1. Curriculum Vitae
Miss Pembi Sherpa Lama
 Permanent address:- Listi-3,Chhyangsing,Sindhupalchowk,Bagmati,Nepal
 Contact no.:- +9779843158402, +9779741209326
 E-mail:- pembisherpalama@gmail.com
OBJECTIVE
“Work-out more, conduct effective experiment after full analysis and develop the innovative technology”
PROFESSIONAL PROFILE
 2014- Till now Research Assistantin Seed Scienceand Technology Division
(SSTD),
Nepal Agricultural Research Council (NARC)
Khumaltar,Lalitpur,Nepal
 2013- 2014 Teacher in Shree Ugrachandhi
Maddhyamik Biddhyalaya
Listi-6,Sindhupalchowk,Bagmati,Nepal
 2009-2012 Executive and public representingmember of
Biotech Association of Nepal for Research &
Development (BANRD)
EDUCATION
 2013 (3.60/4.00 CGPA) Bachelor of Science in Biotechnology
SANN International College,
Gairidhara, Kathmandu, Nepal
 2007 (64%) Intermediate of Science
Centre for Innovative Technology (CIT) Higher Secondary School,
Battisputali, Kathmandu, Nepal
 200 (80.25%) School Leaving Certificate
Tri-Star English Secondary School,
Lokanthali-16, Bhaktapur, Nepal

2. RESEARCH PROJECTS
 November 2012-September 2013 Completed project entitled “Study of Epilepsy using different
Approaches:” as a part of undergraduate thesis work.
i. Assessing level of knowledge & understanding about epilepsy among college students,
ii. Screening of C121W mutation in people living with epilepsy in Nepal &
iii. Prioritization of disease candidate gene for Temporal Lobe epilepsy using Max Planck disease
candidate prioritizer under the supervision of Miss Praphul Shakya atSANN International
College and Research Institute.
 Experimental analysis of growth possibility of potato through Apical meristem culture by varying the
millimeter size of meristem of potato atGREAT Nepal, Baneshwor and SANN international College,
Kathmandu in January-March 2012.
 Management of Cardamom plant culture at GREAT Nepal, Baneshwor in April 2012.
 Wine production and ageing at SANN International College,Kathmandu in December 2011-January 2012.
 Chicken embryo fibroblast cell culture at SANN International College,Kathmandu in December 2011.
 Vermiform composting for improvement of soil at SANN International College,Kathmandu in June 2011.
 Detection of Citrus Greening Disease in Citrus Plant leaves by CTAB method at SANN International
College, Kathmandu in April 2011.
 PCR detection of Rifampicin resistant gene (rpoB) from Mycobacterium leprae in leprosy diseased
Nepali patients at SANN International College,Kathmandu and Leprosy Mission Hospital,Lele, Lalitpur in
August 2010.
 Experimental Analysis of plant hormone Giberellin on germination of Wheat Seed embryo at SANN
International College,Gairidhara,Kathmandu in June- 2010.
 Production of virus free potato seeds of potato at SANN International College,Kathmandu in July 2009.
GRANTS
Ministry of Youth, Government of Nepal Grant: For the project, “The Virus Free Potato Seed Production
program” 2009-2010 which was pilotprojectof Biotech Association of Nepal for Research and Development
(BANRD) by Pre Basic Seed Production.
LABORATORY SKILLS
 Plant tissue culture Techniques: Callus culture, Nodal culture, meristem culture, anther culture.
 Fermentation Technology: Production of wine. I.e. Cider and food making techniques: jam- jelly
production, yoghurt production, Ketch-up production, pizza making and food grading techniques.
 Microbiology: Basic culturetechniques,bacteria isolation,identification,characterization and
transformation.
 Serological Techniques: ELISA, agglutination and immune-diffusion.
 Molecular Techniques: DNA, RNA and protein isolation,purification and quantification,PolymeraseChain
Reaction, electrophoresis,SDS-PAGE, spectrophotometer analysis.
 Chromatographic Techniques: Paper, thin layer,column and ion-exchange chromatography.

3. SEMINARS AND PRESENTATIONS
 Seminar presentation on “Study of Epilepsy using different approaches:”Assessing level of knowledge &
understanding about epilepsy among college students, Screening of C121W mutation in people living
with epilepsy in Nepal &Prioritization of disease candidate gene for Temporal Lobe epilepsy using Max
Planck disease candidate prioritizer at SANN International College,Gairidhara,Kathmandu,Nepal.
 Seminar presentation on “Computer Aided Drug Design” at SANN International College,Gairidhara,
Kathmandu, Nepal.
 Seminar presentation on “Industrial production of Streptomycin” at SANN International College,
Gairidhara,Kathmandu,Nepal.
 Seminar presentation on “Global Scenario and prospects of transgenic crops” at SANN International
College, Gairidhara,Kathmandu,Nepal.
HONORS AND AWARDS
 Merit based scholarship (University/Class Topper in First Semester) of Bachelor courses on 21st May
2009 at SANN International College,Gairidhara,Kathmandu.
 Second Prize Winning team in Inter College Bio-Tech Quiz-2012 organized by Lord Buddha Education
Foundation (LBEF) campus on 4th July 2012.
 Merit based scholarship 10+2 Level, CIT College, Battisputali,Kathmandu.
 School Topper in School Leaving Certificate(SLC) Examination in 2005.
EXTRACURRICULAR ACTIVITIES
 Active participation in differentindoors and out-door games in university Level,.
 1st prize winning team in Quizorganized by Akhil Krantikari Biddhyarthhi Union.
 Participation in Intra-Biotech QuizContest 2011 organized by BIOTECH LEARNERS’ Club on 25th May 2011
at SANN International College,Gairidhara,Kathmandu.
 Participation in TableTennis,Badminton, Cricket, etc.
 Involvement in different Education and Industrial tours.
 Active Participation in extra-curricular activities in School Level.

4. REFEREES
 Mukunda Ranjit, Ph.D.
Academician of National Academy of Scienceand Technology (NAST), Kathmandu, Nepal.
Head of Department of Biotechnology, SANN International College,Gairidhara,Kathmandu,Nepal.
Email:- ranjitmukunda@gmail.com
 Anup Sharma, Ph.D.
Lecturer of Biotechnology Department, SANN International College,Gairidhara,Kathmandu,Nepal.
Email:- a1nup@yahoo.com
 Ms. Praphul Shakya,
Thesis Supervisor
Lecturer of Biotechnology Department, SANN International College,Gairidhara, Kathmandu,Nepal.
Email:- praphulshakya@gmail.com
 Mr. Danda Pani chapagai,
The Then President of Biotech Association of Nepal for Research and Development (BANRD), Kathmandu,
Nepal.
Email:- dpchapagain@yahoo.co.uk