Rice forms a major part of the diet, which caters to around sixty percent of the population across the globe. Later on, transgenic approaches were utilized to generate various designer crops with increased stress tolerance and nutritional quality. However, due to regulatory issues with transgenic approaches and the invention of genome editing technologies, most of the current research has been focused on precisely knocking out target genes. Since rice was domesticated thousands of years ago, the goal has been to increase grain yields as much as possible. Among the many factors that can influence grain yield, there are a number of genes that influence this trait, and environmental factors play an important role as well. Additionally, rice researchers all over the world are working on enhancing the nutrient content of rice in order to counteract nutrient malnutrition in the population as a whole Traditionally, a number of strategies have been used to increase yields, including the use of high-yielding varieties bred through molecular breeding, the use of chemical fertilizers, and the use of pesticides. We present this study as an experimental investigation of CRISPR-Cas9 as an effective tool to knock out core genes of interest through Agrobacterium-mediated transformation of rice calli into mutants, as well as designing vectors for CRISPR-Cas9-mediated gene knockouts.