Urine analysis & Hematology Slides

1. 1
Urine Color

2. 2
Gram Positive Bacteria ( purple )
Gram Negative Bacteria ( Pink ) ( Red )

3. 3
Sensitivity Test
Red blood cells in urine under microscope ( may be normal , Microcytic ,
crenated )

4. 4
white blood cells in urine under microscope

5. 5
Epithelial cell in urine

6. 6
Bacteria in Urine
Parasites in Urine
(trichomonas vaginalis ) (trichomonas trophozoite )
(schistosoma haematobium in urine)

7. 7
Spermatozoa in urine
Mucus in urine
Hyaline casts in urine

8. 8
RBC casts in urine
WBC casts in urine
Epithelial cell casts in urine

9. 9
Fatty casts in urine
Granular casts in urine

10. 10
◘ Normal Crystals seen in Acidic Urine :
- Uric Acid in urine
- Sodium Urates in urine

11. 11
- Amorphous Urates
- calcium oxalate dihydrate crystals in urine
◘ Normal crystals seen in alkaline urine :
- Amorphous Phosphates

12. 12
- Triple Phosphate ( ammonium magnesium phosphate )
- Calcium Phosphate
◘ Abnormal Urine Crystals :
- cystine crystals in urine

13. 13
- cholesterol crystals in urine
- bilirubin crystals in urine

14. 14
◘ Hematology
• hemocytometer
• RBC count by hemocytometer
RBC count = N * 5 * 10000 = ( ) rbc in million/ mm3
(RBCs Count (million / mm3
) = no. of RBCs in 5 squares x 10,000)

15. 15
• WBC count by hemocytometer
WBCs Count (thousands/ mm3) = no. of WBCs in 4 squares x 50
♦ Differential WBC

16. 16
- Neutrophil
- neutrophils band

17. 17
- Basophil
- Eosinophil

18. 18
- Monocyte
- Lymphocyte

19. 19
• Blood Film
• Hematocrite

20. 20
Hb Conc. = A540 nm * 36.8
HCT = PCV = Hematocrit

21. 21
• platelets count
- Platelets Count (thousands/ mm3) = Cells counted x 1000
- Total Platelets Count / mm3 = no. of platelets in 5
squares*1000
Platelets under microscope

22. 22
• Blood group

23. 23
• how to read card

24. 24
◘ Cellulose acetate strip & Citrate Agar Gel
Comment :
• Cellulose acetate hemoglobin electrophoresis used to differentiate
between normal and abnormal hemoglobin but it can't differentiate
between different variants of abnormal hemoglobin so you must apply
citrate agar Hb electrophoresis

25. 25
• Citrate agar used to differentiate between different variants of abnormal
hemoglobin it differentiate Hb S and D And differentiate Hb C and E
◘ gel electrophoresis
◘ Slide widal test

26. 26
◘ ESR
◘ CRP