Presentation on senna plant and its characetristics, chemical constituencies and more

1. SENOSIDES
Presented by Tulsi ram jabegu
M.Pham 1st (sem.Pharmacognosy and
phytochemistry )
Submitted To .Dr M.Mujeeb M. Pham. PhD
Department of Pharmacy JAMIA HAMDARD
New Deilhi

2. Contents
 Introduction
 Characteristics of sennosides
 Chemical structure of sennosides
 Sennosides contains various parts of senna plant
 Biosynthesis of sennosides
 Extraction and isolation method of sennosides
 Identification of sennosides
 Purification methods
 References

3. Sennoside
 Sennoside is known as senna glycoside or senna
 , is a medication used to treat constipation and empty the large intestine before surgery.
 The medication is taken by mouth or via the rectum.
 It typically begins working in minutes when given by rectum and within twelve hours
when given by mouth. It is a weaker laxative than bisacodyl or castor oil.

4. Characteristic of sennosides
Mol .formula C42H38O20
mol. Wt 862.746g/mol
M.p. 329f
it is brown powder
slight odour and taste solid
(9R)-9-[(9R)-2-carboxy-4-hydroxy-10-oxo-5-
[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-
(hydroxymethyl)oxan-2-yl]oxy-9H-anthracen-9-yl]-4-
hydroxy-10-oxo-5-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-
(hydroxymethyl)oxan-2-yl]oxy-9H-anthracene-2-carboxylic
acid
2D Structure of sennosides

5. Chemical structure of sennosides
 Sennosides are obtained from the dried leaflets of Cassia senna (Cassia acutifoliaj Delile
and Cassia angustifolia Vahl (Fam: Leguminosae).

6. Chemical structures cont…

7. Sennoside B content in various parts of
C. angustifolia.
Part Sennoside B content %
Filament and Anthers 7.2
Carpels and ovaries 5.8
Petals 5.2
Sepals 4.7
Flowers 4.3
Flower stalk 3.2
Leaflets 2.8
Pericarp 2.4
Stems 0.2
Roots 0.05

9. Cont…

13. Purification of Sennosides

14. Purification cont.…
 HPLC:
a. Meta. hod :isocratic method
b. Mobile phase :ethanol:water:acetic
acid:tetrahydrofuron. (60:38:2:2).
c. Detection:25μ nm.
Further purification can be done by:
Column chromatography
GC-MS

15. References
 www.sciencedirect .com.
 www.slide share.com
 www.pubmed.com
 Google image