this presentation is about the sample collection, storage and trasnport of specimens for microbiological analysis to a clinical laboratory. this presentation is suitable and usefull for those who are working in aclinical microbiology laboratory like technicians, laboraotory scientists, nurses and phleobotamist.
2. What is Microbial culture?
Laboratory microbial culture means “Selectively Growing” infectious M.Os
If infection is suspected, before going to start random Antibiotic therapy
Doctors should advice the patient to perform culture and sensitivity testing.
3. Importance of Microbial culture
Determine the CAUSE of infectious diseases
To find the right antibiotic for infection control
To reduce antimicrobial resistivity
4. Anti-microbial Resistivity (AMR)
Major public health threat Worldwide
It is not a future prediction
But happening now in every region and affect anyone
WHO: 10 MILLION deaths/year are expected by 2050 due to AMR
5. Why Physicians prefer culture?
Wrong Antibiotics use will pull you down 6 feet
Underground
10. List of Microbial culture performed in HML
All Body fluids culture and sensitivity
Wound/pus and all kind of swab culture and sensitivity
Tissue and biopsy culture and sensitivity
Stool culture and sensitivity
Fungal culture and sensitivity
Water and food culture for infectious microorganisms
Hospitals OT, Equipment, beds and surfaces sterility checking for microbes
All kind of bacterial and fungal staining tests.
11. Importance of Specimen collection, labelling,
transport and storage
Improper specimen collection, labelling, transport and
storage leads to false laboratory investigation, long hospital
stay, wrong treatment, waste of time and economic loss.
Right Specimen Right Lab Report Right Treatment
12. Guidelines for Specimen Collection
Aseptic precautions
Anatomic sites and locations
Adequate amount/volume
Proper timing
Clinical laboratory request form
Use of proper container
Instruction to the patient
Before the administration of antibiotics
Avoid contamination of specimens
Use of transport media
Proper handling ,labelling and transportation
13. SPECIMEN TRANSPORT
Specimen transport condition
Within 2 hours of collection
Containers should be leak-proof
Separate section for paperwork
Special preservatives or transport
media
Biohazard label
14. Criteria for rejection of specimens:
Say “No” to process every sample which do not fulfill the criteria for proper sample collection, transport and storage.
Sample must be rejected if:
Missing or inadequate information and request form
Insufficient quantity
Specimen collected in an inappropriate container
Contamination suspected
Inappropriate transport or storage
Unknown time delay
15. Workflow in HML Microbiology Dept
Example: CSF
Day 1st
Physical Examination:
Biochemical Examination
Microscopic Examination
Staining: Gram stain, AFB, India Ink and wet preparations
(A primary report is given to the physician to decide the treatment direction.)
Culture of CSF on suitable Culture Media
16. Day 2nd and Onwards:
Examine and report Culture result
Post staining
Antimicrobial susceptibility tests
Final report to physician
17. Why other labs fail in proper microbiology report?
Lack of knowledge about microbes and their characteristics
Improper collection, transport and storage
Unsuitable growth environment
Use of Undefined and non-selective culture media
Contamination
No Growth reporting
18. Our Future Aims and Goals
To aware the society about the importance of C&S
To educate people about the harm in misuse of antibiotics
To provide clinical microbiology services in all provinces of AFG
To find the AMR level in the people of AFG
To reduce the AMR level in AFG