Translocation of proteins into the ER requires a signal sequence on the N-terminal side of the protein to be translocated. This sequence interacts with the translocation complex in the ER membrane to move the protein into the ER. Describe your experimental strategy to identify genes whose products participate in protein translocation into the ER. In class, draw a schematic representation of the experimental strategy. .

1. Translocation of proteins into the ER requires a signal sequence on the N-terminal side of the
protein to be translocated. This sequence interacts with the translocation complex in the ER
membrane to move the protein into the ER. Describe your experimental strategy to identify
genes whose products participate in protein translocation into the ER.
In class, draw a schematic representation of the experimental strategy.