this is all about HCV

1. Hepatitis C
(HCV)
Mohsin Ali
MSc Medical Virology
Jamia Hamdard

2. Hepatitis C
Family
 Hepatitis C virus (HCV) is an enveloped, positive-strand RNA virus classified in the
Hepacivirus genus within the Flaviviridae family.
 The Flaviviridae are a family of positive, single-stranded, enveloped RNA viruses.
They are found in arthropods, (primarily ticks and mosquitoes), and can occasionally
infect humans.
 The Flaviviridae family includes yellow fever virus, West Nile virus, and dengue virus.

3. HCV - Structure
 small, enveloped,positive single-strandedRNA virus
 50-60nm with linear,single- strandedRNA genome, enclosed
carrying glyproteinspike
 Envelop-Theenvelopeproteins playa role in host receptor binding,
endosome-lipidmembrane fusion, and assembly
 Lipid-Thecholesterolcontent of the HCV particle is higher when
compared with host cell membranes give composition to resembles
human very-low-densitylipoprotein(VLDL)
 Capsid-The HCV capsid, also knownas the HCV core, is the protein
shell thatencapsulates and protects the HCV RNA.
 Nucleocaspid-TheHCV nucleocapsid consists of the HCV capsid plus
the HCV RNA
 HCVRNAgenome-approximately9,600 nucleotide bases in length
 LipoviralParticle-Theformation of the lipoviralparticle facilitates
HCV entryinto hepatocytesand it protects HCV from antibody
neutralization.

4. HCV RNA Genome
 HCV RNA (genome) consists of a single-stranded, positive-sense RNA approximately 9,600
nucleotide bases in length.
 The HCV genome contains a single, long, open reading frame (3,006-3037 codons) flanked by 5'
and 3' untranslated regions (UTRs)
 HCV RNA genome is used both for translation and transcription
 The 5' region, which is upstream from the open reading frame, is approximately 340 nucleotides
long
 IRES is a highly structured regulatory element which is essential in the translation of the open
reading frame. The 3' region is approximately 225 nucleotides long
 The 3'X region serves as a key factor in switching between translation and RNA transcription of the
HCV RNA.

5. Mechanisms (how it cause)
1. BINDING
2. ENDOCYTOSIS
3. FUSION&
UNCOATING
4. TRANSLATION
5. PROCESSING
6. RNA REPLICATION
7. ASSEMBLY
8. MATURATION
9. RELEASE