isolation,identification & estimation of RUTIN

1. Presented By :-
Prashant Jatkar
Prashant Ingawale
Gaurav Deshpande
Yogesh Pawar
Aadseh Khandekar

2. Isolation, Identification &
Estimation OF
Phytoconstituents Of Rutin

3. Content
1. Introduction
2. Isolation
3. Identification
4. Study Of Phytoconstituents
5. Study By Graphs

4. REFERENCE
Therotical book of phamacognosy &
phytochemistry by Trease & Evans
Therotical book of pharmacognosy
& phytochemistry by T.E Wallis

5. Introduction
Rutin Is Also Called as Rutoside
Rutin is Naturally Occuring flavonoid
present in many foods
eg. Grapes, Orange
Chemical Formula: C27H30O16

8. Estimation Of Rutin
•The isolated rutin shown by HPLC
technique.
•It is compared with standard rutin using
combination of Methanol : Water {1:1}

9. HPLC Chromatograph Of
Rutin

10. Identification TEST
 Rutin + FeCl3 Dark green colour
 Rutin + Lead acetate Orange Yellow ppt
 Rutin + Antimony Trichloride Orange Yellow ppt

11. Uses
 Bleeding.
 Blood vessel disease.
 Prevention of mouth ulcers.
 cancer treatments.
 Varicose veins.
 Anti-oxident.
 Anti-inflametory

12. Thank You
!!!!