2-4-2021

1. KUVEMPU UNIVERSITY
PG Department of studies and Research in Biotechnology
Sahyadri Science College, Shivamogga
Seminar on
“Water microbiology .”
Under the Guidance of
Ms. Syeda Misbaa Fathima
Department of Biotechnology,
Sahyadri Science College,
Shivamogga
Presented by:
MS. Khamar taj khanum
I sem, I MSC
Sahyadri science college,
shimogga.

2. Contents :
1. Introduction.
2. Methods.
3. Tests.
4. Analysis.
5. Conclusion.
6. Reference.

3. Introduction :
 Water microbiology is the scientific discipline that
is concerned with the study of all biological
aspects of the microorganisms.
 Microbes such as bacteria, alate, protozoa,
viruses,fungi, Are examined.
 These microorganisms may be Pathogens or non
pathogens
 The enumeration Microbes in water is done by
Numeras techniques

4. 1. Escherichia colis
2. Enterococcus faecalis
3. Clostridium welchii
4. Pseudomonas aerogens
Major water contaminants:
Bacterial examination of water:
Turbidity, taste, odor of water is to Be check before, to
ensure that water needs to go through the other
standard test or not.
Here, industrial water, potable water, sewage
water, ground water are tested.

5. Coliform bacteria [E-
coli like] are the most
often used indicator
bacteria.
Contamination happens when
water comes in contact with,
1. Faecal matter.
2. Sewage water.

6. Problems
1. Numerous water born pathogens.
1. Individual pathogen number may be too low to
detect in a reasonable sized water sample
2. Isolation And detection of some pathogens can
take several days, weeks, or months

7. Water borne diseases, causative agent, mode of
transportation, mode of infection.

8. Purification of portable water:
Portable water purification devices
Technique enclude heat, filtration, Charcoal
adsorption,Uv disinfection, distillation & floculation in
combination.

9. Nano-scientists develop new kind of portable
water purification system

10. MPN (most probable number) Test :
Presumptive
test

11. 1. Confirmed
test
2. Compleated
test

12. Presumptive test:

13. Presumptive test
Results

14. Confirmed test
results

15. Compleated test,
microscopic
observation :

16. Multiple tube method
One of the oldest methods is called the multiple tube
method.
In this method a measured sub-sample (perhaps 10 ml) is
diluted with 100 ml of sterile growth medium
and an aliquot of 10 ml is then decanted into each of ten
tubes.
The remaining 10 ml is then diluted again and the process
repeated.
At the end of 5 dilutions this produces 50 tubes covering the
dilution range of 1:10 through to 1:10000.

17. ATP testing
 The ATP bioleuminisence System proposes a New way of
measuring Cellular material by measuring ATP levels which
are expressed In realative light units (RLU).
 ATP bioluminiscense assay is has been Increasingly used to
assess the microbiological safety of hospital environment.

18. Plate count method
There are two
methods:
a. Spread Plate
b. Pour Plate

19. Pour plate method
And
spread plate
method

20. Membrane filtration technique
1. Sample passing
through sterile
membrane.
2. Brought in contact
with M-endo
medium.
3. Number of colonies
of coliforms are
counted after 24
hours.

23. Pathogen analysis
Coliforms and other Indicator organisms
They are always present when enteric pathogens or
viruses are detected in water testing.
Its presence in a sample of drinking water means that
the water is unsafe for human consumption.

24. Conclusion

25. Bibliography
1.Aseptic Laboratory Techniques: Plating Methods, Erin R.
Sanders1, doi: 10.3791/3064 Published: May 11, 2012
https://www.jove.com/video/3064/aseptic-laboratory-techniques-plating-
methods
2.Perfecting Your Spread Plate Technique
Diane Hartman, J Microbiol Biol Educ. 2011; 12(2): 204–205.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577269/
3.Bacteriological water analysis, Baydaa A. Hassan University Of Kufa
October 2018,
https://www.researchgate.net/publication/330401314_Bacteriological_water_an
alysis
4. Drinking Water Microbiology Marylynn V. Yates, Published online 2019 Aug
28.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577269/