The document discusses research being conducted on the Hyla andersonii frog species. Samples were taken from populations in three states and genetic analysis was performed to measure allelic variation and test hypotheses about population fragmentation or migration. Various genetic markers and primers were tested through polymerase chain reaction and gel electrophoresis, with some primers producing clearer bands than others. The research is being advised by Drs. Camper and Zwiers and funded by the REAL Program at FMU, with collaboration from USC-Columbia to further analyze gene sequences.

1. Dr. Jeff Camper, PhD
Dr. Paul Zwiers, PhD
Shahbaz Mushtaq

2.  Hyla
andersonii
 1.5-2 inches in
length
 Green with dark
stripes
 Large toe pads
 Orange/Gold
Markings on legs

3.  Bushy
areas
 Shallow ponds
 Thick moss
 Terrestrial/near water
 Low pH, 3.8-5.9

4. • New Jersey
• North/South Carolina
• Florida/Alabama
Figure from Anderson
et. al. 2004

5.  Fragmentation
population.
of a larger historical

6.  Migration
and colonization of new habitats.
Figure from Anderson
et. al. 2004

7.  Samples
from each location
 Genetic information to measure allelic
variation
 Genetic signal supports which hypothesis

8. Data from Anderson
et. al. 2004

9.  DNA Analysis
 Polymerase
Chain Reaction (PCR)
 Gel Electrophoresis

10.  Mitochondrial
Genes used in past
 Results from mitochondrial genes
inconclusive
 Additional Information needed

11.  Test
new primers
 Amplify new gene regions
 Run gel electrophoresis
Temperature
tRNApheL/tRNAvalH
16Sh/12L1
12Sm/16Sa
16Sc/16Sd
MVZ15-L/CytbAR-H
MVZ25-L/CytbAR-H
42
44
46
48
50
52
55

12.  Presence
of bands
 Length of bands
Temperature
42
44
46
48
50
52
55
tRNApheL/tRNAvalH
x
x
x
x
x
x
x
16Sh/12L1
x
x
x
x
x
x
x
12Sm/16Sa
x
x
x
x
x
x
x
16Sc/16Sd
x
x
x
x
x
x
x
MVZ15-L/CytbAR-H
o
o
x
√
o
o
o
MVZ25-L/CytbAR-H
x
x
x
x
x
x
x

13.  Changing
primers
 New PCR machine
 Collaboration with USC-Columbia to
sequence gene regions

14.  Advisors
Dr. Jeff Camper, PhD
Dr. Paul Zwiers, PhD
 Funding
REAL Program at FMU
 Collaborators
Dr. Joseph Quattro, PhD, USC-Columbia
Mark Roberts, USC- Columbia
Dr. Pearl Fernandes, PhD, USC-Sumter