Artificial media animal cell culture classifications such as serum containing media,serum free media,protein free media, chemical Free media.

1. PRESENTATION
ON
ARTIFICIAL MEDIA
SUBJECT:- ANIMAL BIOTECHNOLOGY

2. CONTENT:
■ INTRODUCTION TO ANIMAL CELL CULTURE
■ STEPS TO COLLECT ANIMAL CELLS
■ ANIMAL CELL CULTURE
■ ARTIFICIAL MEDIA
■ CLASSIFICATIONS
■ REFERENCE LINK

3. Introduction to animal cell culture:
■ Animal cell culture is a technique in which the cells are removed
and are allowed to grow in a favorable artificial environment.
■ The removal of tissue can either take place from tissue directly or
from disaggregation by enzymatic or mechanical means before
culture, or they may be originated from a cell line or cell strain
that has been established earlier.
■ Usually, mammalian cells are found to be more delicate and more
susceptible to mechanical damage.
■ They have lesser growth rates and need more complex culture
media along with special substrates.

4. Steps to culture animal cells:
■ Harvest cells
■ Isolation of the cells with the use of appropriate enzymes.
■ In a culture dish with appropriate growth media, the cells are
placed.
■ The culture dish is now kept in incubator for the culture of cells.
■ Cells can be sub-cultured in order to fix the problem or to get the
pure culture.
■ Now, cells are ready to be manipulated or modified for lab
procedures.

5. Animal Culture media:
■ In animal tissue culture, 2 types of culture media are used:
– Natural media
– Artificial media
■ The type of medium relies basically on the type of cells to
be cultured and its objectives.

6. Artificial media:
■ For the following purposes, various artificial media have
been employed:
■ Immediate survival (a balanced salt solution with specified
pH and adequate osmotic pressure)
■ Prolonged survival (a balanced salt solution in addition
with serum, or appropriate formulation of organic
compounds.
■ Indefinite growth
■ Specialized functions

7. CLASSIFICATIONS:
■ Artificial media may be classified into following types:
– Serum containing media
– Serum-free media
– Protein free media
– Chemically defined media

8. Serum containing media:
■ In animal cell culture media, fetal bovine serum is the most
common supplement.
■ In order to supply an optimal culture medium, it is employed as an
economical supplement.
■ Serum supplies carriers water-insoluble nutrients, protease
inhibitors, hormones and growth factors and binds and neutralizes
the toxic moieties.

9. Serum free media:
■ In case of immunological studies, presence of serum in media can
result to serious misinterpretations.
■ In general, these media are specifically designed to promote the
culture of a single type of cell and incorporate specified amounts
of purified growth factors, lipoproteins and other proteins
normally supplied by the serum.
■ As the components of these media are known, thus it is referred to
as ‘defined culture media’

10. Chemically defined media:
■ These media contain ultra-pure inorganic and organic
ingredients free of contaminants and may also contain pure
protein additives, such as growth factors.
■ Their constituents are produced by genetic modification in
bacteria or yeast with the addition of vitamins, cholesterol,
particular amino acids, and fatty acids.

11. Protein free media:
■ Protein-free media is devoid of any protein and only
include non-protein constituents.
■ Usage of protein-free media enhances superior cell growth
and protein expression in contrast to serum-supplemented
media and enables downstream purification of any
expressed product.

12. REFERENCE LINK:
■ https://www.onlinebiologynotes.com/animal-cell-culture/
■ https://www.biotecharticles.com/Others-Article/Animal-Cell-
Culture-Media-Natural-and-Artificial-Media-376.html
■ https://www.labome.com/method/Cell-Culture-Media-A-
Review.html