; Frazee, W. J. Hydroazulenes. I. A Thermal Epoxide Rearrangement. J. Org. Chem. 1971, 36, 2855.
POSTERS & PRESENTATIONS
Frazee, W. J.; Riddle, S. M.; Wolken, J. K.; Marks, B. D.; Lebakken, C. S.; Eliason, H. E.; Singh, U.; Rukavishnikov,
A.; Fakhoury, S. A. Competitive TR-FRET Binding Assays for PI3K Family Inhibitors. Society for Biomolecular
Sciences 16th
Annual Conference, Phoenix, AZ, 2010.
Singh, U.; Lebakken, C.; Riddle, S.; Marks, B.; Frazee, J.; Eliason, H.; Wolken, J.; Gao, Y.; Reichling, L.; Vogel, K.
New fluorescence-based binding assay for identification and characterization of kinase inhibitors. Abstracts of Papers,
237th
National Meeting of the American Chemical Society, Salt Lake City, Utah; American Chemical Society;
Washington, DC, 2008; MEDI 225.

3. WILLIAM J. FRAZEE, Ph.D.
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Piper, D. R.; Frazee, W. J.; Marks, B. D.; Hess, S. D. The Predictor™ Fluorescence Polarization Assay for High-
Throughput Screening of hERG Channel Affinity. Abstracts of Papers, 31st
National Medicinal Chemistry
Symposium, Pittsburgh, Pennsylvania; Division of Medicinal Chemistry, American Chemical Society, Washington,
DC, 2008; Abstract 242.
Riddle, S.; Singh, U.; Lebakken, C.; Frazee, J.; Marks, B.; Eliason, H.; Wolken, J.; Vogel, K. A One-step, Mix-and-
Read, TR-FRET Binding Assay to Characterize Kinase Inhibitors. 3rd
Protein Kinases in Drug Discovery Conference,
San Diego, CA, 2008.
Hereley, S.; Singh, U.; Lebakken, C.; Frazee, J.; Marks, B.; Eliason, H.; Wolken, J.; Riddle, S.; Vogel, K. A One-step,
Mix-and-Read, TR-FRET Binding Assay to Characterize Kinase Inhibitors. Society for Biomolecular Sciences 14th
Annual Conference, St. Louis, MO, 2008.
Piper, D. R.; Duff, S. R.; Eliason, H. C.; Frazee, W. J.; Frey, E. A.; Fuerstenau-Sharp, M.; Jachec, C.; Marks, B. D.;
Pollok, B. A.; Shekhani, M. S.; Thompson, D. V.; Whitney, P.; Vogel, K. W.; Zhong, Z.; Hess, S. D. Building the
Predictor™ Fluorescence Polarization Assay for High-Throughput Screening of hERG Channel Affinity. Society for
Biomolecular Sciences 14th
Annual Conference, St. Louis, MO, 2008.
VanDenHeuvel-Kramer, K.; Marks, B. D.; Stafslien, D. K.; Singh, U.; Eliason, H. C.; Wolken, J. K.; Frazee, W. J.
Fluorescence-based Biochemical Assays for the Study of Pregnane X Receptor and Constitutive Androstane Receptor.
5th
Annual Screening Europe Conference, Stockholm, Sweden, 2008.
Leathers, R.; Kupcho, K.; Stafslien, D. K.; Singh, U.; Eliason, H. C.; Reichling, L.; Frazee, W. J.; Horton, R. A.;
Riddle, S.; Gao, Y. Enabling compound profiling across the entire PI3K family using the Adapta™ universal kinase
assay. 5th
Annual Screening Europe Conference, Stockholm, Sweden, 2008.
Frazee, W. J.; Singh, U.; Marks, B. D.; Eliason, H. E.; Stafslien, D. K.; Wolken, J. K.; Vogel, K. W. Early Screening
for PXR- and CAR-mediated ADME-Tox liabilities: New fluorescence-based ligand binding and coregulator
recruitment assays. Abstracts of Papers, 235th
National Meeting of the American Chemical Society, New Orleans,
Louisiana; American Chemical Society; Washington, DC, 2008; MEDI 059.
Singh, U.; Marks, B. D.; Eliason, H. E.; Stafslien, D. K.; Wilkinson, J. M.; De Rosier, T.; Hallis, T. M.; Vogel, K. W.;
Miao, G.; Frazee, W. J. New fluorescence-based assays for identification and characterization of selective PPARα,
δ(β), and γ ligands. Abstracts of Papers, 235th
National Meeting of the American Chemical Society, New Orleans,
Louisiana; American Chemical Society; Washington, DC, 2008; MEDI 061.
Hereley, S.; Marks, Bryan D.; Stafslien, D. K.; Singh, U.; Eliason, H. C.; Wolken, J. K.; Frazee, W. J. Fluorescence-
Based Biochemical Assays for the Study of Pregnane X Receptor and Constitutive Androstane Receptor. 8th
International ISSX (International Society for the Study of Xenobiotics) Meeting, Sendai, Japan, October 9-12, 2007.
Duff, S. R.; Farnham, J. G.; Marks, B. D.; Qadir, N.; Eliason, H. C.; Shekhani, M. S.; Fernandez-Perez, C.; Vogel, K.
W.; Frazee, W. J. Fluorescence polarization (FP) assay for identification of vitamin D receptor (VDR) ligands.
Abstracts of Papers, 232nd
National Meeting of the American Chemical Society, San Francisco, California; American
Chemical Society; Washington, DC, 2006; MEDI 543.
Brush, K. A.; Frazee, W. J.; Garcia-Davenport, L. E.; Lewis, J. J.; Goldstein, J. M.; Pullan, L. M. Synthesis and
Structure Activity Studies of a Novel Series of Glycine Antagonists: Tricyclic Benzazepine Analogs. Abstracts of
Papers, 213th
National Meeting of the American Chemical Society, San Francisco, California; American Chemical
Society; Washington, DC, 1997; MEDI 34.

4. WILLIAM J. FRAZEE, Ph.D.
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Payne-Gallimore, P. A.; Frazee, W. J.; Brush, K. A.; Pullan, L. M.; Goldstein, J. M.; Patel, J. B. Synthesis and
Structure Activity Studies of a Novel Series of Tricyclic Benzazepine Analogs. Abstracts of Papers, 213th
National
Meeting of the American Chemical Society, San Francisco, California; American Chemical Society; Washington, DC,
1997; MEDI 35.
Silverman, S. M.; Frazee, W. J.; Hall, J. E.; Ceccarelli, C.; Chow, M. M. A Method for Determination of the Oxime
Stereochemistry of Isatin-3-oximes Utilizing 13
C NMR Spectroscopy. Abstracts of Papers, 203rd
National Meeting of
the American Chemical Society, San Francisco, California; American Chemical Society; Washington, DC, 1992;
ORGN 329.
Frazee, W. J.; Chesney, C. F.; Allen, J. R. Effects of Altered Hepatic Drug-Metabolizing Enzyme Activities on the
Antimitotic Action of Monocrotaline in the Liver of Rats. Federation Proc. 1972, 31, 611.