Embryo Transfer


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Embryo Transfer

  1. 1. Embryo Transfer in Beef Cattle Ms. Bailes AGSC 336 – Advanced Animal Science
  2. 3. Objectives <ul><li>Define embryo transfer </li></ul><ul><li>Explain the steps of embryo transfer </li></ul><ul><li>List the advantages of embryo transfer </li></ul><ul><li>List the disadvantages of embryo transfer </li></ul>
  3. 4. What is Embryo Transfer? Collecting embryos from a superovulated donor cow... … and transferring the embryos to synchronized recipients to complete the gestation period.
  4. 5. The Donor Cow <ul><li>Top quality female, free of reproductive abnormalities or genetic defects, proven maternal qualities. </li></ul><ul><li>Personal goals/preferences play large part in donor selection. </li></ul><ul><li>Marketability of the donors calves. </li></ul>
  5. 6. Superovulation of Donor Day 0 to Day 4 <ul><li>Follicle Stimulating Hormones </li></ul><ul><li>(Known as FSH) </li></ul><ul><li>Injections 2x daily, 4 days </li></ul><ul><li>(Day 0 starts 8-14 days following estrus cycle.) </li></ul>
  6. 7. Superovulation--Day 3 <ul><li>Prostaglandin injection causing Corpus Luteum regression to bring on estrus. </li></ul>Result: Multiple Folicles Result: Multiple Folicles
  7. 8. Superovulation--Day 5 <ul><li>Onset of estrus </li></ul>
  8. 9. A.I. of Superovulated Donor <ul><li>Artificially Inseminate when in standing estrus, usually 5 days after superovulation. </li></ul><ul><li>Usually multiple inseminations at 12, 24, 36 hrs. after onset of estrus. </li></ul><ul><li>Preferably, more than one straw of high quality semen. </li></ul>
  9. 10. Embryo Recovery from Donor <ul><li>Approximately 7 days after breeding. </li></ul><ul><li>Easy, takes less than 30 minutes. </li></ul><ul><li>Insert foley catheter with inflatable balloon into donor’s uterus. </li></ul><ul><li>Flushing solution (saline) is introduced into each uterine horn. </li></ul>
  10. 11. Recovery of Embryos <ul><li>Solution filled uterine horn is gently massaged. </li></ul><ul><li>Fluid containing the embryos is drawn back out and collected though a filter into a holding cylinder. </li></ul>
  11. 12. Embryo Processing <ul><li>After 20-30 minutes embryos located with stereoscopic microscope. </li></ul><ul><li>Embryos are washed and transferred to holding medium. (Procedure generally repeated 3 times.) </li></ul><ul><li>Embryos are evaluated for state of development and quality. </li></ul><ul><li>Initially classified: “good” or “bad.” “Good” embryos are further classified. </li></ul>
  12. 13. The Recipient <ul><li>Reproductively sound, good mothering ability. </li></ul><ul><li>Good overall health, nutrition. </li></ul><ul><li>Must be synchronized to receive the embryo. </li></ul>
  13. 14. Synchronization Protocol--Day 0 <ul><li>Insert intravaginal CIDR. </li></ul><ul><li>Administer 2 cc combo Estradiol Benzoate and Progesterone. </li></ul>
  14. 15. Day 7 <ul><li>Remove CIDR </li></ul><ul><li>Administer 5cc Prostaglandin </li></ul>
  15. 16. Day 8 <ul><li>Final Injection: </li></ul><ul><li>1 cc Estradiol Benzoate </li></ul>
  16. 17. Day 8 through Day 10 <ul><li>Day 8 pm through Day 10am: </li></ul><ul><li>Observe for onset of estrus. </li></ul>
  17. 18. Day 16--Transfer Embryo <ul><li>Transfer Embryo into synchronized recipient who has come into estrus . </li></ul><ul><li>Procedure is simple, </li></ul><ul><li>much like A.I. </li></ul>
  18. 19. Activity <ul><li>Each card has a step in the embryo transfer process. </li></ul><ul><li>Put yourselves in the proper order of embryo transfer and then explain the process. </li></ul>
  19. 20. Advantages of E.T. <ul><li>Increased number of calves of genetically </li></ul><ul><li>superior cows. </li></ul><ul><li>Increased marketing opportunities-- </li></ul><ul><li>offspring AND embryos. </li></ul><ul><li>Ease of Import/Export. </li></ul><ul><li>Embyros can be stored indefinitely. </li></ul>
  20. 21. Disadvantages of E.T. <ul><li>Increased expenses and higher break-even costs for </li></ul><ul><li>calves. </li></ul><ul><li>Estrus detection required. </li></ul><ul><li>Synchronization of recipient with donor. </li></ul><ul><li>Specialized equipment and trained personnel. </li></ul><ul><li>More expensive and time consuming than </li></ul><ul><li>traditional reproductive methods. </li></ul>
  21. 22. In Summary… <ul><li>E.T. is not for every herd. </li></ul><ul><li>Each female born has thousands of potential eggs. </li></ul><ul><li>Complete the crossword puzzle which is a summary/review of what we learned today. </li></ul>