transgenic-sheep-.pptx

1. Biotechnology
Transgenic sheep
Iv – semester
PRESENTED BY
SIVARAMAKRISHNAN.T
II M.SC. MICROBIOLOGY
Reg.no.20201232516121
PRESENTED TO
DR.G.RAMANATHAN GANESHAN
DEPARTMENT OF MICROBIOLOGY
SRI PARAMAKALYANI COLLEGE
ALWARKURICHI

2. Transgenic sheep
1996-DOLLY-the sheep was cloned using cells from a 6yr old sheep's udder by
somatic cell nuclear transfer (SCNT)technology
somatic cell nuclear transfer technology
involves two different cells
ovum somatic cell
(eggoocytes) (skin fibroblast)
-nucleus microinjected with
nucleus transgenic DNA
discarded
cell
enucleated egg discarded
(empty ovum)
lone somatic
nucleus

8. E.g.; inactivating both alleles of a locus or creating
multiple gene changes
 Individual donor cells are fused to an enucleated oocyte with
short-duration electric pulse
 E.g.; two 2.5 kilovolt /cm pulse for 10microseconds
 Used to fuse adult cattle fibroblasts with enucleated oocytes
 The pulse simultaneously indce cell fusion and oocyte
activation
 Blastocyst stage before transferred into the uterus of a
pseudopregant female

10.  Surviving animals produced by nuclear transfer
are healthy
 There is a substantial loss of individual before and
after birth some of the cloned animals display
abnormalities
 Abnormliaties such as increased birth weight
 DNA methylation and histone modification of the
original donor cell is inappropriate maintained in
the cells of the recipient animal

11. USES OF TRANSGENIC SHEEP
it is used as a model for studying
Immunology
Human Blood Clotting Factor Viii
Transplantation
Haematology
Biological Product Manufacturing
Recombinant DNA
Drug Production In Milk

12. Advantages
 Site-specific Genetic
Changes Are Possible
 All Offspring Are Transgenic In DNA
Microinjection

13.  Transgene Integration Occurs At Random Sites
 Unstable Things Are Formed
 Expression Is Always Constrained
 No.Of Generations Required
 Much Devoted Id Needed
 Nucleic From Somatic Cell Is Used
 Develop Perfect Cloning Of Livestock

14. DISADVANTAGES
Difficult Procedure
Failed In Vitro Fertilization
Expensive

15. Industrial biotechnology and commodity
products
 transgenic animals are being
used for production of recombinant
proteins in milk, egg, white, blood, urine
seminal plasma ,and silk worm cocoons

 The amount milk produced by animals
(L yr-1) are 8000 per cow, 1000 per
goat, 300 per sheep, and per rabbit