Mise-en-scene refers to everything within the frame of a shot that can be used to tell the story or provide context. This includes elements like color, costumes, props, location, blocking/performance, and lighting. The document provides examples of how each of these elements can be symbolic and help establish mood, character, and cultural context. Lighting specifically is discussed as either high-key, which provides even lighting, or low-key, which creates more dramatic shadows and contrast through targeted lighting.