1. Todd Byrne
hiretoddbyrne@gmail.com
401-559-8754
Summary:
I possess over five years of experience in laboratory research areas, such as
enzymology, NMR, organic chemistry, and biochemistry. I have multidisciplinary
experience. Consequently, I can execute procedures in fields of biophysics,
biochemistry, microbiology, and biology.
Work History:
I. SBH Sciences: Research Scientist, Mar 12, 2015-Nov 2, 2015:
Natick,MA
Achieved goals:
- Helped prepare and execute experiments for a number of preclinical studies for human
pharmaceutical companies
II. Eternogen: Research and Operation Scientist, Aug 1, 2013- Feb 20, 2014:
Columbia, MO
Achieved goals:
- Produced sterile collagen for a human clinical trial in a GLP manner,
exponentially increased shelf life of the collagen product
Applied Skills:
- Preformed routinely aseptic techniques: autoclaving reagents, sterile filtering, use of
laminar flow hood (biological hood), maintain clean work environment
- Wrote SOPs for the operation of the GLP laboratory
- Preformed and Developed biochemical assays and production protocols: developed a
plate reader-based fibrilogenesis assay, advised on the production of stable collagen,
dialysis, and precipitation.
III. University of Missouri: Science Tutor, Oct. 2012- July 2013:
Columbia, MO
Applied Skills:
- Taught students using my extensive knowledge of biology, chemistry, biochemistry,
and molecular biology.
IV. University of Missouri: Biochemistry Research Assistant, Aug. 2008- Dec.2012:
Columbia, MO
Achieved goals:
- Published the first chemical structure of Matrix Metalloproteinase-12 (MMP-12) bound
to a
triple helical peptide (THP) collagen mimic and published on other MMP-12
properties ,such as dynamics, inhibitor binding, and micelle binding
- Synthesized and Crystallized a novel amadori sugar (Sorbosamine)
Applied Skills:

2. - Preformed routinely microbiological techniques: aseptic techniques, media
preparation, autoclaving, minimum media growth, protein expression, positive and
negative selection techniques
- Devised, analyzed and presented the resulting data of in-vitro pharmaceutical
enzymological applications (standard and novel): Michaelis-Menten, single-progress
curves, inhibition analysis, and thermodynamic stability assays
- Produced superior protein and small molecule purification yields through these
techniques:
Chromatography (HPLC, Ion exchange, size exclusion, and silica columns), SDS-
PAGE, -Dictated and direct over general lab safety, cleanliness and techniques:
pH meter, UV-Vis spectrophotometer, ordering and care of chemicals, proper
protective equipment, Fire-Health-
Reactivity
- Executed leadership qualities constantly in the workplace and in the community (see
below)
Specialized Instrumental Skills:
- Operate precisely the following Instruments: HPLC, NMR, plate readers,
electrophoresis, photospectrometer (UV-Vis),MALDI, and general lab equipment
- Can operate Windows OS(s), Linux OS(s), and Mac OS(s), Microsoft Word,
PowerPoint, Excel expertly
V. University of Vermont: Laboratory Scientist, Aug 2007- May 2008:
Burlington, VT
Applied Skills:
- Completed biochemical assays, such as ELISA, SDS-PAGE, Enzyme Kinetics, and
hematology
Other Work History:
A. Inspiring Minds (Providence, Rhode Island)- Mathematical/Logic/Science
Educator, Oct. 2014-Present
Achieved goals:
-Educate youth on the principles of Math
Applied Skills:
- Taught Mathematical operations and concepts
B. DNAcampains-2014 Elections
-Persuade people to vote for Joe Courtney, Dan Malloy, Mae Flexer, and Ted
Kennedy Jr.
C. Friends of Gina Raimando- 2014 Primary and 2014 Election
- Persuade people to vote for Gina Raimando
-Collect data on voting and analyze and report to supervisors
D. Buzzards Bay Strategies Missouri Early Voting Fund: Canvasser, 2014
Achieved goals:
-Collected collectively over 250,000 signatures,
-Collected personally over 1200 signatures for campaign
Applied skills:

3. -Communicated succinctly the political purpose of petition,
-Persuade people to sign petition
Education:
- Attained Master of Science Degree in Biochemistry, University of Missouri;
Columbia, MO 65211
Graduation: December 2012
- Attained Bachelor of Arts in Biology Degree, Castleton State College, Castleton VT
05735
Graduation: May 2006
Teaching/Management:
- Attended the University of Missouri: Graduate Student Teaching Assistant
- Worked for Castleton State College as Tutor of Chemistry
- Worked for the State of Vermont as Chemistry Instructor
Awards:
- Awarded Place among Golden Key Honor Society (International Honor Society)
- Inducted into the Sigma Zeta Honor Society (National Science and Math Honor
Society)
- Presented my scientific work at well-known conferences (see below)
- Received prestigious awards: Awarded a place on the 2009 and
2010 NIH training grant, 2010 receipt of the Dr. Gehrke Jr. Memorial
Scholarship, 2005 receipt of William Feaster Memorial Scholarship
Presentations (Published Abstract):
- Byrne, T. (2011, October). MMP-12 interactions with extracellular matrix
components . 5th (2011) GRASP: NMR symposium on the use of NMR and NMR
techniques for analysis of macromolecular and biochemical
- Byrne, T. (2011, June). MMP-12 interactions with extracellular matrix
components. 35th Steenbock Symposium 'Advances in Biomolecular NMR',
University of Wisconsin-Madison.
- Byrne, T. (2011, March). Characterizing MMP-12 interactions with a substrate
and an inhibitor that involve exosites meeting. 1st Missouri biophysics
symposium: single-molecule biophysics, University of Missouri-Columbia.
- Byrne, T. (2010, October). Characterizing MMP-12 interactions with a substrate
and an inhibitor that involve exosites. 4th (2010) GRASP: NMR symposium on the
use of NMR and NMR techniques for analysis of macromolecular and biochemical
systems, Lawrence, KS.
- Byrne, T. (2010, October). Characterizing MMP-12 interactions with a substrate
and an inhibitor that involve exosites. 45th (2010) American Chemical Society's
Midwest regional meeting, Wichita, KS.

4. Selected Publications:
-Byrne, Todd. "Patent WO2014138021A1 - Injectable in Situ Polymerizable Collagen
Composition." Google Books. March 4, 2014. Accessed February 19, 2015.
http://www.google.com/patents/WO2014138021A1?cl=en.
- Byrne, Todd. Structural Interactions Sites of Matrix Metalloproteinase- 12 with
Collagen Triple Helix, Micelles, and a Natural Product from Green Tea. MS
thesis. University of Missouri-Columbia, 2012. Columbia, MO: University of
Missouri- Columbia, 2012. Web.
- Liang, X., Arunima, A., Zhao, Y., Bhaskaran, R., Shende, A., Byrne, T. S., Fleeks,
J., Palmier, M. O., and Van Doren, S. R. (2010) Apparent Tradeoff of Higher
Activity in MMP-12 for Enhanced Stability and Flexibility in MMP- 3, Biophysical
Journal 99,
273-283.
References Available Upon Request