Training managment training 14-18 feb,2021

1. Atoms for Food and Agriculture: Meeting the Challenge
Plant Tissue Culture Techniques for the
use of Mutation Induction in Plant
Breeding
Fatma SARSU
Technical Officer
Joint FAO/IAEA Programme of
Nuclear Techniquesin Food and Agriculture

2. Outline
plant tissue culture: quick review
Mutationinduction: quick review
Application of mutationinduction on in
vitro cultures

3. Plant Tissue Culture?
Leaf explants
Immature
cotyledon 30 days after
initiation
weeks after
after initiation
A technique of growing in vitro all plant parts whether a single cell , a
tissue or an organ on synthetic medium under controlledand aseptic
conditions
Totipotencytheory states that cells are autonomicand in principle are
capable of regenerating to give a completeplant
Plant cell totipotency !
Cotyledon 4

4. History of plant tissue culture
Cellular theory (Cell is autonom and totipotent, the basic
unit of life) Schleiden-Schwann 1838-39
First attempts to cultivate isolated plant cell in vitro on a
nutrient medium –Harberlandt 1902
White (1934) continuouslygrowing callus culture
MS media- (Murashige and Skoog, 1962)
Haploids from pollen grains of Datura –Guha and
Maheswari,1964
Fusion of protoplasts-Power1970
Plants from tobacco protoplasts-Takebe 1971
Somaclonal variation –Larkin and Scowcroft-1981
PTC applications have been widely developed in last few
decades in worlwide

5. Plant Tissue Culture Facilities(1)
A general washing area
A media preparation, sterilization and storage
area (incubators, centrifuges, autoclaves…)
An aseptic transfer area ( air flow cabinet…)