It is a presentation showing the process of doing a prokaryotic genome annotation using RAST server. It is a basic work in bioinformatics field. Genome annotation is total genome analysis of an organism. We can easily do it using bioinformatics tool like RAST server.

1. GENOME ANNOTATION Of Bacillus cereus

2. INTRODUCTION:
Bacillus cereus is a Gram-positive, rod-shaped, aerobic, facultative
anaerobic, motile, beta hemolytic bacterium commonly found in soil
and food. Some strains are harmful to humans and cause foodborne
illness, while other strains can be beneficial as probiotics for animals.
In this project, we are going to annotate Bacillus cereus NC7401
strain with the help of RAST web server.
RAST (Rapid Annotation using Subsystem Technology) is a fully-
automated service for annotating complete or nearly complete
bacterial and archaeal genomes. It provides high quality genome
annotations for these genomes across the whole phylogenetic tree.

3. PROCEDURE:
1. First of all, we have to enter to
this link which is RAST homepage
http://rast.nmpdr.org/

4. PROCEDURE:
2. Then, we have to log in to our
account or, to create an account
and then log in to it.
3. There is an “upload a new job”
on the page and click it.

5. PROCEDURE:
4. On that page there is a
“Choose File” option. By clicking
that option we upload a
prokaryotic genome because
RAST server only annotate the
prokaryotic genome. As I have
worked with Bacillus cereus, we
upload its genome.
5. To upload genome, we have to
first download it from NCBI
website. We download it in
FASTA format.

6. PROCEDURE:
6. After uploading the genome
on RAST server, we have to
provide the page with taxonomy
ID of the organism. Then click
the option for filling in form
based on NCBI taxonomy ID.
Then click the option “use this
data and go to step 3”.

7. PROCEDURE:
7. In step 3, we have to fill up the
option of some features we want
to consider like “Fix the
frameshift” and “Fix the errors”.
After finishing all these, click the
option “upload a genome” and
wait for the result.

8. PROCEDURE:
8. When the job is done, an email
will be sent to the job uploader
and it would be shown on the
Jobs overview option. We can see
the job’s details on the server
and download it.

9. PROCEDURE:

10. PROCEDURE:
As a result, you will get a doc file
containing annotation result of
genome of Bacillus cereus.

11. REFERENCES:
1. http://rast.nmpdr.org/rast.cgi?page=JobDetails&job=613918
2. https://www.ncbi.nlm.nih.gov/pubmed/
3. https://www.ncbi.nlm.nih.gov/nuccore/AP007209.1