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Dr Pradeep Kumar
Professor, Physiology
KGMU
The Plasma Membrane – a Phospholipid
Bilayer
Components of Plasma Membrane
Lipids Proteins
1) Phospholipids
2) Cholesterol
3) Glycolipds
1) Peripheral
(Associated)
2) Integral
(Membrane Spanning)
3) Glycoproteins
~50% ~50%
(75%)
(20%)
(5%)
 Isolate the cell’s contents from the external envi
ronment
 Regulate traffic in and out of the cell
 Communicate with other cells
The phospholipid bilayer
1. Impermeable to water-soluble and polar molecules, ions
2. Permeable to small and nonpolar molecules
3. Lipids oriented with polar heads facing out
tails
(hydrophobic)
head
(hydrophilic)
hydrophobic
tails
hydrophilic
heads
hydrophilic
heads
extracellular fluid
(watery environment)
cytoplasm
(watery environment)
phospholipid
bilayer
 Membranes are “fluid mosaics” with proteins e
mbedded in or attached to the membrane
 Proteins can move within the fluid lipid bilayer
extracellular fluid (outside)
cytoplasm (inside)
protein filaments
recognition protein receptor protein transport protein
binding site
phospholipid
bilayer
phospholipid cholesterol
carbohydrate
1. Transport proteins
◦ regulate the movement of water-soluble molecul
es across the membrane
 Ion Channel proteins, Pumps, Receptors
 Carrier proteins, Enzymes, Cell adhesion Mol
ecules
2. Receptor Proteins
◦ trigger cellular response when specific mo
lecules bind to them
Nervous system
Endocrine system
 Passive transport is a function of molecular si
ze, lipid solubility, and size of the concentrati
on gradient
1. Simple diffusion
1 A drop of dye is
placed in water.
2 Dye molecules
diffuse into the
water; water
molecules diffuse
into the dye.
drop of dye
pure water
3 Both dye molecules
and water molecules
are evenly dispersed.
(a) simple diffusion
(extracellular fluid)
(cytoplasm)
Passive transport…(cont.)
◦ 2. Osmosis
• a. Isotonic
• b. Hypertonic
• c. Hypotonic
(a) isotonic solution (b) hypertonic solution (c) hypotonic solution
10 micrometers
equal movement of water
into and out of cells
net water movement
out of cells
net water movement
into cells
Passive transport…(cont.)
◦ 3. Facilitated diffusion
ions
(b) facilitated diffusion through a channel
channel
protein
proteins forming
permanent
hydrophilic channel
(extracellular fluid)
(cytoplasm)
Carrier protein
has binding sit
e for molecule
.
amino acids,
sugars,
small proteins
(c) facilitated diffusion through a carrier
carrier
protein
Molecule enter
s binding site.
Carrier protein changes
shape, transporting molecule
across membrane.
Carrier protein res
mes original shap
Energy-requiring transport
1. Active transport
• Ion gradients and energy production
2. Endocytosis
3. Exocytosis
Primary Active Transport: The Na+/K+ Pump
Antiport
Secondary Active
(Indirect):
e.g.,
Na+/Glucose transporter
Symport
(extracellular fluid)
Transport protein
binds ATP and Ca
2+.
Transport protein uses e
nergy from ATP to chan
ge shape and move
ion across membrane.
Transport protein
resumes original
shape.
(cytoplasm)
transport protein
ATP bin
ding sit
e
recognition
site
ATP
Ca2+
(extracellular fluid)
(cytoplasm)
food particle
particle
enclosed in vesicle
phagocytosis
vesicle containing
extracellular
fluid
cell
pseudopod
pinocytosis
(a)
(b)
1
3
3
2
1 3
2
Thank You

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