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CURRICULUM VITAE
I Personal
Name: Marines Victoria Longart Marcano
Citizenship: Venezuelan
Current Position
Principal Investigator: August 2005-Present
Neuroscience Unit, Instituto de Estudios Avanzados-IDEA. Carretera Nacional Hoyo de la Puerta,
Valle de Sartenejas-Baruta. Caracas, Edo. Miranda, 1080. Venezuela. Phone: 58-212-9035191.
II. Education
1994-1999: Graduate Studies in Biological Sciences, Department of Cell Biology, Universidad
Simón Bolívar (USB). Caracas, Venezuela. Doctor in Biological Sciences (Ph.D), 1999
1987- 1994: Undergraduate studies. School of Sciences: Department of Biology, Universidad de
Oriente (UDO). Cumaná, Venezuela. Licentiate in Biology (BS), 1994
Other Courses
1) 1st Meeting on Rare Diseases. Fundación Instituto de Estudios Avanzados (IDEA). Caracas,
March, 2012. Venezuela.
2) Study of Proteins and Antibodies for Biotechnology and Biomedicine. Instituto de Estudios
Avanzados-IDEA. Caracas, Venezuela. November 23-27, 2009.
3) Confocal Microscopy Course, workshop for users of Carl Zeiss systems. Instituto de Estudios
Avanzados-IDEA. Caracas, Venezuela. May 5-14, 2008.
4) Proteomic Approaches in Molecular Biology: Theory and Practice. Universidad de Buenos Aires.
Argentina (co-organized by ICGEB). March 3-14, 2008.
5) Isolation, Establishment and Maintenance of Neuronal Stem Cells of the Postnatal adult Brain.
Center for Neuroscience Research. Children´s Nacional Medical Center. Washington, December 6-7
United States.
6) Immunocytochemistry, In Situ Hybridization and Live Cell Imaging. Cold Spring Harbor
Laboratory. New York, USA. October 18-31, 2003.
III. Research and Professional Experience
1) Principal Investigator, Instituto de Estudios Avanzados-IDEA. August 2005-present.
2) Visiting Fellow, National Institutes of Health (NIH), Bethesda, MD, USA. August 2000- July
2005 (Supervisor: Dr. Andres Buonanno).
3) Postdoctoral Researcher, Instituto de Estudios Avanzados- IDEA, Caracas, Venezuela 1999-
2000.
4) Doctoral Student, Instituto de Estudios Avanzados-IDEA, Caracas, Venezuela 1995-1999.
5) Research Assistant. Genetics of Yeast, Centro Tecnológico Polar. Caracas, Venezuela, 1995.
6) Teaching and Research Assistant. Photosynthetic Metabolism. Universidad Simon Bolivar
(USB), Caracas, Venezuela, 1994-1995.
IV. Scientific Publications
Articles in Scientific Journals
1) Bonetti V, Carballo J, Garcia L, Longart M (2016). Nav1.7 Sodium Channel Isoform is
Expressed in Growth Cones of Hippocampal Neurons in Culture. Under review at Epilepsy Journal
(Corresponding author).
2) Sanchez-Mendoza E, Carballo J, Longart M, Hermann D, Doeppner T (2016). Implantation of
Miniosmotic Pumps and Delivery of Tract Tracers to Study Brain Reorganization in
Pathophysiological Conditions. J Vis Exp. Jan 18; (107). doi: 10.3791/52932.
3) Trigos A, Longart M, García L, Castillo C, Forsyth P, Medina R (2015). ProNGF derived from
rat sciatic nerves downregulates neurite elongation and axon specification in PC12 cells. Front. Cell.
Neurosci. 9:364. doi: 10.3389/fncel.2015.00364.
4) García L, Castillo C, Carballo J, Rodríguez Y, Forsyth P, Medina R, Martínez JC, Longart M
(2013). ErbB Receptors and PKC Regulate PC12 Neuronal-like Differentiation and Sodium Current
Elicitation. Neuroscience. 236: 88-98. (Corresponding author).
5) Carballo J, Sánchez F, Núñez J, Castillo C, García L, Longart M (2012). Efecto del Medio
Condicionado por Nervio Ciático en la Angiogénesis de Axones Periféricos Regenerados Mediante
Tubulización. (Effect of Sciateic Nerve Conditioned Medium in Angiogenesis of Peripheral Axons
Regenerated by Tubulization). Acta Microscópica. Vol 21, supp 2: 87-88.
6) Garcia L, Castillo C, Longart M (2012). Factores Liberados por Nervios Ciáticos Afectan la
Expresión de Neurofilamantos H (Factors released by Sciatic Nerves Affect the Expresión of
Neurofilaments H). Acta Microscópica. Vol 21, supp B: 89-90.
7) Carballo J, Sánchez F, Núñez J, Castillo C, Longart M (2011). Efecto del Medio Condicionado
Proveniente del Nervio Ciático de Rata sobre la Regeneración y Re-mielinización de Axones
Periféricos. (Effect of Rat Sciatic Nerve Conditioned Medium on Regeneration and Remielinization
of Peripheral Axons). Acta Microscópica. Vol 20, supp B.
8) Correa G, Longart M (2010). Morphometric Analysis of Differentiation Process of Hippocampal
Neurons (Análisis Morfométrico del Proceso de Diferenciación in vitro de Neuronas del
Hipocampo). Investigación Clínica. 51 (4): 501-518. (Corresponding author).
9) Franco A Y, Longart M (2009). Apliccations of Green Fluorescent Protein (GFP) in Cell Biology
and Visualization of the Nervous System (Aplicaciones de la Proteína Verde Fluorescente (GFP) en
la Biología Celular y en la Visualización del Sistema Nervioso). Revista de Estudios
Transdisciplinarios RET. Fundación Instituto de Estudios Avanzados IDEA. 1 (2): 83-94 (Jul-Dec
2009). (Corresponding author).
10) Longart M, García L, Castillo C, Martínez JC, Medina R, Forsyth P, Malavé C (2009). Sciatic
Nerve Conditioned Medium Depleted of Pro-NGF Modulates Sodium Currents and Neurite
Outgrowth in PC12 Cells. Neuroscience, 159: 550-558. (Corresponding author).
11) Longart M, Chatani-Hinze M, Gonzalez C, Vullhorst D, Buonanno A. (2007). Regulation of
ErbB-4 endocytosis by Neuregulin in cultured GABAergic hippocampal interneurons. Brain Res
Bull. 73(4-6): 210-219.
12) Buonanno A, Kwon O-B, Vullhorst D, Hoffman D, Longart M. (2005). Regulation of synaptic
plasticity by the NRG-1/ErbB signaling pathway: Possible implications for
schizophrenia. Neuropsychopharmacology. 30: S70-S70 Suppl.
13) Kwon O-B*, Longart M*, Vullhorst D, Hoffman D, Buonanno A. (2005). Neuregulin reverses
long-term potentiation at CA1 hippocampal synapses. J. of Neuroscience, 25 (41): 9378-9383
(*equal contribution).
14) Longart M, Liu Y, Karavanova I, Buonanno A (2004). Neuregulin-2 is developmentally
regulated and targeted to dendrites of central neurons. J Comp Neurol. 472: 156-172.
15) Longart M, Buonanno A (2002). Neuregulins: A family of factors with critical functions during
nervous system development and in the cellular transformation and differentiation (Las
Neuregulinas: una familia de factores con funciones críticas en el desarrollo del sistema nervioso, y
en la diferenciación y transformación celular) Revista de Neurología, 34:91-97.
16) Villegas, R, Villegas G M, Longart M, Hernández M, Maqueira B, Buonnano A, García R,
Castillo C (2000). Neuregulin found in cultured-sciatic nerve conditioned medium causes neuronal
differentiation of PC12. Brain Res. 852 (2): 305-318.
17) Castrillo M, Aso P, Longart M, Vermehren A (1997). In situ inmunofluorescent of Ribulose-1,5-
bisphosphate carboxilase/ oxigenase in mesophyl of C-4 dicotyledonous plants. Photosynthetica 33
(1): 39-50.
Chapters in Book
1) Andrés Buonanno, Oh-Bin Kwon, Leqin Yan, Carmen Gonzalez, Marines Longart, Dax Hoffman
and Detlef Vullhorst (2008). Neuregulins and neuronal plasticity: possible relevance in
schizophrenia. Wiley, Novartis Foundation. Symposium 289: Growth Factors and Psychiatric
Disorders. Pages: 165-179. ISBN: 978-0-470-51604-1. Chapter in Book
Articles in preparation
1) García L, Castillo C, Correa G, Hernandez M, Longart M (2016). Proteins from Sciatic Nerve
Conditioned Medium Influence Glia and Neuronal Hippocampal Cell Fates. (Corresponding author).
2) García L, Castillo C, Hernandez M, Longart M (2016). Sciatic Nerve Conditioned Medium
inhibits sodium current induction and Neurofilament-H expression in GABAergic hippocampal
neurons (Corresponding author).
V. Awards, honors and fellowships
1) Fellowship from the DAAD in the Program Research Stays for University Academics and
Scientists (for assistant teachers, assistant professors and lecturers) to work at the Unit of Vascular
Neurology, University Hospital Essen. May-August 2016.
2) Member of the Program for Research Support, Level B. National Observatory of Science and
Technology (ONCTI). Venezuelan Ministry of Science and Technology (2011-2013; 2013-present).
3) Grant to perform the Workshop: Strengthening Project in Strategic Networks and New Modes of
Scientific Production and Technology Transfer. Fundacion Instituto de Estudios Avanzados-IDEA.
Caracas, 20 and 21 June 2013. Venezuela. Grant FONACIT No PC: 201302199. Venezuelan
Ministry of Science and Technology.
4) Member of the Editorial Board of the Journal: Chinese Journal of Clinicians, 2011-2014.
5) Fellowship to attend the Course "Proteomic Approaches in Molecular Biology" held in Argentina,
03-14 March 2008. FONACIT No. S3-2008000130. Venezuelan Ministry of Science and
Technology.
6) Member of research promotion program (PPI), (1997-2002; 2005-2010). National Observatory of
Science and Technology (ONCTI), Venezuelan Ministry of Science and Technology.
7) Postdoctoral Fellowship from the National Institutes of Health, August 2000- July 2005.
8) Selection of the publication: “Neuregulin-2 is developmentally regulated and targeted to dendrites
of central neurons”. J Comp Neurol. 472: 156-172 (2004), for the journal commentaries Title:
Multiple Personalities of Neuregulin Gene Family. Journal of Comparative Neurology 472: 134-139
(2004) by David A. Talmage and Lorna W. Role.
9) Fellowship to perform Graduate Studies, National Research Council (CONICIT) Venezuela,
1995-1999.
VI. Research Grants
1) Fellowship from the DAAD within the Program Research Stays for University Academics and
Scientists (for assistant teachers, assistant professors and lecturers) to work at the Unit of Vascular
Neurology, University Hospital Essen. Project: Modulating Neuregulin-1 to stimulate neurite
plasticity after in vitro neuronal injury. Objective: In vitro studies of molecules involved in the
morphology and functioning of neurons to identify potential therapeutic agents for neuronal
differentiation and regeneration. Funding: Monthly stipend, travel allowance and health insurance
are sponsored by DAAD. Consumables, equipments and reagents provided by he host laboratory.
Role: Investigator Dates: May-August 2016
2) Project: Role of neurotropich factors contained in a sciatic nerve conditioned medium in neuronal
differentiation. Objective: Study of molecules involved in the morphology and functioning of neurons
to identify potential therapeutic agents for neuronal differentiation and regeneration. Note: Within this
project I have a current collaboration with Dr. Dirk Hermann, Chair Vascular Neurology and
Dementia at University of Duisburg-Essen, Germany. That project consists in studying the role of
NRG-1 in the brain after ischemia. One doctoral student, Miss Jeismar Carballo, recently finished on
year appointment at Dr. Hermann’s Lab. to finish her doctoral thesis with DAAD funding. Funding:
IDEA Institute-Venezuelan Ministry of Science and Technology, 2008-Present. Role: Coordinator.
3) Project: Technologies to improve the diagnosis of the most frequent cancer. Grant Nº 2008001971.
Objective: Development of Technologies to identify risk factors and improve diagnosis in breast
cancer in Venezuelan population. Funding: Venezuelan Ministry of Science and Technology, 2012-
2015. Role: Coordinator.
4) Project: New kits for diagnosis of metabolic and congenital diseases in the neonatal period. Grant
Nº 2007001066.Objective: Standardize the use of FISH (Fluorescent In Situ Hybridization)
technique for molecular diagnosis of mitochondrial diseases. Funding: Venezuelan Ministry of
Science, 2007-2015. Role: Collaborator.
VII. Invited Talks
1) “Multiples approaches to Study Neuronal Differentiation and other Cellular processes”. Speaker
at the training course: “Introduction to Tissue Culture”. Universidad Simón Bolívar. Caracas,
Venezuela. December 16, 2011.
2) “Advanced Techniques to Perform Imaging Experiments with Live Cells: Neurons, Sometime
Very Difficult to Imagine”. Speaker at the training course: Confocal Microscopy Course. Instituto
de Estudios Avanzados-IDEA. Caracas, Venezuela, May 5-14, 2008.
3) “Molecules, Functions and Mechanisms in the Central Nervous System”. Instituto de Estudios
Avanzados-IDEA, Caracas, Venezuela. April 2007.
4) “Cloning and Expression of Proteins Related to the function in the Central Nervous System”.
Speaker at the International Workshop: “Toxins and Channels: basic science and its applications”.
Instituto de Estudios Avanzados-IDEA. Caracas, Venezuela, July 24-28, 2006.
5) “Function of Neuregulins in the Central Nervous System”. Speaker at the Scientific workshop
from IDEA Research Institute. Instituto de Estudios Avanzados-IDEA, Caracas, Venezuela,
November 2005.
VIII. Participation in Scientific Meetings.
Total of 33 meetings (22 International), here listed from 2010 to 2013
1) Longart M, Bonetti V, Carballo J, Garcia L (2013). Voltage Dependent Sodium Channel
Isoforms are Expressed in Different Subcellular Compartments during In Vitro Development in
Hipppocampal Neurons. American Society for Cell Biology, Annual Meeting. New Orleans, LA.
December, 14-18, 2013, USA.
2) Garcia L, Forsyth P, Castillo C, Longart, M (2012). Factors Released by Sciatic Nerves Affects
Axon Maturation in Hippocampal Neurons. Society for Neuroscience, New Orleans, LA. October
13-17, 2012. USA.
3) García L, Forsyth P, Tirado M, Castillo C, Longart M (2012). Los Receptores ErbB están
Implicados en la Diferenciación Neuronal de Celulas PC12 Mediada por Factores Liberados por
Nervios Ciáticos. (ErbB Receptors are implicated in Neuronal Differentiation of PC12 Cells
Trigered by Sciatic Nerve Factors). I Venezuelan Meeting of Science, Technology and Innovation.
Caracas, September 23-26. Venezuela.
4) Carballo J, Longart M (2012). El Receptor ErbB1 en Ausencia de Ligando se Localiza en el
Núcleo de las Neuronas del Hipocampo de Rata. I Venezuelan Meeting of Science, Technology and
Innovation. Caracas, September 23-26. Venezuela.
5) Forsyth P, Garcia L, Tirado M, Longart M, Castillo C (2012). Inhibición de la via de
señalización de la PKC promueve la expresión de corrientes de Sodio en Células PC12. I
Venezuelan Meeting of Science, Technology and Innovation. Caracas, September 23-26. Venezuela.
6) Carballo J, Sánchez F, Núñez J, Castillo C, Garcia L, Longart M (2012). Efecto del Medio
Condicionado Por Nervio Ciático En La Angiogénesis De Nervios Periféricos Regenerados
Mediante Tubulización (Effect of Sciatic Nerve Conditioned Medium in Angiogenesis in
Peripheral Nerves Regenerated by Tubulization). XV Venezuelan Meeting of MIcroscopy and
Microanalisis (CONVEMI). July 21-27. Santa Ana de Coro, Venezuela.
7) Garcia L, Castillo C, Longart M (2012). Factores liberados por nervios ciaticos afectan la
expresión de Neurofilamantos H (Factors Released by Sciatic Nerves Affect the Expression of
Neurofilaments-H). XV Venezuelan Meeting of MIcroscopy and Microanalisis (CONVEMI), July
21-27. Santa Ana de Coro, Venezuela.
8) Garcia L, Castillo C, Longart M (2011). Factores Liberados por Nervios Periféricos Aumentan la
Proporción de Neuronas Gabaérgicas en Cultivos de Células del Hipocampo (Factors Released by
Peripheral Nerves Increase the Proportion of Gabaergic Neurons in Hippocampal Cell Cultures).
LXI Venezuelan Annual Convention for Advance of Science (Convención Anual de la Asociación
Venezolana para el Avance de la Ciencia, AsoVaC). November 13-18. Maracay, Venezuela.
9) Carballo J, Sánchez F, Núñez J, Castillo C, Longart M (2011). Efecto del Medio Condicionado
Proveniente del Nervio Ciático de Rata sobre la Regeneración y Re-mielinización de axones
periféricos (Effect of Sciatic Nerve Conditioned Medium on Regeneration and re-Mielinization of
Peripheral Axons). 11th Interamerican Congress in Microscopy CIASEM 2011. September 25-29
Septiembre. Yucatán, Mexico.
10) Correa G, Florez I, Longart M, Ramirez M, Cantillo J, De Lucca M (2010). FISH Aplicado a la
Detección de Depleciones del ADN Mitocondrial (Use of FISH to Detect Depletions of
Mitochondrial DNA). LX Venezuelan Annual Convention for Advance of Science (Convención
Anual de la Asociación Venezolana para el Avance de la Ciencia, AsoVaC). November 14-19.
Ciudad Bolívar, Venezuela.
11) Trigos AS, García L, Núnez J, Longart M, Medina R (2010). Efecto de un Medio Condicionado
de Nervios Ciáticos de Ratas Carente de Pro-NGF en la Polarización de Células PC12 (Effect of Rat
Sciatic Nerve Conditioned Médium Depleted of Pro-NGF in Polarity of PC12 Cells). LX
Convención Anual de la Asociación Venezolana para el Avance de la Ciencia (AsoVaC).
November 14-19. Ciudad Bolívar, Venezuela.
12) Garcia L, Nuñez J, Carballo J, Correa G, Castillo C, Longart M (2010). Sciatic Nerve
Conditioned Medium containing BMP7, proBDNF and MAG, affects Dendrite Complexity, Axons
and favors Glial Development. Fellow Poster Session, Society for Neuroscience. November 13-17.
San Diego, USA.
IX. Teaching Experience and Mentorship
Mentor of Graduate Students
1) Lic. Jeismar Carballo, Doctoral Student (2010-Present). Miss Carballo performed one year
appointment (September 2014-September 2015) with DAAD funding, to finish her doctoral thesis
under the supervision of Dr. Dirk Hermann. Chair Vascular Neurology and Dementia at University
of Duisburg-Essen, Germany. Thesis Title: Role of Neuregulin-1-β1 and factors released by sciatic
nerves on the plasticity of the nervous system after injury.
2) Lic. Lisbeth García. Degree: Doctor in Biological Sciences. Universidad Simón Bolívar. Caracas,
Venezuela. May 13, 2013. Thesis Title: Effect of factors released by peripheral nerve in the cell
differentiation of PC12 cells and hippocampal cells.
Mentor of Undergraduate Students
1) Ms. Valeria Bonetti. Degree: Licenciate in Biology. Universidad Simón Bolívar. Caracas,
Venezuela. November 11, 2010. Thesis Title: Location of Voltage-Dependent Sodium Channels in
Rat Hippocampal Neurons.
2) Ms. Giselle Correa. Degree: Licenciate in Biology. Universidad Simón Bolívar. Caracas,
Venezuela. February 2, 2008. Thesis Title: Establishment and Characterization of Hippocampal
Neuronal Primary Cultures.
Member of the Committee of Graduate Students: Jury of Doctoral Thesis
1) Is the Environment or the Genetics? Role of GABA and Dopamine Systems in Mental Disorders:
Study Cases: Depression and Schizophrenia. Presented by: MSc. Miguel Skirzewski Prieto.
Universidad de los Andes. Junio 2013. Mérida, Venezuela.
2) Study of Calmodulin by different Tyrosine Kinases and its effect in some Target Enzymes.
Presented by: BSc. María Valentina Salas Cuevas. Universidad Central de Venezuela. December
2007. Caracas, Venezuela.
Member of the Committee of Undergraduate Students: Jury of Bachelor Thesis
1) Cloning and Expression of Sensory and Motor Neuron-Derived Factor. Presented by Miss
Sayleska Solorzano. Universidad Simón Bolívar. September 2015, Caracas, Venezuela.
2) Effect of a Sciatic Nerve Conditioned Medium Lacking of Pro-NGF on the PC12 Cell
Polarization. Presented by: Miss Anna Sofía Trigos. Universidad Simón Bolívar. November, 2010.
Caracas, Venezuela.
3) Establishment and Characterization of Olygodendrocyte Cultures from Optical Nerves. Presented
by Mr. Simón F. Marques. Universidad Simón Bolívar. September 2008. Caracas, Venezuela.
4) Determination of the osteogenic potential of hydroxyapatite on human mesenchymal stem cells
by evaluating the expression of osteoblast-associated genes. Presented by Mr. Gustavo Rico.
Universidad Simón Bolívar. April 2008. Caracas, Venezuela.
5) Study of the effect of a conditioned medium by optic nerves on PC12 cells. Presented by Mr.
Arnaldo Parra. Universidad Simón Bolívar. February 2008. Caracas, Venezuela.
6) Establishment of Oligodendrocytes Cultures from Optic Nerve of Sprague-Dawley adult rats.
Presented by Miss Lisbeth García, Universidad Simón Bolívar, May 2006. Caracas, Venezuela.
7) Cloning of the truncated isoforms of the neuregulin-1 -3 gene (nrg1-3) of Rattus norvegicus.
Presented by Mr. Gian Carlo Leocata. Universidad Simón Bolívar. May 2006. Caracas, Venezuela.
Advisor of internships and Seminars (graduate and undergraduate): Total of 25
X. Departmental Service: Organization of Scientific Events and Administrative Duties.
1) Organizer of the course: First International Course of Tumor Tissue Biobanks (Primer Curso
Internacional de Biobancos de Tejidos Tumorales). Universidad Central de Venezuela (and other
Institutions). Caracas, Venezuela. October 28-30, 2013 (24 hours).
2) Coordinator and organizer of the Workshop: Strengthening Project in Strategic Networks and
New Modes of Scientific Production and Technology Transfer. Fundacion Instituto de Estudios
Avanzados-IDEA. Caracas, 20 and 21 June 2013. Venezuela. Grant FONACIT No PC: 201302199.
4) Organizer of the: “Confocal Microscopy Course, workshop for users of Carl Zeiss systems”.
Instituto de Estudios Avanzados-IDEA, Caracas, Venezuela. May 5-14, 2008.
5) Organizer of Keynote Lectures by Dr. John Murray. Full Professor at the University of
Pennsylvania (Philadelphia, United States). Instituto de Estudios Avanzados-IDEA, Caracas,
Venezuela. May 21-26, 2006.
XI. Languages: Spanish (mother tongue), English (fluent) and German (Basic, Level A1).
XII. Review Assignments
Scientific Journals and project proposals
1) Referee of research articles. Journal: Frontier in Cellular Neuroscience. Date: April-October
2015.
2) Referee of grant proposal for training courses. Institution: United Nations University-Programme
for Biotechnology in Latin America and the Caribbean. Date: September-October 2015
3) Referee of research articles. Journal: Cellular and Molecular Biology Letters. Date: February-
June 2013.
4) Referee of research articles. Journal: International Journal of Biochemistry. Date: February 2011.
References for Dr. Marines Longart
1) Dr. Andres Buonanno
Chief, Section on Molecular Neurobiology
Deputy, NICHD, Program on Developmental Neurobiology
Building 35, Room 2C-1000
35 Lincoln Drive
National Institutes of Health
Bethesda, MD 20892-3713
Ph (Off): + 1 (301) 496 0170
Ph (Lab): + 1 (301) 496-3298
Fax: + 1 (301) 480-2707
E-mail: buonanno@mail.nih.gov
2) Prof. Dirk M. Hermann
Chair of Vascular Neurology and Dementia.
Klinik für Neurologie Universitätsklinikum Essen.
Hufelandstr. 55, 45122 Essen.
Ph (Off): +49 (201) 723-2180
FAX: +49 (201) 723-5534.
E-Mail: dirk.hermann@uk-essen.de
3) Dr. Detlef Vullhorst
Senior Research Fellow. Section on Molecular Neurobiology
Building 35, Room 2C-1002.
35 Lincoln Drive
National Institutes of Health (NIH).
Bethesda, MD20892, USA.
Ph (Off): + 1 (301) 451 0942
Ph (Lab): + 1 (301) 496-3298
FAX: + 1 (301) 480 2707
E-mail: vullhord@mail.nih.gov
4) Prof. Jose Bubis
Full Professor
Department of Cell Biology, Simon Bolivar University
Caracas, Venezuela
Phone: +58-212-9064219
E-mail: jbubis@usb.ve

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longart_m_CV- May 2016

  • 1. CURRICULUM VITAE I Personal Name: Marines Victoria Longart Marcano Citizenship: Venezuelan Current Position Principal Investigator: August 2005-Present Neuroscience Unit, Instituto de Estudios Avanzados-IDEA. Carretera Nacional Hoyo de la Puerta, Valle de Sartenejas-Baruta. Caracas, Edo. Miranda, 1080. Venezuela. Phone: 58-212-9035191. II. Education 1994-1999: Graduate Studies in Biological Sciences, Department of Cell Biology, Universidad Simón Bolívar (USB). Caracas, Venezuela. Doctor in Biological Sciences (Ph.D), 1999 1987- 1994: Undergraduate studies. School of Sciences: Department of Biology, Universidad de Oriente (UDO). Cumaná, Venezuela. Licentiate in Biology (BS), 1994 Other Courses 1) 1st Meeting on Rare Diseases. Fundación Instituto de Estudios Avanzados (IDEA). Caracas, March, 2012. Venezuela. 2) Study of Proteins and Antibodies for Biotechnology and Biomedicine. Instituto de Estudios Avanzados-IDEA. Caracas, Venezuela. November 23-27, 2009. 3) Confocal Microscopy Course, workshop for users of Carl Zeiss systems. Instituto de Estudios Avanzados-IDEA. Caracas, Venezuela. May 5-14, 2008. 4) Proteomic Approaches in Molecular Biology: Theory and Practice. Universidad de Buenos Aires. Argentina (co-organized by ICGEB). March 3-14, 2008. 5) Isolation, Establishment and Maintenance of Neuronal Stem Cells of the Postnatal adult Brain. Center for Neuroscience Research. Children´s Nacional Medical Center. Washington, December 6-7 United States. 6) Immunocytochemistry, In Situ Hybridization and Live Cell Imaging. Cold Spring Harbor Laboratory. New York, USA. October 18-31, 2003. III. Research and Professional Experience 1) Principal Investigator, Instituto de Estudios Avanzados-IDEA. August 2005-present.
  • 2. 2) Visiting Fellow, National Institutes of Health (NIH), Bethesda, MD, USA. August 2000- July 2005 (Supervisor: Dr. Andres Buonanno). 3) Postdoctoral Researcher, Instituto de Estudios Avanzados- IDEA, Caracas, Venezuela 1999- 2000. 4) Doctoral Student, Instituto de Estudios Avanzados-IDEA, Caracas, Venezuela 1995-1999. 5) Research Assistant. Genetics of Yeast, Centro Tecnológico Polar. Caracas, Venezuela, 1995. 6) Teaching and Research Assistant. Photosynthetic Metabolism. Universidad Simon Bolivar (USB), Caracas, Venezuela, 1994-1995. IV. Scientific Publications Articles in Scientific Journals 1) Bonetti V, Carballo J, Garcia L, Longart M (2016). Nav1.7 Sodium Channel Isoform is Expressed in Growth Cones of Hippocampal Neurons in Culture. Under review at Epilepsy Journal (Corresponding author). 2) Sanchez-Mendoza E, Carballo J, Longart M, Hermann D, Doeppner T (2016). Implantation of Miniosmotic Pumps and Delivery of Tract Tracers to Study Brain Reorganization in Pathophysiological Conditions. J Vis Exp. Jan 18; (107). doi: 10.3791/52932. 3) Trigos A, Longart M, García L, Castillo C, Forsyth P, Medina R (2015). ProNGF derived from rat sciatic nerves downregulates neurite elongation and axon specification in PC12 cells. Front. Cell. Neurosci. 9:364. doi: 10.3389/fncel.2015.00364. 4) García L, Castillo C, Carballo J, Rodríguez Y, Forsyth P, Medina R, Martínez JC, Longart M (2013). ErbB Receptors and PKC Regulate PC12 Neuronal-like Differentiation and Sodium Current Elicitation. Neuroscience. 236: 88-98. (Corresponding author). 5) Carballo J, Sánchez F, Núñez J, Castillo C, García L, Longart M (2012). Efecto del Medio Condicionado por Nervio Ciático en la Angiogénesis de Axones Periféricos Regenerados Mediante Tubulización. (Effect of Sciateic Nerve Conditioned Medium in Angiogenesis of Peripheral Axons Regenerated by Tubulization). Acta Microscópica. Vol 21, supp 2: 87-88. 6) Garcia L, Castillo C, Longart M (2012). Factores Liberados por Nervios Ciáticos Afectan la Expresión de Neurofilamantos H (Factors released by Sciatic Nerves Affect the Expresión of Neurofilaments H). Acta Microscópica. Vol 21, supp B: 89-90. 7) Carballo J, Sánchez F, Núñez J, Castillo C, Longart M (2011). Efecto del Medio Condicionado Proveniente del Nervio Ciático de Rata sobre la Regeneración y Re-mielinización de Axones Periféricos. (Effect of Rat Sciatic Nerve Conditioned Medium on Regeneration and Remielinization of Peripheral Axons). Acta Microscópica. Vol 20, supp B.
  • 3. 8) Correa G, Longart M (2010). Morphometric Analysis of Differentiation Process of Hippocampal Neurons (Análisis Morfométrico del Proceso de Diferenciación in vitro de Neuronas del Hipocampo). Investigación Clínica. 51 (4): 501-518. (Corresponding author). 9) Franco A Y, Longart M (2009). Apliccations of Green Fluorescent Protein (GFP) in Cell Biology and Visualization of the Nervous System (Aplicaciones de la Proteína Verde Fluorescente (GFP) en la Biología Celular y en la Visualización del Sistema Nervioso). Revista de Estudios Transdisciplinarios RET. Fundación Instituto de Estudios Avanzados IDEA. 1 (2): 83-94 (Jul-Dec 2009). (Corresponding author). 10) Longart M, García L, Castillo C, Martínez JC, Medina R, Forsyth P, Malavé C (2009). Sciatic Nerve Conditioned Medium Depleted of Pro-NGF Modulates Sodium Currents and Neurite Outgrowth in PC12 Cells. Neuroscience, 159: 550-558. (Corresponding author). 11) Longart M, Chatani-Hinze M, Gonzalez C, Vullhorst D, Buonanno A. (2007). Regulation of ErbB-4 endocytosis by Neuregulin in cultured GABAergic hippocampal interneurons. Brain Res Bull. 73(4-6): 210-219. 12) Buonanno A, Kwon O-B, Vullhorst D, Hoffman D, Longart M. (2005). Regulation of synaptic plasticity by the NRG-1/ErbB signaling pathway: Possible implications for schizophrenia. Neuropsychopharmacology. 30: S70-S70 Suppl. 13) Kwon O-B*, Longart M*, Vullhorst D, Hoffman D, Buonanno A. (2005). Neuregulin reverses long-term potentiation at CA1 hippocampal synapses. J. of Neuroscience, 25 (41): 9378-9383 (*equal contribution). 14) Longart M, Liu Y, Karavanova I, Buonanno A (2004). Neuregulin-2 is developmentally regulated and targeted to dendrites of central neurons. J Comp Neurol. 472: 156-172. 15) Longart M, Buonanno A (2002). Neuregulins: A family of factors with critical functions during nervous system development and in the cellular transformation and differentiation (Las Neuregulinas: una familia de factores con funciones críticas en el desarrollo del sistema nervioso, y en la diferenciación y transformación celular) Revista de Neurología, 34:91-97. 16) Villegas, R, Villegas G M, Longart M, Hernández M, Maqueira B, Buonnano A, García R, Castillo C (2000). Neuregulin found in cultured-sciatic nerve conditioned medium causes neuronal differentiation of PC12. Brain Res. 852 (2): 305-318. 17) Castrillo M, Aso P, Longart M, Vermehren A (1997). In situ inmunofluorescent of Ribulose-1,5- bisphosphate carboxilase/ oxigenase in mesophyl of C-4 dicotyledonous plants. Photosynthetica 33 (1): 39-50. Chapters in Book 1) Andrés Buonanno, Oh-Bin Kwon, Leqin Yan, Carmen Gonzalez, Marines Longart, Dax Hoffman and Detlef Vullhorst (2008). Neuregulins and neuronal plasticity: possible relevance in schizophrenia. Wiley, Novartis Foundation. Symposium 289: Growth Factors and Psychiatric Disorders. Pages: 165-179. ISBN: 978-0-470-51604-1. Chapter in Book
  • 4. Articles in preparation 1) García L, Castillo C, Correa G, Hernandez M, Longart M (2016). Proteins from Sciatic Nerve Conditioned Medium Influence Glia and Neuronal Hippocampal Cell Fates. (Corresponding author). 2) García L, Castillo C, Hernandez M, Longart M (2016). Sciatic Nerve Conditioned Medium inhibits sodium current induction and Neurofilament-H expression in GABAergic hippocampal neurons (Corresponding author). V. Awards, honors and fellowships 1) Fellowship from the DAAD in the Program Research Stays for University Academics and Scientists (for assistant teachers, assistant professors and lecturers) to work at the Unit of Vascular Neurology, University Hospital Essen. May-August 2016. 2) Member of the Program for Research Support, Level B. National Observatory of Science and Technology (ONCTI). Venezuelan Ministry of Science and Technology (2011-2013; 2013-present). 3) Grant to perform the Workshop: Strengthening Project in Strategic Networks and New Modes of Scientific Production and Technology Transfer. Fundacion Instituto de Estudios Avanzados-IDEA. Caracas, 20 and 21 June 2013. Venezuela. Grant FONACIT No PC: 201302199. Venezuelan Ministry of Science and Technology. 4) Member of the Editorial Board of the Journal: Chinese Journal of Clinicians, 2011-2014. 5) Fellowship to attend the Course "Proteomic Approaches in Molecular Biology" held in Argentina, 03-14 March 2008. FONACIT No. S3-2008000130. Venezuelan Ministry of Science and Technology. 6) Member of research promotion program (PPI), (1997-2002; 2005-2010). National Observatory of Science and Technology (ONCTI), Venezuelan Ministry of Science and Technology. 7) Postdoctoral Fellowship from the National Institutes of Health, August 2000- July 2005. 8) Selection of the publication: “Neuregulin-2 is developmentally regulated and targeted to dendrites of central neurons”. J Comp Neurol. 472: 156-172 (2004), for the journal commentaries Title: Multiple Personalities of Neuregulin Gene Family. Journal of Comparative Neurology 472: 134-139 (2004) by David A. Talmage and Lorna W. Role. 9) Fellowship to perform Graduate Studies, National Research Council (CONICIT) Venezuela, 1995-1999. VI. Research Grants 1) Fellowship from the DAAD within the Program Research Stays for University Academics and Scientists (for assistant teachers, assistant professors and lecturers) to work at the Unit of Vascular Neurology, University Hospital Essen. Project: Modulating Neuregulin-1 to stimulate neurite plasticity after in vitro neuronal injury. Objective: In vitro studies of molecules involved in the
  • 5. morphology and functioning of neurons to identify potential therapeutic agents for neuronal differentiation and regeneration. Funding: Monthly stipend, travel allowance and health insurance are sponsored by DAAD. Consumables, equipments and reagents provided by he host laboratory. Role: Investigator Dates: May-August 2016 2) Project: Role of neurotropich factors contained in a sciatic nerve conditioned medium in neuronal differentiation. Objective: Study of molecules involved in the morphology and functioning of neurons to identify potential therapeutic agents for neuronal differentiation and regeneration. Note: Within this project I have a current collaboration with Dr. Dirk Hermann, Chair Vascular Neurology and Dementia at University of Duisburg-Essen, Germany. That project consists in studying the role of NRG-1 in the brain after ischemia. One doctoral student, Miss Jeismar Carballo, recently finished on year appointment at Dr. Hermann’s Lab. to finish her doctoral thesis with DAAD funding. Funding: IDEA Institute-Venezuelan Ministry of Science and Technology, 2008-Present. Role: Coordinator. 3) Project: Technologies to improve the diagnosis of the most frequent cancer. Grant Nº 2008001971. Objective: Development of Technologies to identify risk factors and improve diagnosis in breast cancer in Venezuelan population. Funding: Venezuelan Ministry of Science and Technology, 2012- 2015. Role: Coordinator. 4) Project: New kits for diagnosis of metabolic and congenital diseases in the neonatal period. Grant Nº 2007001066.Objective: Standardize the use of FISH (Fluorescent In Situ Hybridization) technique for molecular diagnosis of mitochondrial diseases. Funding: Venezuelan Ministry of Science, 2007-2015. Role: Collaborator. VII. Invited Talks 1) “Multiples approaches to Study Neuronal Differentiation and other Cellular processes”. Speaker at the training course: “Introduction to Tissue Culture”. Universidad Simón Bolívar. Caracas, Venezuela. December 16, 2011. 2) “Advanced Techniques to Perform Imaging Experiments with Live Cells: Neurons, Sometime Very Difficult to Imagine”. Speaker at the training course: Confocal Microscopy Course. Instituto de Estudios Avanzados-IDEA. Caracas, Venezuela, May 5-14, 2008. 3) “Molecules, Functions and Mechanisms in the Central Nervous System”. Instituto de Estudios Avanzados-IDEA, Caracas, Venezuela. April 2007. 4) “Cloning and Expression of Proteins Related to the function in the Central Nervous System”. Speaker at the International Workshop: “Toxins and Channels: basic science and its applications”. Instituto de Estudios Avanzados-IDEA. Caracas, Venezuela, July 24-28, 2006. 5) “Function of Neuregulins in the Central Nervous System”. Speaker at the Scientific workshop from IDEA Research Institute. Instituto de Estudios Avanzados-IDEA, Caracas, Venezuela, November 2005. VIII. Participation in Scientific Meetings. Total of 33 meetings (22 International), here listed from 2010 to 2013
  • 6. 1) Longart M, Bonetti V, Carballo J, Garcia L (2013). Voltage Dependent Sodium Channel Isoforms are Expressed in Different Subcellular Compartments during In Vitro Development in Hipppocampal Neurons. American Society for Cell Biology, Annual Meeting. New Orleans, LA. December, 14-18, 2013, USA. 2) Garcia L, Forsyth P, Castillo C, Longart, M (2012). Factors Released by Sciatic Nerves Affects Axon Maturation in Hippocampal Neurons. Society for Neuroscience, New Orleans, LA. October 13-17, 2012. USA. 3) García L, Forsyth P, Tirado M, Castillo C, Longart M (2012). Los Receptores ErbB están Implicados en la Diferenciación Neuronal de Celulas PC12 Mediada por Factores Liberados por Nervios Ciáticos. (ErbB Receptors are implicated in Neuronal Differentiation of PC12 Cells Trigered by Sciatic Nerve Factors). I Venezuelan Meeting of Science, Technology and Innovation. Caracas, September 23-26. Venezuela. 4) Carballo J, Longart M (2012). El Receptor ErbB1 en Ausencia de Ligando se Localiza en el Núcleo de las Neuronas del Hipocampo de Rata. I Venezuelan Meeting of Science, Technology and Innovation. Caracas, September 23-26. Venezuela. 5) Forsyth P, Garcia L, Tirado M, Longart M, Castillo C (2012). Inhibición de la via de señalización de la PKC promueve la expresión de corrientes de Sodio en Células PC12. I Venezuelan Meeting of Science, Technology and Innovation. Caracas, September 23-26. Venezuela. 6) Carballo J, Sánchez F, Núñez J, Castillo C, Garcia L, Longart M (2012). Efecto del Medio Condicionado Por Nervio Ciático En La Angiogénesis De Nervios Periféricos Regenerados Mediante Tubulización (Effect of Sciatic Nerve Conditioned Medium in Angiogenesis in Peripheral Nerves Regenerated by Tubulization). XV Venezuelan Meeting of MIcroscopy and Microanalisis (CONVEMI). July 21-27. Santa Ana de Coro, Venezuela. 7) Garcia L, Castillo C, Longart M (2012). Factores liberados por nervios ciaticos afectan la expresión de Neurofilamantos H (Factors Released by Sciatic Nerves Affect the Expression of Neurofilaments-H). XV Venezuelan Meeting of MIcroscopy and Microanalisis (CONVEMI), July 21-27. Santa Ana de Coro, Venezuela. 8) Garcia L, Castillo C, Longart M (2011). Factores Liberados por Nervios Periféricos Aumentan la Proporción de Neuronas Gabaérgicas en Cultivos de Células del Hipocampo (Factors Released by Peripheral Nerves Increase the Proportion of Gabaergic Neurons in Hippocampal Cell Cultures). LXI Venezuelan Annual Convention for Advance of Science (Convención Anual de la Asociación Venezolana para el Avance de la Ciencia, AsoVaC). November 13-18. Maracay, Venezuela. 9) Carballo J, Sánchez F, Núñez J, Castillo C, Longart M (2011). Efecto del Medio Condicionado Proveniente del Nervio Ciático de Rata sobre la Regeneración y Re-mielinización de axones periféricos (Effect of Sciatic Nerve Conditioned Medium on Regeneration and re-Mielinization of Peripheral Axons). 11th Interamerican Congress in Microscopy CIASEM 2011. September 25-29 Septiembre. Yucatán, Mexico. 10) Correa G, Florez I, Longart M, Ramirez M, Cantillo J, De Lucca M (2010). FISH Aplicado a la Detección de Depleciones del ADN Mitocondrial (Use of FISH to Detect Depletions of
  • 7. Mitochondrial DNA). LX Venezuelan Annual Convention for Advance of Science (Convención Anual de la Asociación Venezolana para el Avance de la Ciencia, AsoVaC). November 14-19. Ciudad Bolívar, Venezuela. 11) Trigos AS, García L, Núnez J, Longart M, Medina R (2010). Efecto de un Medio Condicionado de Nervios Ciáticos de Ratas Carente de Pro-NGF en la Polarización de Células PC12 (Effect of Rat Sciatic Nerve Conditioned Médium Depleted of Pro-NGF in Polarity of PC12 Cells). LX Convención Anual de la Asociación Venezolana para el Avance de la Ciencia (AsoVaC). November 14-19. Ciudad Bolívar, Venezuela. 12) Garcia L, Nuñez J, Carballo J, Correa G, Castillo C, Longart M (2010). Sciatic Nerve Conditioned Medium containing BMP7, proBDNF and MAG, affects Dendrite Complexity, Axons and favors Glial Development. Fellow Poster Session, Society for Neuroscience. November 13-17. San Diego, USA. IX. Teaching Experience and Mentorship Mentor of Graduate Students 1) Lic. Jeismar Carballo, Doctoral Student (2010-Present). Miss Carballo performed one year appointment (September 2014-September 2015) with DAAD funding, to finish her doctoral thesis under the supervision of Dr. Dirk Hermann. Chair Vascular Neurology and Dementia at University of Duisburg-Essen, Germany. Thesis Title: Role of Neuregulin-1-β1 and factors released by sciatic nerves on the plasticity of the nervous system after injury. 2) Lic. Lisbeth García. Degree: Doctor in Biological Sciences. Universidad Simón Bolívar. Caracas, Venezuela. May 13, 2013. Thesis Title: Effect of factors released by peripheral nerve in the cell differentiation of PC12 cells and hippocampal cells. Mentor of Undergraduate Students 1) Ms. Valeria Bonetti. Degree: Licenciate in Biology. Universidad Simón Bolívar. Caracas, Venezuela. November 11, 2010. Thesis Title: Location of Voltage-Dependent Sodium Channels in Rat Hippocampal Neurons. 2) Ms. Giselle Correa. Degree: Licenciate in Biology. Universidad Simón Bolívar. Caracas, Venezuela. February 2, 2008. Thesis Title: Establishment and Characterization of Hippocampal Neuronal Primary Cultures. Member of the Committee of Graduate Students: Jury of Doctoral Thesis 1) Is the Environment or the Genetics? Role of GABA and Dopamine Systems in Mental Disorders: Study Cases: Depression and Schizophrenia. Presented by: MSc. Miguel Skirzewski Prieto. Universidad de los Andes. Junio 2013. Mérida, Venezuela. 2) Study of Calmodulin by different Tyrosine Kinases and its effect in some Target Enzymes. Presented by: BSc. María Valentina Salas Cuevas. Universidad Central de Venezuela. December 2007. Caracas, Venezuela. Member of the Committee of Undergraduate Students: Jury of Bachelor Thesis 1) Cloning and Expression of Sensory and Motor Neuron-Derived Factor. Presented by Miss Sayleska Solorzano. Universidad Simón Bolívar. September 2015, Caracas, Venezuela.
  • 8. 2) Effect of a Sciatic Nerve Conditioned Medium Lacking of Pro-NGF on the PC12 Cell Polarization. Presented by: Miss Anna Sofía Trigos. Universidad Simón Bolívar. November, 2010. Caracas, Venezuela. 3) Establishment and Characterization of Olygodendrocyte Cultures from Optical Nerves. Presented by Mr. Simón F. Marques. Universidad Simón Bolívar. September 2008. Caracas, Venezuela. 4) Determination of the osteogenic potential of hydroxyapatite on human mesenchymal stem cells by evaluating the expression of osteoblast-associated genes. Presented by Mr. Gustavo Rico. Universidad Simón Bolívar. April 2008. Caracas, Venezuela. 5) Study of the effect of a conditioned medium by optic nerves on PC12 cells. Presented by Mr. Arnaldo Parra. Universidad Simón Bolívar. February 2008. Caracas, Venezuela. 6) Establishment of Oligodendrocytes Cultures from Optic Nerve of Sprague-Dawley adult rats. Presented by Miss Lisbeth García, Universidad Simón Bolívar, May 2006. Caracas, Venezuela. 7) Cloning of the truncated isoforms of the neuregulin-1 -3 gene (nrg1-3) of Rattus norvegicus. Presented by Mr. Gian Carlo Leocata. Universidad Simón Bolívar. May 2006. Caracas, Venezuela. Advisor of internships and Seminars (graduate and undergraduate): Total of 25 X. Departmental Service: Organization of Scientific Events and Administrative Duties. 1) Organizer of the course: First International Course of Tumor Tissue Biobanks (Primer Curso Internacional de Biobancos de Tejidos Tumorales). Universidad Central de Venezuela (and other Institutions). Caracas, Venezuela. October 28-30, 2013 (24 hours). 2) Coordinator and organizer of the Workshop: Strengthening Project in Strategic Networks and New Modes of Scientific Production and Technology Transfer. Fundacion Instituto de Estudios Avanzados-IDEA. Caracas, 20 and 21 June 2013. Venezuela. Grant FONACIT No PC: 201302199. 4) Organizer of the: “Confocal Microscopy Course, workshop for users of Carl Zeiss systems”. Instituto de Estudios Avanzados-IDEA, Caracas, Venezuela. May 5-14, 2008. 5) Organizer of Keynote Lectures by Dr. John Murray. Full Professor at the University of Pennsylvania (Philadelphia, United States). Instituto de Estudios Avanzados-IDEA, Caracas, Venezuela. May 21-26, 2006. XI. Languages: Spanish (mother tongue), English (fluent) and German (Basic, Level A1). XII. Review Assignments Scientific Journals and project proposals 1) Referee of research articles. Journal: Frontier in Cellular Neuroscience. Date: April-October 2015.
  • 9. 2) Referee of grant proposal for training courses. Institution: United Nations University-Programme for Biotechnology in Latin America and the Caribbean. Date: September-October 2015 3) Referee of research articles. Journal: Cellular and Molecular Biology Letters. Date: February- June 2013. 4) Referee of research articles. Journal: International Journal of Biochemistry. Date: February 2011. References for Dr. Marines Longart 1) Dr. Andres Buonanno Chief, Section on Molecular Neurobiology Deputy, NICHD, Program on Developmental Neurobiology Building 35, Room 2C-1000 35 Lincoln Drive National Institutes of Health Bethesda, MD 20892-3713 Ph (Off): + 1 (301) 496 0170 Ph (Lab): + 1 (301) 496-3298 Fax: + 1 (301) 480-2707 E-mail: buonanno@mail.nih.gov 2) Prof. Dirk M. Hermann Chair of Vascular Neurology and Dementia. Klinik für Neurologie Universitätsklinikum Essen. Hufelandstr. 55, 45122 Essen. Ph (Off): +49 (201) 723-2180 FAX: +49 (201) 723-5534. E-Mail: dirk.hermann@uk-essen.de 3) Dr. Detlef Vullhorst Senior Research Fellow. Section on Molecular Neurobiology Building 35, Room 2C-1002. 35 Lincoln Drive National Institutes of Health (NIH). Bethesda, MD20892, USA. Ph (Off): + 1 (301) 451 0942 Ph (Lab): + 1 (301) 496-3298 FAX: + 1 (301) 480 2707 E-mail: vullhord@mail.nih.gov 4) Prof. Jose Bubis Full Professor Department of Cell Biology, Simon Bolivar University Caracas, Venezuela Phone: +58-212-9064219 E-mail: jbubis@usb.ve