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S
Tomomi Shore, Shawn Cyran
Biomedicine Program, Academies@Englewood
March 5, 2015
What is a planarian?• Classified in the
Platyhelminthes
phylum or “flat worms”
• Carnivores
• No anus and eats
using pharynx in
middle of its body
• Has central nervous
system
Flat worm with ability to regenerate
What is a planarian?
Continued
• Sexually reproduce and asexually reproduce through
division
• Hypotheses for regeneration:
• Epimorphosis- neoblasts
• Morphallaxis- morphogens give instructions
• Polar Coordinate model- molecules act as “zip code”
S Retinoids (found in animals) and carotenoids (found
in plants) are two subgroups of vitamin A group.
S Retinoids provide immune, inflammatory, genetic,
and reproductive benefits and are important to cell
growth and embryonic development.
Retinol
Purpose/Hypothesis
S Since Vitamin A is essential to growth and
maintenance of the body, we studied the effects of
foods with varying amounts of vitamin A on
Planarian regeneration.
S We hypothesized that Planarians fed greater
amounts of vitamin A would show greater
regeneration compared to others fed less.
Vitamin A Retinoid Found in
Foods
Food Vitamin A per 100g
Chicken Breast 50 IU
Egg Yolk 1,442 IU
Beef Liver 53,400 IU
Turkey Liver 72,350 IU
S We predicted that turkey liver would have the greatest
growth while chicken breast would have the least
because turkey liver has the most vitamin A, and chicken
breast has the least.
Methods
Feed
planarians
designated
food one
week
before
amputation
Place
(using
pipette)
Planarian
on ice
mount for
40-80
seconds
Put
Planarian
on coffee
filter on
petri dish
to
surgically
amputate
the head
Remove
coffee filter,
place
graph
paper
under petri
dish
Before After
Head
Body
Pictures
Results: Head Pieces Growth
Food Day 0 Day 1 Day 5 Day 6 Day 12
Turkey
Liver
0 0.15833
3333
1.43333
3333
1.65 1.93333
333
Beef
Liver
0 0.11666
6667
0.66666
6667
0.76666
6667
1.38666
6667
Egg Yolk 0 0 0.73333
333
0.93333
333
1.33333
33
Chicken
Breast
0 0.25333
3333
1 1.01666
6667
1.2
Food Day 0 Day 1 Day 5 Day 6
Turkey
Liver
0 0.116666
667
1.916666
667
2.3
Beef
Liver
0 0.063333
333
1.283333
33
1.283333
33
Egg Yolk 0 0.173333
33
0.84 1.79
Chicken
Breast
0 0.1 0.716666
66
1.12
Results: Body Pieces Growth
Graphs: Heads Growth
0
0.5
1
1.5
2
2.5
Day 0 Day 1 Day 5 Day 6 Day 12
Turkey Liver
Beef Liver
Egg Yolk
Chicken Breast
Graphs: Bodies Growth
0
0.5
1
1.5
2
2.5
Day 0 Day 1 Day 5 Day 6
Turkey Liver
Beef Liver
Egg Yolk
Chicken Breast
Analysis
S In both the heads and bodies, the turkey liver groups
had the greatest total growth compared to the
others.
S In both the heads and bodies, the chicken breast
groups had the least total growth compared to the
others.
S The beef liver had a greater total growth compared
to the egg yolk in heads, but egg yolk was greater
than beef liver in bodies. This slight variation shows
that these amounts of vitamin A were not large
enough to show any noticeable differences.
Conclusion
S Results confirm hypothesis
S As we predicted, we found that the group of
Planarians fed turkey liver grew the most and
the Planarians fed chicken breast grew the least.
Acknowledgements

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Planaria 1

  • 1. S Tomomi Shore, Shawn Cyran Biomedicine Program, Academies@Englewood March 5, 2015
  • 2. What is a planarian?• Classified in the Platyhelminthes phylum or “flat worms” • Carnivores • No anus and eats using pharynx in middle of its body • Has central nervous system Flat worm with ability to regenerate
  • 3. What is a planarian? Continued • Sexually reproduce and asexually reproduce through division • Hypotheses for regeneration: • Epimorphosis- neoblasts • Morphallaxis- morphogens give instructions • Polar Coordinate model- molecules act as “zip code”
  • 4. S Retinoids (found in animals) and carotenoids (found in plants) are two subgroups of vitamin A group. S Retinoids provide immune, inflammatory, genetic, and reproductive benefits and are important to cell growth and embryonic development. Retinol
  • 5. Purpose/Hypothesis S Since Vitamin A is essential to growth and maintenance of the body, we studied the effects of foods with varying amounts of vitamin A on Planarian regeneration. S We hypothesized that Planarians fed greater amounts of vitamin A would show greater regeneration compared to others fed less.
  • 6. Vitamin A Retinoid Found in Foods Food Vitamin A per 100g Chicken Breast 50 IU Egg Yolk 1,442 IU Beef Liver 53,400 IU Turkey Liver 72,350 IU S We predicted that turkey liver would have the greatest growth while chicken breast would have the least because turkey liver has the most vitamin A, and chicken breast has the least.
  • 7. Methods Feed planarians designated food one week before amputation Place (using pipette) Planarian on ice mount for 40-80 seconds Put Planarian on coffee filter on petri dish to surgically amputate the head Remove coffee filter, place graph paper under petri dish
  • 9. Results: Head Pieces Growth Food Day 0 Day 1 Day 5 Day 6 Day 12 Turkey Liver 0 0.15833 3333 1.43333 3333 1.65 1.93333 333 Beef Liver 0 0.11666 6667 0.66666 6667 0.76666 6667 1.38666 6667 Egg Yolk 0 0 0.73333 333 0.93333 333 1.33333 33 Chicken Breast 0 0.25333 3333 1 1.01666 6667 1.2
  • 10. Food Day 0 Day 1 Day 5 Day 6 Turkey Liver 0 0.116666 667 1.916666 667 2.3 Beef Liver 0 0.063333 333 1.283333 33 1.283333 33 Egg Yolk 0 0.173333 33 0.84 1.79 Chicken Breast 0 0.1 0.716666 66 1.12 Results: Body Pieces Growth
  • 11. Graphs: Heads Growth 0 0.5 1 1.5 2 2.5 Day 0 Day 1 Day 5 Day 6 Day 12 Turkey Liver Beef Liver Egg Yolk Chicken Breast
  • 12. Graphs: Bodies Growth 0 0.5 1 1.5 2 2.5 Day 0 Day 1 Day 5 Day 6 Turkey Liver Beef Liver Egg Yolk Chicken Breast
  • 13. Analysis S In both the heads and bodies, the turkey liver groups had the greatest total growth compared to the others. S In both the heads and bodies, the chicken breast groups had the least total growth compared to the others. S The beef liver had a greater total growth compared to the egg yolk in heads, but egg yolk was greater than beef liver in bodies. This slight variation shows that these amounts of vitamin A were not large enough to show any noticeable differences.
  • 14. Conclusion S Results confirm hypothesis S As we predicted, we found that the group of Planarians fed turkey liver grew the most and the Planarians fed chicken breast grew the least.