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Dentin 
Dr. Muznah Sultan 
Lecturer DIDC, DUHS
1. Introduction and Chemical 
Composition 
 Dentin is a hard bone-like tissue that is 
present in the crown as well as in the 
root of teeth. 
 In the crown, dentin is covered by 
enamel and in the root it is covered by 
cementum. 
 Unlike enamel, dentin is a vital tissue 
containing the cell processes of 
odontoblasts and neuron. 
 Mature dentin has 20% organic, 70% 
inorganic and 10% water by weight .
 The organic matrix of dentin is collagenous 
 It provides resiliency to the crown which is 
necessary to withstand the forces of 
mastication 
 The principle inorganic component of 
dentin is hydroxyapatite crystals 
 The high mineral content of dentin 
makes it harder than bone and 
cementum but softer than enamel
2. Dentinal structure and 
classification 
 Dentin is packed with dentinal tubules that 
traverse its entire thickness 
 Dentinal tubules contain odontoblastic 
process and nerve fibres 
 The odontoblastic processes have numerous 
side branches that exist in the lateral 
branches of the dentinal tubules
Odontoblasts – peri-tubular and inter-tubular 
dentin has been removed 
Dentine tubules – odontoblastic 
processes removed
Odontoblastic process
3. Histological Features of Dentin 
Depending on its time of development Dentin is classified as: 
Primary dentin 
Secondary dentin 
Tertiary dentin
Primary dentin 
Primary dentin is the developmental dentin that is formed 
before and during eruption 
Secondary dentin 
Secondary dentine develops after the root formation has 
been completed 
Deposition of secondary dentin is continuous but much 
slower
Dr.Syed Sadatullah 
Types of Dentin
Tertiary dentin 
Tertiary dentin is produced in reaction to stimulus, such as 
attrition , caries or restorative dental procedure 
Tertiary dentin can be reactionary or reparative 
Reactionary dentin is that type of tertiary dentin that is 
deposited by the pre-existing odontoblasts 
Reparative dentin is deposited by newly differentiated 
odontoblasts. Irregular dentin formed in response to an 
irritant.
Dr.Syed Sadatullah 
Secondary and Tertiary Dentin
Dr.Syed Sadatullah 
Types of Dentin 
Predentin 
Mantle Dentin 
Circumpulpal Dentin 
Inter-globular Dentin 
Sclerotic Dentin 
Intra-tubular Dentin 
Inter-tubular Dentin 
Tomes Granular layer
Dr.Syed Sadatullah 
Odontoblast process in dentin tubule
Predentin 
Predentin is the first deposited layer of un-mineralized 
matrix of dentin 
Mantle Dentin 
The outer layer of dentine that mineralizes first is called 
mantle dentin. It lies near the DEJ 
Circumpulpal Dentin 
The bulk of dentin underlying the mantle dentin is called 
circumpulpal 
Dr.Syed Sadatullah
Dr.Syed Sadatullah 
Mantle Dentin – Inter-globular Dentin – Circumpulpal Dentin
Inter-globular Dentin 
Dentine separating the mantle dentin and circumpulpal 
dentin is hypo-mineralized and is called inter-globular 
dentin 
Interglobular Dentin 
Dr.Syed Sadatullah
Intra-tubular Dentin 
Dentin surrounding and nearest to each tubule is hyper-mineralized 
and is called intra-tubular dentin or peri-tubular 
dentin 
If there is increased mineralization of intra-tubular 
dentine the tubule gets occluded and the resulting dentine 
is called sclerotic dentin or transparent dentin 
If odontoblasts die as a result of injury or old age the 
tubule are referred to as dead tracts 
Dr.Syed Sadatullah
Dr.Syed Sadatullah 
Intra-tubular Dentin
Inter-tubular Dentin 
The dentinal matrix that lies between the intra-tubular 
dentin is inter-tubular dentin 
Dr.Syed Sadatullah 
Inter-tubular Dentin
Tomes granular layer 
In the roots near the cemento-dentinal junction there are 
hypo-mineralized areas of dentin around the dentinal 
tubule called the Tomes granular layer 
Dr.Syed Sadatullah
4. Histological Features of Dentin 
Incremental growth line 
The organic matrix of dentine is deposited incrementally at a daily 
rate of 4μm 
At the boundary of each daily increment, minute changes in 
collagen fibre orientation is observed which manifest as 
incremental lines 
Incremental lines run perpendicular to the dentinal tubules 
Dr.Syed Sadatullah
Dr.Syed Sadatullah 
Incremental lines
Dr.Syed Sadatullah 
Incremental lines
Incremental lines of von Ebner 
In ground section the 5-day increment can be seen as 
incremental or imbrications' lines of von Ebner 
Counter lines Owen 
Counter lines of Owen represent normal physiological 
alterations in the pattern of mineralization 
Neonatal line represents an exaggerated counter line of 
Owen. 
Neonatal lines are seen in all primary teeth and the first 
permanent molars 
Dr.Syed Sadatullah
Dr.Syed Sadatullah 
Lines of Owen 
Neonatal Line in Enamel and Dentin
Thank You!! 
Dr.Syed Sadatullah Ibn Sina National Medical College

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Dentin.dcp

  • 1. Dentin Dr. Muznah Sultan Lecturer DIDC, DUHS
  • 2. 1. Introduction and Chemical Composition  Dentin is a hard bone-like tissue that is present in the crown as well as in the root of teeth.  In the crown, dentin is covered by enamel and in the root it is covered by cementum.  Unlike enamel, dentin is a vital tissue containing the cell processes of odontoblasts and neuron.  Mature dentin has 20% organic, 70% inorganic and 10% water by weight .
  • 3.  The organic matrix of dentin is collagenous  It provides resiliency to the crown which is necessary to withstand the forces of mastication  The principle inorganic component of dentin is hydroxyapatite crystals  The high mineral content of dentin makes it harder than bone and cementum but softer than enamel
  • 4. 2. Dentinal structure and classification  Dentin is packed with dentinal tubules that traverse its entire thickness  Dentinal tubules contain odontoblastic process and nerve fibres  The odontoblastic processes have numerous side branches that exist in the lateral branches of the dentinal tubules
  • 5. Odontoblasts – peri-tubular and inter-tubular dentin has been removed Dentine tubules – odontoblastic processes removed
  • 7. 3. Histological Features of Dentin Depending on its time of development Dentin is classified as: Primary dentin Secondary dentin Tertiary dentin
  • 8. Primary dentin Primary dentin is the developmental dentin that is formed before and during eruption Secondary dentin Secondary dentine develops after the root formation has been completed Deposition of secondary dentin is continuous but much slower
  • 10. Tertiary dentin Tertiary dentin is produced in reaction to stimulus, such as attrition , caries or restorative dental procedure Tertiary dentin can be reactionary or reparative Reactionary dentin is that type of tertiary dentin that is deposited by the pre-existing odontoblasts Reparative dentin is deposited by newly differentiated odontoblasts. Irregular dentin formed in response to an irritant.
  • 11. Dr.Syed Sadatullah Secondary and Tertiary Dentin
  • 12. Dr.Syed Sadatullah Types of Dentin Predentin Mantle Dentin Circumpulpal Dentin Inter-globular Dentin Sclerotic Dentin Intra-tubular Dentin Inter-tubular Dentin Tomes Granular layer
  • 13. Dr.Syed Sadatullah Odontoblast process in dentin tubule
  • 14. Predentin Predentin is the first deposited layer of un-mineralized matrix of dentin Mantle Dentin The outer layer of dentine that mineralizes first is called mantle dentin. It lies near the DEJ Circumpulpal Dentin The bulk of dentin underlying the mantle dentin is called circumpulpal Dr.Syed Sadatullah
  • 15. Dr.Syed Sadatullah Mantle Dentin – Inter-globular Dentin – Circumpulpal Dentin
  • 16. Inter-globular Dentin Dentine separating the mantle dentin and circumpulpal dentin is hypo-mineralized and is called inter-globular dentin Interglobular Dentin Dr.Syed Sadatullah
  • 17. Intra-tubular Dentin Dentin surrounding and nearest to each tubule is hyper-mineralized and is called intra-tubular dentin or peri-tubular dentin If there is increased mineralization of intra-tubular dentine the tubule gets occluded and the resulting dentine is called sclerotic dentin or transparent dentin If odontoblasts die as a result of injury or old age the tubule are referred to as dead tracts Dr.Syed Sadatullah
  • 19. Inter-tubular Dentin The dentinal matrix that lies between the intra-tubular dentin is inter-tubular dentin Dr.Syed Sadatullah Inter-tubular Dentin
  • 20. Tomes granular layer In the roots near the cemento-dentinal junction there are hypo-mineralized areas of dentin around the dentinal tubule called the Tomes granular layer Dr.Syed Sadatullah
  • 21. 4. Histological Features of Dentin Incremental growth line The organic matrix of dentine is deposited incrementally at a daily rate of 4μm At the boundary of each daily increment, minute changes in collagen fibre orientation is observed which manifest as incremental lines Incremental lines run perpendicular to the dentinal tubules Dr.Syed Sadatullah
  • 24. Incremental lines of von Ebner In ground section the 5-day increment can be seen as incremental or imbrications' lines of von Ebner Counter lines Owen Counter lines of Owen represent normal physiological alterations in the pattern of mineralization Neonatal line represents an exaggerated counter line of Owen. Neonatal lines are seen in all primary teeth and the first permanent molars Dr.Syed Sadatullah
  • 25. Dr.Syed Sadatullah Lines of Owen Neonatal Line in Enamel and Dentin
  • 26. Thank You!! Dr.Syed Sadatullah Ibn Sina National Medical College