2. ASSIGNMENT OF MICROBIOLOGY
Assignment Topic : Colony Characteristics
Submitted To : Sir Fazal Ilahi Butt
Submitted By : Tayyab
Roll Number : 869 / 023808
Program : B.S. 4 Years Degree Program
Discipline : Zoology
Session : 2017-2021 / 7th Semester
Government College Of Science Wahdat Road , Lahore
3. COLONY CHARACTERISTICS
Colony:
A colony is defined as a visible
mass of microorganisms all originating
from a single mother cell, therefore a
colony constitutes a clone of bacteria all
genetically alike.
4. There are many different Characteristics of colony.Som of them
are given as:
5. Size:
The Colonies range in size from
extremely small (pinpoint), measuring only
a fraction of millimeter in diameter, to
large colonies measuring 5 to 10 millimeter
in diameter.
6. Margin:
The colony of bacteria may take
one of several different patterns. It may be
circular, or irregular such as rounded
projections,notches,and threadlike or
rootlike projections.
7.
8. Surface Texture:
The colony surface may
be smooth (shiny,
gelatinous);rough(dull,granular or matte) or
mucoid(slimy or gummy) or wrinkled
Streptococcus pneumoniae Smooth
Mycobacterium tuberculosis Rough
Klebsiella pneumoniae Mucoid
9. Elevation:
Depending on the species, colony
may be thin to thick and the surface may
be flat or it may exhibit varying degrees of
convexity.
10. Optical Features:
Colonies may be opaque,
translucent, or opalescent.
Staphylococci are usually opaque, while many
Streptococcus species are translucent.
11.
12. Chromogensis:
Some bacterial species
produce, and retain water-insoluble
pigments intracellularly, thus causing the
colonies to become colored (pigmented).
Some species which form pigmented
colonies are given on next slide.
13. Flectobacillus major Pink
Serrotia marcescens Red
Chrornobacterium violaceum Violet
Staphylococcus aureus Gold
Micrococcus luteus
Yellow
Derxia gummosa Brown
Bocteroides meloninogenicus
14.
15. Some colonies produce pigments that are
water-soluble; these diffuse into the
surrounding agar and stain it. For instance,
Pseudomonas aeruginosa forms a blue
water-soluble pigment called pyocyanin.
Some pigments are only sparingly water-
soluble and may precipitate in the medium.
For example, Pseudomonas chiororaphis
forms a pigment called chlororaphin which
accumulates in the form of green crystals