molecular approaches

1. BREEDING RICE FOR DROUGHT
TOLERANCE
B.MOHANAPRIYA
2017600808
A green revolution
to
gene revolution

3. Drought - complex trait

4. CLASSIFICATION OF DROUGHT

5. Early drought Intermittent drought
Terminal drought

6. Severity Index (source: Aigup Dai, Wiley interdisciplinary Reviews: Climate Change; july 2012)

10. Conventional
breeding approach

13. DTY Qtls
• IRRI developed 50 mapping population.
• 14 QTLs, in which a single QTL explained 9-39% of
the phenotypic variation.
• These QTLs distributed on eight chromosomes.

15. qDTY12.1 a large effect qtl
• qDTY12.1 works as a gene-complex of 10
genes
• transcription factor OsNAM is a major
regulator.

16. candidate gene analysis has been carried out in this QTL region and several genes have been
reported as putative candidate genes, including a GRAM-domain containing protein, an
Amydohydrolase, a Nodulin MtN3, a No Apical Meristem, a Cellulose Synthase A
(CesA10) and a cytochrome P450 associated with different processes such as root hair
proliferation/elongation, pollen fertility, cell wall permeability and signal transduction

18. Pleiotropy vs Linkage

21. qDTY1.1, qDTY2.2, qDTY3.1, qDTY3.2, qDTY6.1, and qDTY12.1
showed an effect against two or more high-yielding genetic
backgrounds in both the lowland and upland ecosystem, indicating
their usefulness in increasing the grain yield of rice under drought

22. Secondary traits

25. REFERENCES
• K.S. Fischer, R. Lafitte, S. Fukai,G. Atlin, and B. Hardy , Breeding Rice for
Drought-Prone Environments ,IRRI,2003
• Nitika Sandhu and Arvind Kumar , Bridging the Rice Yield Gaps under Drought:
QTLs,Genes, and Their Use in Breeding Programs ,MDPI.2017.
• Vikram et al, qDTY1.1, a major QTL for rice grain yield under reproductive-
stage drought stress with a consistent effect in multiple elite genetic
backgrounds. BMC Genetics 2011, 12:89
• Mishra et al, qDTY12.1: a locus with a consistent effect on grain yield under
drought in rice. BMC Genetics 2013, 14:12

26. Thank you