3. 1-D NMR
• Simple FT-NMR Technique
• 1-D NMR spectroscopy is simple technique In which we
provide 90 degree pulse to sample which is placed in
uniform magnetic field. As a result we get a FID signal
(raw form). To get meaningful data we apply Fourier
Transform program on FID to get desire signal.
• Data in form of frequency vs intensity is plotted along
x-axis and y-axis respectively
5. Sequence Scheme
If we divide the 1-D NMR into a general periods , we get
two steps
•Preparation
•Detection
Preparation : (do something with nuclei)
What we can do ???
•Simply we provide a 90 degree pulse
Detection: ( detect the signal)
•Simply we measure the signal
7. Why Named 1-D NMR ??
• We call it 1-D NMR ,
not because of graph.
Graph is actually in 2-d.
• Since one of the time
domain is changing into
frequency dimension,
That’s why we call it
1D NMR,
Time dimension changing
into frequency dimension
8. 2-D NMR
• The first two-dimensional experiment was proposed by
Jean Jenner.
• 2D NMR is a set of nuclear magnetic resonance
spectroscopy (NMR) methods which give data plotted in
a space defined by two frequency axes rather than one.
• 2D NMR spectra provide more information about a
molecule than one-dimensional NMR spectra.
9. Principle
• 2D NMR is Nuclear magnetic resonance spectroscopy in
which we provide a 90 degree pulse to our sample which
is placed in uniform magnetic field then we wait for
(0ms-5ms) depending upon the experiment type then
again we provide again 90 degree pulse and this time we
measure signal as FID.
• As a result we get Two FID signals which are plotted
with respect to time domain.
• To get a meaningful data , we apply Fourier transform
program on FIDs to get frequency in terms of ppm.
10. 1. Primary 2D matrix consists a series of FIDs.
2. A set of 1D NMR spectra is obtained by Fourier transformation
with respect to t2
3. The signals of each transformation may differ in amplitude and
phase. A second Fourier transformation with respect to t1 yields
the final 2D matrix with frequency axes F1 and F2
1. 2. 3.
2D NMR
12. PreparationPreparation:: 90 degree pulse excite nuclei in sample.
EvolutionEvolution (t1): let them nuclei precess freely.
MixingMixing: again 90 degree r.f. pulses are applied and create
transverse magnetization
DetectionDetection (t2): observable transverse magnetization is recorded.
It is usually labeled with t2
Four periods in 2D experiment
20. Why 2D NMR
• As 1D NMR spectrum
of Macromolecules is
quite complex.
• So make this complex
spectrum easy, we use
2D NMR.
21. Types of 2D NMR
• Homonuclear Experiment : In this experiment
we provide two frequency to same nuclei in sample. i.e.
H-H Correlation spectroscopy (COSY) Actually in it we
basically see homonuclear connectivity between same
nuclei.
• Heteronuclear Experiment : In this experiment
wo provides two frequency to different nuclei in the
sample i.e. H-C Heteronuclear Correlation (HETCOR).