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ENAMEL 
Dr. Muznah Sultan 
Lecturer DIDC-DUHS 
Dental Anatomy
Physical Characteristics 
1. Forms a protective covering (2 mm – knife 
edge). 
2. Forms a resistant covering (suitable for 
mastication). 
3. The hardest calcified tissue in human body. 
4. Brittle. 
5. The specific gravity is 2.8. 
6. Acts as semipermeable membrane. 
7. Color: yellowish white to grayish white.
Tooth Layers 
longitudinal cross section of the Tooth showing : 
Enamel, Dentine, Pulp and Cementum
Chemical Properties 
• Inorganic materials (apatite crystals) 96% 
By weight 
• Organic substances and water 4% 
• In volume the organic matter and water 
are nearly equal to the inorganic 
contents.
Structure 
I. Prisms or rods. 
II. Rod sheath. 
III. Inter-prismatic substance. 
IV. Striations. 
V. Direction of rods. 
VI. Hunter-Schreger bands. 
VII. Incremental lines. 
VIII. Surface structures. 
IX. Enamel lamellae. 
X. Enamel tufts. 
XI. Dentino-enamel junction. 
XII. Odontoblastic processes and enamel spindles.
Enamel Rods or Prisms
Characteristics 
 Number: 5 – 12 millions. 
 Direction: Run in oblique direction and wavy 
course. 
 Length: greater than the thickness of E. 
 Diameter average: 4 μm. 
 Appearance: Have a clear crystalline 
appearance. 
 Cross-section: hexagonal, round, oval, or fish 
scales.
Enamel Rods
Submicroscopic 
Structure Of Enamel 
Rods 
 Keyhole or paddle-shaped. 
 Separated by interrod substance. 
 About 5 μm in breadth and 9 μm in length. 
 The bodies are near the occlusal or incisal 
surface. 
 The tails point cervically. 
 The crystals; parallel to the long axis of the 
prism heads. 
 Deviate about 65° from the tails.
Keyhole shaped E. rods 
Hexagonal ameloblasts 
Note crystal orientation 
Enamel Rod’s Shape
Crystals in rod and inter-rod enamel are similar in structure but diverge in 
orientation
Enamel Crystal 
 Crystals length: 0.05 – 1 μm. 
 Thickness: about 300 A°. 
 Average width: about 900 A°. 
 Cross sections: somewhat irregular.
Enamel Crystal 
Longitudinal Section Transverse Section
The Rod Sheath 
A thin peripheral layer. 
Darker than the rod. 
Relatively acid-resistant. 
Less calcified and contains more organic 
matter than the rod itself. 
Electron Microscope : often incomplete.
Inter-prismatic Substance 
•Cementing E. rods together. 
•More calcified than the rod sheath. 
•Less calcified than the rod itself. 
•Appears to be minimum in human teeth.
Cross-striations
Direction of Rods 
•Usually at right angles to the D. surface. 
•Follow a wavy course in clockwise and 
anticlockwise deviation. 
•At the cusps or incisal edges: gnarled 
enamel. 
•At pits and fissures: rods converge in their 
outward course.
Direction of Enamel Rods
Hunter-Schreger Bands 
•Alternating dark and light strips. 
•Have varying width. 
•Seen in large ground section (oblique 
reflected light). 
•Originate from the DEJ.
Hunter-Schreger Bands
Hunter-Schreger Bands
Hunter-Schreger Bands 
This is Due to: 
1. Change in the direction of E. rods. 
2. Variation in calcification of the E. 
3. Alternate zones having different permeability 
and organic material. 
4. Optical phenomenon.
Incremental Lines 
A. Incremental Lines of Retzius 
B. Neonatal Line
Incremental Lines of Retzius: 
 Brownish bands in ground sections. 
 Reflect variation in structure and mineralization. 
 Broadening of these lines occur in metabolic 
disturbances. 
 Etiology 
1. Periodic bending of E. rods. 
2. Variation in organic structure. 
3. Physiologic calcification rhythm.
Incremental Lines of Retzius:
Neonatal Line 
 The E. of the deciduous teeth and the 1st 
permanent molar develop partly before birth 
and partly after birth, the boundary between 
both is marked by neonatal line or ring. 
 Etioloyg 
 Due to sudden change in the environment and 
nutrition. 
 The antenatal E. is better calcified than the 
postnatal E.
Neonatal Line
SURFACE 
STRUCTURES
Surface Structures 
a. Structureless layer (E. skin) 
b. Perikymata 
c. Rod ends 
d. Cracks
a. Structureless layer 
 About 30 μm thick. 
 In 70% permanent teeth and all deciduous teeth. 
 Found least often over the cusp tips. 
 Found commonly in the cervical areas. 
 No E. prisms. 
 All the apatite crystals area parallel to one another and 
perpendicular to the striae of Retzius. 
 More mineralized than the bulk of E. beneath it.
b. Perikymata 
 Transverse wave like grooves. 
 Thought to be the external manifestation of the striae of Retzius. 
 Lie parallel to each other and to CEJ. 
 Number: 
 About 30 perik./mm at the CEJ. 
 About 10 perik./mm near the incisal edge. 
 Their course is regular, but in the cervical region, it may be 
quite irregular. 
 Powdered graphite demonstrates them. 
 It is absent in the occlusal part of deciduous teeth but 
present in postnatal cervical part (due to undisturbed and 
even development of E. before birth)
The relationship between the striae 
of Retziuz and surface perikymata 
Striae of Retziuz Perikymata
c. Rod ends 
 Are concave and vary in depth and shape. 
 Are shallow in the cervical regions. 
 Deep near the incisal or occlusal edges.
Rod ends
d. Cracks 
 Narrow fissure like structure. 
 Seen on almost all surfaces. 
 They are the outer edges of lamellae. 
 Extend for varying distance along the surface. 
 At right angles to CEJ. 
 Long cracks are thicker than the short one. 
 May reach the occlusal or incisal edge.
Cracks
ENAMEL LAMELLAE
Enamel Lamellae 
 Are thin, leaf like structures, 
 Develop in planes of tension. 
 Extends from E. surface towards the DEJ. 
 Confused with cracks caused by grinding 
(decalcification). 
 Extend in longitudinal and radial direction. 
 Represent site of weakness in the tooth and 
three types; A, B, and C.
Enamel Lamellae
Enamel Lamellae
ENAMEL TUFTS
Enamel Tufts 
 Arise from DEJ. 
 Reach to 1/5 – 1/3 the thickness of E. 
 In ground section: resemble tufts of grass. 
 Do not spring from a single small area. 
 The inner end arises at the dentin. 
 Consist of hypocalcified E. rods and 
interprismatic substance. 
 The extend in the direction of the long axis of 
the crown (best seen in horizontal sections).
Enamel Tufts
Enamel Tufts
DENTINO-ENAMEL 
JUNCTION
Dentino-Enamel Junction 
 Scalloped junction – the convexities towards 
D. 
 At this junction, the pitted D. surface fit 
rounded projections of the enamel. 
 The outline of the junction is performed by the 
arrangement of the ameloblasts and the B. M.
Dentino-Enamel Junction
ODONTOBLASTIC 
PROCESSES AND 
ENAMEL SPINDLES
Odontoblastic Processes and 
Enamel Spindles 
 The odontoblasts processes may cross DEJ 
(before the hard substance is formed) to the E. 
and ends as E. spindles. 
 They are filled with organic matter. 
 The processes and spindles are at right angle to 
the surface of the dentin. 
 The direction of spindles and rods is divergent. 
 Spindles appear dark in ground sections under 
transmitted light.
Odontoblastic Processes and 
Enamel Spindles
Abnormalities 
 Interference during E. matrix formation may 
cause Enamel hypoplasia. 
 Interference during Enamel maturation may 
cause Enamel hypocalcification. 
 Each condition may be caused by systemic, 
local, or hereditary factors.
Abnormalities 
Enamel Hypocalcification Enamel Hypoplasia
Thank you!!

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Enamel - DCPS

  • 1. ENAMEL Dr. Muznah Sultan Lecturer DIDC-DUHS Dental Anatomy
  • 2. Physical Characteristics 1. Forms a protective covering (2 mm – knife edge). 2. Forms a resistant covering (suitable for mastication). 3. The hardest calcified tissue in human body. 4. Brittle. 5. The specific gravity is 2.8. 6. Acts as semipermeable membrane. 7. Color: yellowish white to grayish white.
  • 3. Tooth Layers longitudinal cross section of the Tooth showing : Enamel, Dentine, Pulp and Cementum
  • 4. Chemical Properties • Inorganic materials (apatite crystals) 96% By weight • Organic substances and water 4% • In volume the organic matter and water are nearly equal to the inorganic contents.
  • 5. Structure I. Prisms or rods. II. Rod sheath. III. Inter-prismatic substance. IV. Striations. V. Direction of rods. VI. Hunter-Schreger bands. VII. Incremental lines. VIII. Surface structures. IX. Enamel lamellae. X. Enamel tufts. XI. Dentino-enamel junction. XII. Odontoblastic processes and enamel spindles.
  • 6. Enamel Rods or Prisms
  • 7. Characteristics  Number: 5 – 12 millions.  Direction: Run in oblique direction and wavy course.  Length: greater than the thickness of E.  Diameter average: 4 μm.  Appearance: Have a clear crystalline appearance.  Cross-section: hexagonal, round, oval, or fish scales.
  • 9. Submicroscopic Structure Of Enamel Rods  Keyhole or paddle-shaped.  Separated by interrod substance.  About 5 μm in breadth and 9 μm in length.  The bodies are near the occlusal or incisal surface.  The tails point cervically.  The crystals; parallel to the long axis of the prism heads.  Deviate about 65° from the tails.
  • 10. Keyhole shaped E. rods Hexagonal ameloblasts Note crystal orientation Enamel Rod’s Shape
  • 11. Crystals in rod and inter-rod enamel are similar in structure but diverge in orientation
  • 12. Enamel Crystal  Crystals length: 0.05 – 1 μm.  Thickness: about 300 A°.  Average width: about 900 A°.  Cross sections: somewhat irregular.
  • 13. Enamel Crystal Longitudinal Section Transverse Section
  • 14. The Rod Sheath A thin peripheral layer. Darker than the rod. Relatively acid-resistant. Less calcified and contains more organic matter than the rod itself. Electron Microscope : often incomplete.
  • 15. Inter-prismatic Substance •Cementing E. rods together. •More calcified than the rod sheath. •Less calcified than the rod itself. •Appears to be minimum in human teeth.
  • 17. Direction of Rods •Usually at right angles to the D. surface. •Follow a wavy course in clockwise and anticlockwise deviation. •At the cusps or incisal edges: gnarled enamel. •At pits and fissures: rods converge in their outward course.
  • 19. Hunter-Schreger Bands •Alternating dark and light strips. •Have varying width. •Seen in large ground section (oblique reflected light). •Originate from the DEJ.
  • 22. Hunter-Schreger Bands This is Due to: 1. Change in the direction of E. rods. 2. Variation in calcification of the E. 3. Alternate zones having different permeability and organic material. 4. Optical phenomenon.
  • 23. Incremental Lines A. Incremental Lines of Retzius B. Neonatal Line
  • 24. Incremental Lines of Retzius:  Brownish bands in ground sections.  Reflect variation in structure and mineralization.  Broadening of these lines occur in metabolic disturbances.  Etiology 1. Periodic bending of E. rods. 2. Variation in organic structure. 3. Physiologic calcification rhythm.
  • 26. Neonatal Line  The E. of the deciduous teeth and the 1st permanent molar develop partly before birth and partly after birth, the boundary between both is marked by neonatal line or ring.  Etioloyg  Due to sudden change in the environment and nutrition.  The antenatal E. is better calcified than the postnatal E.
  • 29. Surface Structures a. Structureless layer (E. skin) b. Perikymata c. Rod ends d. Cracks
  • 30. a. Structureless layer  About 30 μm thick.  In 70% permanent teeth and all deciduous teeth.  Found least often over the cusp tips.  Found commonly in the cervical areas.  No E. prisms.  All the apatite crystals area parallel to one another and perpendicular to the striae of Retzius.  More mineralized than the bulk of E. beneath it.
  • 31. b. Perikymata  Transverse wave like grooves.  Thought to be the external manifestation of the striae of Retzius.  Lie parallel to each other and to CEJ.  Number:  About 30 perik./mm at the CEJ.  About 10 perik./mm near the incisal edge.  Their course is regular, but in the cervical region, it may be quite irregular.  Powdered graphite demonstrates them.  It is absent in the occlusal part of deciduous teeth but present in postnatal cervical part (due to undisturbed and even development of E. before birth)
  • 32. The relationship between the striae of Retziuz and surface perikymata Striae of Retziuz Perikymata
  • 33. c. Rod ends  Are concave and vary in depth and shape.  Are shallow in the cervical regions.  Deep near the incisal or occlusal edges.
  • 35. d. Cracks  Narrow fissure like structure.  Seen on almost all surfaces.  They are the outer edges of lamellae.  Extend for varying distance along the surface.  At right angles to CEJ.  Long cracks are thicker than the short one.  May reach the occlusal or incisal edge.
  • 38. Enamel Lamellae  Are thin, leaf like structures,  Develop in planes of tension.  Extends from E. surface towards the DEJ.  Confused with cracks caused by grinding (decalcification).  Extend in longitudinal and radial direction.  Represent site of weakness in the tooth and three types; A, B, and C.
  • 42. Enamel Tufts  Arise from DEJ.  Reach to 1/5 – 1/3 the thickness of E.  In ground section: resemble tufts of grass.  Do not spring from a single small area.  The inner end arises at the dentin.  Consist of hypocalcified E. rods and interprismatic substance.  The extend in the direction of the long axis of the crown (best seen in horizontal sections).
  • 46. Dentino-Enamel Junction  Scalloped junction – the convexities towards D.  At this junction, the pitted D. surface fit rounded projections of the enamel.  The outline of the junction is performed by the arrangement of the ameloblasts and the B. M.
  • 48. ODONTOBLASTIC PROCESSES AND ENAMEL SPINDLES
  • 49. Odontoblastic Processes and Enamel Spindles  The odontoblasts processes may cross DEJ (before the hard substance is formed) to the E. and ends as E. spindles.  They are filled with organic matter.  The processes and spindles are at right angle to the surface of the dentin.  The direction of spindles and rods is divergent.  Spindles appear dark in ground sections under transmitted light.
  • 50. Odontoblastic Processes and Enamel Spindles
  • 51. Abnormalities  Interference during E. matrix formation may cause Enamel hypoplasia.  Interference during Enamel maturation may cause Enamel hypocalcification.  Each condition may be caused by systemic, local, or hereditary factors.