Learn how the new Thermo Scientific LC-MS triple quadrupole mass spectrometers incorporate unique design features that improve analysis of food matrices for veterinary drug and pesticide residues.

1. 3 Ways our New LC-MS
Triple Quads Improve
Residue Analysis

2. Complex
Matrices
Large
Sample
Numbers
Low
Concentration
Today’s
challenges in quantitative mass spectrometry
demanded that we elevate
Small
Sample
Amounts
triple quad capabilities

3. Thermo Scientific™
TSQ Endura MS
Extreme quantitative
value with best-in-class
sensitivity
New Generation
Triple Quadrupole LC-MS systems
Thermo Scientific™
TSQ Quantiva MS
Extreme quantitative
performance with unrivaled
attogramlevel sensitivity

4. How do our new
Triple Quadrupole systems
improve residue analysis?

5. SPEED

6. Challenge
High volume multi-residue
analyses in short run times
Solution
500 SRMs per second

7. 14000000
12000000
10000000
8000000
6000000
4000000
2000000
0
High sensitivity at every speed
9.9 SRM/sec 47 SRM/sec 166 SRM/sec 333 SRMs/sec 500 SRMS/Sec
Peak Area
Traditional
Triple Quads
decline in
sensitivity, even
at lower SRMs
per second
New Thermo Scientific™ Triple Quadrupole systems
maintain sensitivity up to 500 SRMs per second

8. More points
across the peak
means more
reproducible
results and
allows for faster
run times
6000000
5000000
4000000
3000000
2000000
1000000
0
250 Transitions
Monitored Simultaneously
167 SRMS/second 500 SRMS/second
10 points across the peak
1.75 1.8 1.85 1.9 1.95 2 2.05 2.1
Signal
Time (minutes)
3 points across the peak

9. Analysis of 250 pesticides
> 500 SRMs
5 ppb in onion matrix

10. Scans Across Peaks
500 SRMs simultaneously (not scheduled/timed)
Sufficient number of data
points for reproducible assays

11. 7.00E+07
6.00E+07
5.00E+07
4.00E+07
3.00E+07
2.00E+07
1.00E+07
0.00E+00
0.8 1 2 5 10 20 50
Comparison of Area
Response vs. Scan Speeds
Ave. Area
Dwell time (ms)
Atrazine
Azoxystrobin
Carbendazim
Simazine
10 ppb in Onion Matrix

12. SENSITIVITY

13. Challenge
Demanding assays requiring
lowest limits of detection
Solution
Unrivaled
triple quad sensitivity

14. Pesticides in extracted spinach matrix
Benfuracarb (10 ppq LOD and 4.5 orders dynamic range)
Chromatograms - CV%:11.8%
Unrivaled quantitation
on TSQ Quantiva MS

15. Compound LOD (ppt) % RSD LOQ (ppt) % RSD
Albendazole 2.47 12.64 12.34 4.11
Oxfendazole 2.44 10.86 12.20 6.89
Sulfadiazine 23.81 17.5 119.05 9.05
Sulfamethazine 12.62 10.62 25.24 10.21
Sulfaquinoxaline 5.98 7.29 11.97 2.47
Thiabendazole 24.19 3.48 48.37 1.65
Tilmicosin 121.79 1.05 243.57 2.16
Trimethoprim 11.91 20.91 23.81 10.12
Tylosin 11.87 12.79 23.74 10.75
LOQ (ppt) – Veterinary Drug Residues
in Milk (n=7) on TSQ Quantiva MS
Exceeds EU
Regulation
No 37/2010
100–1000x

16. Low and Sub-ppt Levels
• Experiment: Analysis of reclaimed
waste water for emerging
contaminants (PPCPs)*
• TIC of 1.75 pg/mL injected standard 1
mL injection with Thermo Scientific™
EQuan MAX Plus™ online sample
prep LC-MS with TSQ Quantiva MS
• Calibration range:
0.06 pg/mL – 1000 pg/mL with a 1mL
injection, that’s 60 fg – 1000 pg on
column.
100
90
80
70
60
50
40
30
20
10
0
Relative Abunduance
9.68
9.79
10.26
6.87
10.37
11.26
9.46
8.17
6.63 7.85
6.01 11.51 12.42 4.34 5.84
4 5 6 7 8 9 10 11 12 13
Time (min)
*Lee Ferguson and Gordon Getzinger, Environmental Chemistry, Duke University, USA

17. 60fg On Column
Calibration range, 0.06 – 1000 pg/mL -
Flutolanil
4.5 orders of magnitude
Ion Ratio overlay

18. Calibration range:
0.01-100 ppt
Metoprolol and Oxadiazon
Metoprolol LOD 100 ppq
Atraton LOD 50 ppq
Tilt LOD 100 ppq
Calibration range:
0.1-100 ppt
Calibration range:
0.05-100 ppt

19. Compound LOD (pg/mL) Compound LOD (pg/mL)
Acephate 0.24 Fluridone 0.12
Allethrin 7.8 Flutolanil 0.06
Ametryn 0.12 Formasulfron 0.12
Atraton 0.12 Halosulfron-methyl 0.12
Atrazine 0.12 Imidacloprid 0.06
Atrazine-desethyl 0.12 Iprodione_a 15.63
Atrazine-desisopropyl 0.24 Iprodione_b 31.25
Azoxystrobin 0.12 Metalaxyl 0.06
Benzotriazole 7.8 Metoprolol 0.24
Bioresmethrin 62.5 Oxadiazon 3.9
Bloc (Fenarimol) 0.24 Pramoxine 0.12
Carbaryl 0.12 Prometron 0.12
Carbendazim 0.12 Propanmide 0.12
DEET 0.98 Quinclorac 7.8
Etofenprox 3.9 Thiencarbazone-methyl 3.9
Fenamiphos 0.12 Thiophanate-methyl 0.24
Fluoxastrobin 0.5 Tramadol 0.06
Quantitation results using Equan MAX
Plus System with TSQ Quantiva MS

20. ROBUSTNESS

21. Challenge
Robust performance for
ongoing reliability
Solution
Buildup prevention by
elimination of neutral clusters

22. Area
Injections
Robustness across >350 injections with
pesticide spiked sample on TSQ Endura MS

23. Initial calibration curve
overlaid with calibration
curve acquired after
300 injections of
pesticide spiked
sample on TSQ Endura
MS (Imidacloprid and
2,4-D)

24. Robustness across 1600 injections
Ave. %RSD 3.5
• Tuesday: 10 ppb standards in
solvent
• Wednesday and Friday: 10 ppb
standards in jalapeno matrix
• LC Run time: 8 minutes
Injection number
Ave. %RSD 3.3
3.00E+07
2.50E+07
2.00E+07
1.50E+07
1.00E+07
5.00E+06
0.00E+00
0 200 400 600 800 1000 1200 1400 1600 1800
tues
weds
fri
5.00E+07
4.50E+07
4.00E+07
3.50E+07
3.00E+07
2.50E+07
2.00E+07
1.50E+07
1.00E+07
5.00E+06
0.00E+00
0 200 400 600 800 1000 1200 1400 1600 1800
tues
weds
fri
Area Area
Injection number

25. How are our systems
delivering these
capabilities?

26. AIM
TECHNOLOGY
The design behind
the capabilities
Active Ion Management
Precision design of all electric fields, optimized
in concert, to produce maximum signal and to
prevent contamination.

27. Novel Neutral Blocker
Captures contaminating
species outside of the ion path,
keeping ion optics cleaner
Tool-less Removal
No tools needed to remove ion
optics, facilitating quicker cleaning

28. Re-designed TSQ Triple Quadrupole Platform
TSQ Endura MS
Extreme quantitative value with best-in-class
sensitivity
TSQ Quantiva MS
Extreme quantitative performance with
unrivaled attogramlevel sensitivity
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