Thank you for teacher and for university to publish my presentation about medicinal herbs and thank your for god and my parent

1. Kurdistan Region Government
Ministry of Higher Education &Scientific Research
Soran University
Faculty of Education
Basic Education School
Changes in phenolic compounds during the growing season in some wild herbs
 Subhi Mehmood Ismail
Prepared By : Supervisor :
 Mohamad Saadatian
2021-2022

2.  Introduction
 Material And Method
 Result and Discussion
 Conclusion
 Recommendation
Reference
Content

3. Introduction
This study aimed to
investigate the total number
of polyphenols in four
medicinal plants (primula,
ajuga, Stachys,
Verbascum).
Medicinal plants are
considered one of the most
important sources of
medicine globally as a
natural alternative to
synthetic chemicals.
Polyphenols are the
secondary metabolite
in plants that have
beneficial effects and
have a variety of
structures.
Medicinal plants
contain a large
number, and different
types of medicines.

4. Introduction
 It has been estimated that about
35,000 to 70,000 plant species are
utilized for medicinal purposes
globally, of which 6,500 species
belong to Asia.
 Kurdistan is an area famous for
its biodiversity of plants,
particularly in Southern
Kurdistan.
 Medicinal plants and
plant-derived medicine are
widely used in traditional
cultures worldwide.
 medical plants have sundry
biological impacts, such as
anti- inflammatory and
antioxidant properties.
 study aims to determine
what the polyphenols are
like before harvesting,
during harvesting, and
after harvesting and
determine the rate of
medical plants.

5. Material
Name plant sample
1. Ajuga oblongata L.
2. Stschys Vulgaris
L
3. Primula auriculata L
4. Verbascum spp

6. Material
Name of devices
 Spectrophotometer  HPLC (high-performance
liquid chromatography)

7. Method
 We'll collect the plants.
Steps to conduct the test
 We dry the plants and
make them powder .
 We combine the powders
with the methanol detainee
to take out the polyphenols.
 We standardize HPLS devices
for nine types of Polyphenols.
 After standardizing the device, we
will give the sample to the HLPLC
device for the insertion of nine
types of polyphenols .
 Then we use the
Spectrophotometer Device .

8. Standard HPLC

9. Result and Discussion
Plant Time harvesting Galic acid
Caffeic
acid
Chlorogenic
acid
Rutin
Coumaric
acid
Rosmaric
acid
Quercitin
Cinamic
acid
Apigenin
Primula Pre- Flowering 41.91a 12.77fg 236.99e 70.27b 1043.26b 32.94cd 23.61f 6.28d 1.37g
Primula Flowering 8.15d 170.27c 534.73b 54.22c 1477.9.92a 10.49f 32.89e 7.13cd 62cd
Primula Post- Flowering 4.33fg 1.56f 2.8i 3.3g 115.7gh 3.6g 2.86h 0.19f 2.5fg
Ajuga Pre-Flowering 6.77de 37ef 149.56fg 6.86g 274.09ef 35.39c 88.12a 22.44b 12.29efg
Ajuga Flowering 5.95def 54.73e 184.36f 4.34g 55.13h 83.69a 22.45f 8.32c 15.25ef
Ajuga Post-Flowering 24.16b 67e 827.62a 18.58f 437.47d 29.6cd 66.66b 8.56c 62.18cd
Stachys Pre-Flowering 6.39def 450.68a 360.72d 47.37d 379.55de 26.37e 54.99c 3.5e 472.63a
Stachys Flowering 5.79ef 255.85b 232.79e 55.26c 220.84fg 12.86f 35.37e 7.29cd 64.66cd
Stachys Post-Flowering 23.05b 67.19e 420.68c 16.17f 166.93fg 13.13f 39.69d 8.35c 145.13b
Verbascum Pre-Flowering 1.42h 180.31c 130.33g 21.39f 22.47h 5.28g 12.95g 3.65e 133.39b
Verbascum Flowering 17.08c 103.68d 148.63fg 39.12e 910.8c 10.14f 14.98g 31.81a 23.28e
Verbascum Post-Flowering 2.53gh 64.07e 52h 120.38a 450.04d 51.91b 23.45f 22.28b 66.41c
Table (1) shows the results of nine type polyphenols to four medicinal plants in different
time harvesting .
( High value ) ( low value ) ( Decrease to increase) ( Increase to decrease)

10. Result and Discussion
Table (2) Antioxidant capacity

11. Result and Discussion
Table (3) Total phenols

12. Conclusion
This study has shown that plants should be used with the highest polyphenol rates
and have the most medical benefits to achieve effective and beneficial results. If the
rate of polyphenols is high, the activity of antioxidants and polyphenols is higher, so
according to this research.

13. Recomondation
1. It should be tried that the
plants that are not in
Kurdistan and have a good
side should be developed here
to benefit the health side
naturally
2.To try to use any plant that has
benefits in the industry, especially
the plants that are being
researched
3. These Plants should be seen as
the main basis of medical needs
and instead of medical needs
outside the country, they should
be here

14. Reference
1. AHMED, H. M. 2016. Ethnopharmacobotanical study on the medicinal plants used by
herbalists in Sulaymaniyah Province, Kurdistan, Iraq. Journal of ethnobiology and
ethnomedicine, 12, 1-17.
2. AUSTIN, D. F. 2004. Medicinal plants of the world. An illustrated scientific guide to
important medicinal plants and their uses. Economic Botany, 58, 505-505.
3. BRAND-WILLIAMS, W., CUVELIER, M.-E. & BERSET, C. 1995. Use of a free radical method to
evaluate antioxidant activity. LWT-Food science and Technology, 28, 25-30.
4. CHRISTAKI, E., BONOS, E., GIANNENAS, I. & FLOROU-PANERI, P. 2012. Aromatic plants as a
source of bioactive compounds. Agriculture, 2, 228-243.
5. CRAINE, J. M. 2007. Plant strategy theories: replies to Grime and Tilman. Journal of
Ecology, 95, 235-240.