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NIGERIAN SOCIETY FOR BIOCHEMISTRY AND
MOLECULAR BIOLOGY
37 Annual Scientific Conference @ Umaru Musa Yar’adua University, Katsina State Nigeria
2019
Assessment of Nutritional status of Children in Birnin Kebbi orphanage home,
Kebbi State Nigeria
Yusuf, A. B1 and Abubakar, J1
1Department of Biochemistry and Molecular Biology, Federal University, Birnin Kebbi, Kebbi State, Nigeria.
1
• Malnutrition simply refers
to deviation from adequate
or optimal nutritional
status, which may result
from inadequate or excess
of
• one or
• more of the essential
nutrients (Young, 2012).
MALNUTRITION
UNDERNUTRITION
OBESITY
OVERNUTRITION
Micronutrient
deficiency
2
INTRODUCTION CONT’D
• Malnutrition is a serious public health problem affecting so
many children in Nigeria.
• In Nigeria 37% of children under-five were stunted, 27% were
underweight and 7% of the children were wasted (NDHS, 2018).
• Several studies have been conducted on the nutritional status of
children in Kebbi State by different Non-governmental
organization.
• However, the nutritional status of children in orphanages
particularly in Birnin Kebbi metropolis has received little or no
attention
3
• Therefore, this study was initiated to bridge this gap by
assessing the dietary pattern and nutritional status of children
in Birnin Kebbi Orphanage Home
INTRODUCTION CONT’D
4
AIMS AND OBJECTIVES
• The aim of this study is to assess the nutritional status of
children in orphanage in Birnin Kebbi Metropolis.
• The specific objectives were to:
• Evaluate the dietary pattern of the children
• Screen the children for acute malnutrition using MUAC
• Assess effect of dietary intake on anthropometric, biochemical
and haematological parameters
5
MATERIALS AND METHODS
 Chemicals and reagents
 The chemicals and reagents used in this study were of
analytical grade.
 All from Randox Laboratories Northern Ireland,
 Sigma-Aldrich Chemie GmbH, Germany.
6
Dietary pattern
assessment
24 Hours
&
3day
• Food recall as
described by
Schroder et
al.,(2001).
7
& Physical Examination
Anthropometric
measurements
•Height
•Weight
•MUAC
•Skinfolds thickness &
•Sex of the child was also indicated
According to acceptable standard and in
agreement with WHO recommendations
(WHO, 2006).
8
Anthropometric Measurements and Physical
Examination Cont’d
• Physical examination for signs of Protein Energy Malnutrition
was done as described by Sandstead and Anderson (1947) and
Jelliffe (1966).
9
• Serum total protein was determined according to the
method of Lowry et al. (1951)
• Serum albumin using Bromocresol Green (BCG) Method as
modified by Doumas et al. (1971)
•Estimation of Hematological Parameters by Full Blood Count
(FBC) according to method of Cheesbrough, (2005)
BIOCHEMICAL ANALYSIS
10
• Serum cholesterol by method of Roeschlau et al.
(1974)
• Serum triglyceride by method of Jacobs and Van-
Dermark (1960)
• Serum HDL by method of Lopez-Virella et al. (1977).
BIOCHEMICAL ANALYSIS
11
Study Area
• The study was conducted at Birnin
Kebbi Orphanage Home located in
the Kebbi State Ministry for Women
Affairs and Social Development
Gesse Phase 1, Birnin Kebbi, Kebbi
State Nigeria.
Study subjects
• 40 children under the age of five in
the orphanage participated in this
study. 27 were males and 13 were
females.
Ethical clearance
12
Data Analysis
• Data were subjected to:
• Descriptive statistics and
• Student’s t-test using Statistical Package for Social
Sciences (SPSS), version 20, 2012 SPSS, Inc,
Chicago, IL, USA
• ENA SMART software was used for
anthropometric parameters. The significance
level was established at p ˂ 0.05.
13
RESULTS
Figure 1.0: The dietary consumption pattern of the participants
0
5
10
15
20
25
30
35
40
Cereals Vitamin A rich
fruits and
vegetables
other fruits
and
vegetables
juices Milk and
dairy product
Animal/flesh
food
Legumes and
nut
39.51
9.96
3.99 4.12
14.64
15.8
11.93
14
Table 1: Nutritional Status of the Participants Based on MUAC Measurements
MUAC (cm) Frequency Percentage (%)
<11.5 3 7.5
11.5-12.5 3 7.5
>12.5
Total
34
40
85
100
MUAC ˂11.5cm indicates severe acute malnutrition, MUAC of 11.5cm-12.5cm indicates
moderate acute malnutrition, MUAC ˃ 12.5 indicates adequate nutrition.
15
Table 2: Percentage of Children Showing Physical Signs of Malnutrition
Body part Examined Percentage (%)
Hair (n=8) 20
Eye (n=12) 30
Mouth and Gum (n=1) 2.5
Finger nails (n=3) 7.5
(n= number of children with sign of nutritional deficiency)
16
<10th
Percentile
10 to 90th
Percentile
>90th
Percentile
Triceps skinfold 9 (22.5) 31(77.5) 0(0)
Subscapular 0(0) 40(100) 0(0)
Supraliac 0(0) 40(100) 0(0)
Table 3: Skin folds results of the children according to Percentile Categories Distributions
˂10th percentile indicates inadequate nutrition, 10th to 90th percentile indicates
adequate nutrition, and ˃90th percentile represent over nutrition.
17
Parameter Mean± SD
Serum total protein (g/dl) 5.72±0.83
Serum albumin (g/dl) 3.34±0.56
Serum total cholesterol (mg/dl) 137.38±23.29
Serum triaglycerides (mg/dl) 54.87±19.34
Serum HDL (mg/dl) 29.06±7.18
Hemoglobin (g/dl) 11.27±1.44
Table 4: Mean result of some Biochemical and hematological parameters of the children
Key: Results are mean ± standard deviation
18
0
10
20
30
40
50
60
70
80
90
100
Figure 2: Results of some biochemical and hematological parameters of the children
19
Figure 3: Prevalence of stunting, underweight, and wasting among the children in the
orphanage
20
Figure 4: Prevalence of stunting, underweight, and wasting by gender among the children
in the orphanage
21
Conclusion
is vital for children to reach their full
developmental potential.
revealed high prevalence of
undernutrition among the children in
the orphanage.
This may be
Therefore,
it is important to initiate and sustain
optimal feeding practices in the
orphanage.
associated poor dietary diversity as
revealed by food consumption pattern.
22
Recommendations
From the findings so far, the following recommendations were made:
• Growth monitoring for early detection of malnutrition.
• Nutrition education
• Optimal feeding practices
• Proper hygiene
• Use of micronutrient powder
• Further study should be conducted to assess micronutrient deficiency among
children in the orphanage.
• Finally, Government, NGOs and other stake holders in the community should
help in providing adequate support to these children
23
Thank you
24
Reference
• Adegbusi, H. S. and Sule, M. S. (2011). Anthropometric and biochemical
assessment among under five children in Kusada Local Government Area, Katsina
State, Nigeria. Bayero Journal of Pure and Applied Sciences, 4(2): 137 – 140
• Alabi, A., Macellina, Y., Ijadunola, O., A., Adedeji, O. and Olufemi, A. (2016).
Assessment of Childhood Nutritional Status: Findings from a Health and
Demographic Surveillance System. International Journal of Clinical Nutrition, 4
(1): 7-11.
• Cheesbrough, M. (2005). District Laboratory Practice in Tropical Countries.
Cambridge University Press: Pp 274-279.
• Clerke, R., D. H., Martha, B., A., David, B., D., S., Glenn, B. and Peter, J. (2006)
Malnourishment in a Population of Young Children with Severe Early
Childhood Caries. Pediatric Dentistry, 28:3
• Doumas, B.T. Watson, W. and Biggs, H.G. (1971). Albumin Standards and the
Measure-ment of Serum Albumin with Bromocresol Green. Clinica Chimica Acta,
3:187-196.
• Elizabeth, M., W. and Makokha, A., N. (2013). Nutrition Status of Children in
Orphanages in Selected Primary Schools within Dagoretti Division Nairobi, Kenya.
Journal of Nutrition and Food Science, 4: 248.Biochemistry, 23:882.
25
• Friedwald, W.T., Levy, R. I. and Fredrickson, D.S. (1972). Determination of LDL-
Cholesterol concentration without use of the preparative ultracentrifuge.
Clinical Chemistry, 18:499-502.
• Haleemath, S., K.S., Bhima, B., Sayed, A. and Shobith, B. (2017). Assessment of
nutritional status in children of an orphanage. Indian Journal of Clinical
Anatomy and Physiology, 4(2):156-159.
• Jacobs, N. J. and VanDermark, P. J. (1960). Procedure for serum triglyceride.
Archive of Biochemistry and Biophysics, 88:250-251.
• Jelliffe, D. B. (1966). The assessment of the nutritional status of the community.
WHO Monograph No. 53, Geneva.
• Lidia, P. Ana, M., Chinceșan, M., Ioana, M., A., Mărginean, O., B. and Maria, D.
(2013). Evaluation of Anthropometric and Biochemical Status in Children with
Nutritional Deficiency. Acta Medica Marisiensis, 59(6):293-297.
• Lopez-Virella, M.F., Stone, P. and Ellis, S. (1977).Cholesterol determination in high
density lipoprotein separated by three different methods. Clinical
26
• Lowry, O. H., Rosenbrough, N. T., Farr, A. L. and Randall, R. J. (1951). Protein Measurements with Folin Phenol
Reagent. Journal of Biology and Chemistry, 193: 265-275.
• Roeschlau, P., Bernt, E.and Gruber, J.W. (1974). Procedure for determination of serum Total-cholesterol. Clinical
Biochemistry, 12: 403.
• Sandstead, H. R. and Anderson, R. K. (1947). Nutrition Studies; Description of physical signs possibly related to
nutritional status. Public Health Reports, 62:1073-85.
• Save The Children, (2012). A LIFE FREE FROM HUNGER: Tackling child malnutrition. Retrieved from: http//
www.savethechildren.org/site/apps/ka/ct/ Accessed 27/5/2018
• Schroder, H., Covas, M. I., Marrugat, J., Vila, J., Pena, A., Alcałntara, M. and Masiał, R. (2001). Use of a three-day
estimated food record, a 72-hour recall and a food-frequency questionnaire for dietary assessment in a
Mediterranean Spanish population. Clinical Nutrition, 20: 429–437
• Torun, B.and Chew, F. (1994). Protein Energy Malnutrition.Williams and Wilkins Phil. U.S.A. 8th ed. Vol 1: Pp 950 –
974.
• Turnwald, G.H. and Barta, O. (1989). Immunological and Plasma Protein Disorders. Small Animal Clinical
Diagnosis by Laboratory Methods. WB Saunders, eds Philadelphia. Pp 264-282.
• UNICEF, (2013) Improving Child Nutrition: The achievable imperative for global progress.Pp.4.
• Wammanda, R.D. and Adeleke, S. I. (2002). Protein Energy Malnutrition. Analysis of admission and
outcome. Annals of African Medicine.1 (2): 79-83.
• WHO, (2004). Inheriting the world. The atlas of children's health and the environment. Geneva, World Health
Organization.
• WHO, (2006). Nutrition for Health and Development a Global Agenda for Combating Malnutrition.World
Health Organization, Geneva.
• Young, E. M. (2012). Food and Development. Abingdon, Oxon: Routlegde. Pp 36-38.
27

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Assessment of nutritional status of children under five

  • 1. NIGERIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY 37 Annual Scientific Conference @ Umaru Musa Yar’adua University, Katsina State Nigeria 2019 Assessment of Nutritional status of Children in Birnin Kebbi orphanage home, Kebbi State Nigeria Yusuf, A. B1 and Abubakar, J1 1Department of Biochemistry and Molecular Biology, Federal University, Birnin Kebbi, Kebbi State, Nigeria. 1
  • 2. • Malnutrition simply refers to deviation from adequate or optimal nutritional status, which may result from inadequate or excess of • one or • more of the essential nutrients (Young, 2012). MALNUTRITION UNDERNUTRITION OBESITY OVERNUTRITION Micronutrient deficiency 2
  • 3. INTRODUCTION CONT’D • Malnutrition is a serious public health problem affecting so many children in Nigeria. • In Nigeria 37% of children under-five were stunted, 27% were underweight and 7% of the children were wasted (NDHS, 2018). • Several studies have been conducted on the nutritional status of children in Kebbi State by different Non-governmental organization. • However, the nutritional status of children in orphanages particularly in Birnin Kebbi metropolis has received little or no attention 3
  • 4. • Therefore, this study was initiated to bridge this gap by assessing the dietary pattern and nutritional status of children in Birnin Kebbi Orphanage Home INTRODUCTION CONT’D 4
  • 5. AIMS AND OBJECTIVES • The aim of this study is to assess the nutritional status of children in orphanage in Birnin Kebbi Metropolis. • The specific objectives were to: • Evaluate the dietary pattern of the children • Screen the children for acute malnutrition using MUAC • Assess effect of dietary intake on anthropometric, biochemical and haematological parameters 5
  • 6. MATERIALS AND METHODS  Chemicals and reagents  The chemicals and reagents used in this study were of analytical grade.  All from Randox Laboratories Northern Ireland,  Sigma-Aldrich Chemie GmbH, Germany. 6
  • 7. Dietary pattern assessment 24 Hours & 3day • Food recall as described by Schroder et al.,(2001). 7
  • 8. & Physical Examination Anthropometric measurements •Height •Weight •MUAC •Skinfolds thickness & •Sex of the child was also indicated According to acceptable standard and in agreement with WHO recommendations (WHO, 2006). 8
  • 9. Anthropometric Measurements and Physical Examination Cont’d • Physical examination for signs of Protein Energy Malnutrition was done as described by Sandstead and Anderson (1947) and Jelliffe (1966). 9
  • 10. • Serum total protein was determined according to the method of Lowry et al. (1951) • Serum albumin using Bromocresol Green (BCG) Method as modified by Doumas et al. (1971) •Estimation of Hematological Parameters by Full Blood Count (FBC) according to method of Cheesbrough, (2005) BIOCHEMICAL ANALYSIS 10
  • 11. • Serum cholesterol by method of Roeschlau et al. (1974) • Serum triglyceride by method of Jacobs and Van- Dermark (1960) • Serum HDL by method of Lopez-Virella et al. (1977). BIOCHEMICAL ANALYSIS 11
  • 12. Study Area • The study was conducted at Birnin Kebbi Orphanage Home located in the Kebbi State Ministry for Women Affairs and Social Development Gesse Phase 1, Birnin Kebbi, Kebbi State Nigeria. Study subjects • 40 children under the age of five in the orphanage participated in this study. 27 were males and 13 were females. Ethical clearance 12
  • 13. Data Analysis • Data were subjected to: • Descriptive statistics and • Student’s t-test using Statistical Package for Social Sciences (SPSS), version 20, 2012 SPSS, Inc, Chicago, IL, USA • ENA SMART software was used for anthropometric parameters. The significance level was established at p ˂ 0.05. 13
  • 14. RESULTS Figure 1.0: The dietary consumption pattern of the participants 0 5 10 15 20 25 30 35 40 Cereals Vitamin A rich fruits and vegetables other fruits and vegetables juices Milk and dairy product Animal/flesh food Legumes and nut 39.51 9.96 3.99 4.12 14.64 15.8 11.93 14
  • 15. Table 1: Nutritional Status of the Participants Based on MUAC Measurements MUAC (cm) Frequency Percentage (%) <11.5 3 7.5 11.5-12.5 3 7.5 >12.5 Total 34 40 85 100 MUAC ˂11.5cm indicates severe acute malnutrition, MUAC of 11.5cm-12.5cm indicates moderate acute malnutrition, MUAC ˃ 12.5 indicates adequate nutrition. 15
  • 16. Table 2: Percentage of Children Showing Physical Signs of Malnutrition Body part Examined Percentage (%) Hair (n=8) 20 Eye (n=12) 30 Mouth and Gum (n=1) 2.5 Finger nails (n=3) 7.5 (n= number of children with sign of nutritional deficiency) 16
  • 17. <10th Percentile 10 to 90th Percentile >90th Percentile Triceps skinfold 9 (22.5) 31(77.5) 0(0) Subscapular 0(0) 40(100) 0(0) Supraliac 0(0) 40(100) 0(0) Table 3: Skin folds results of the children according to Percentile Categories Distributions ˂10th percentile indicates inadequate nutrition, 10th to 90th percentile indicates adequate nutrition, and ˃90th percentile represent over nutrition. 17
  • 18. Parameter Mean± SD Serum total protein (g/dl) 5.72±0.83 Serum albumin (g/dl) 3.34±0.56 Serum total cholesterol (mg/dl) 137.38±23.29 Serum triaglycerides (mg/dl) 54.87±19.34 Serum HDL (mg/dl) 29.06±7.18 Hemoglobin (g/dl) 11.27±1.44 Table 4: Mean result of some Biochemical and hematological parameters of the children Key: Results are mean ± standard deviation 18
  • 19. 0 10 20 30 40 50 60 70 80 90 100 Figure 2: Results of some biochemical and hematological parameters of the children 19
  • 20. Figure 3: Prevalence of stunting, underweight, and wasting among the children in the orphanage 20
  • 21. Figure 4: Prevalence of stunting, underweight, and wasting by gender among the children in the orphanage 21
  • 22. Conclusion is vital for children to reach their full developmental potential. revealed high prevalence of undernutrition among the children in the orphanage. This may be Therefore, it is important to initiate and sustain optimal feeding practices in the orphanage. associated poor dietary diversity as revealed by food consumption pattern. 22
  • 23. Recommendations From the findings so far, the following recommendations were made: • Growth monitoring for early detection of malnutrition. • Nutrition education • Optimal feeding practices • Proper hygiene • Use of micronutrient powder • Further study should be conducted to assess micronutrient deficiency among children in the orphanage. • Finally, Government, NGOs and other stake holders in the community should help in providing adequate support to these children 23
  • 25. Reference • Adegbusi, H. S. and Sule, M. S. (2011). Anthropometric and biochemical assessment among under five children in Kusada Local Government Area, Katsina State, Nigeria. Bayero Journal of Pure and Applied Sciences, 4(2): 137 – 140 • Alabi, A., Macellina, Y., Ijadunola, O., A., Adedeji, O. and Olufemi, A. (2016). Assessment of Childhood Nutritional Status: Findings from a Health and Demographic Surveillance System. International Journal of Clinical Nutrition, 4 (1): 7-11. • Cheesbrough, M. (2005). District Laboratory Practice in Tropical Countries. Cambridge University Press: Pp 274-279. • Clerke, R., D. H., Martha, B., A., David, B., D., S., Glenn, B. and Peter, J. (2006) Malnourishment in a Population of Young Children with Severe Early Childhood Caries. Pediatric Dentistry, 28:3 • Doumas, B.T. Watson, W. and Biggs, H.G. (1971). Albumin Standards and the Measure-ment of Serum Albumin with Bromocresol Green. Clinica Chimica Acta, 3:187-196. • Elizabeth, M., W. and Makokha, A., N. (2013). Nutrition Status of Children in Orphanages in Selected Primary Schools within Dagoretti Division Nairobi, Kenya. Journal of Nutrition and Food Science, 4: 248.Biochemistry, 23:882. 25
  • 26. • Friedwald, W.T., Levy, R. I. and Fredrickson, D.S. (1972). Determination of LDL- Cholesterol concentration without use of the preparative ultracentrifuge. Clinical Chemistry, 18:499-502. • Haleemath, S., K.S., Bhima, B., Sayed, A. and Shobith, B. (2017). Assessment of nutritional status in children of an orphanage. Indian Journal of Clinical Anatomy and Physiology, 4(2):156-159. • Jacobs, N. J. and VanDermark, P. J. (1960). Procedure for serum triglyceride. Archive of Biochemistry and Biophysics, 88:250-251. • Jelliffe, D. B. (1966). The assessment of the nutritional status of the community. WHO Monograph No. 53, Geneva. • Lidia, P. Ana, M., Chinceșan, M., Ioana, M., A., Mărginean, O., B. and Maria, D. (2013). Evaluation of Anthropometric and Biochemical Status in Children with Nutritional Deficiency. Acta Medica Marisiensis, 59(6):293-297. • Lopez-Virella, M.F., Stone, P. and Ellis, S. (1977).Cholesterol determination in high density lipoprotein separated by three different methods. Clinical 26
  • 27. • Lowry, O. H., Rosenbrough, N. T., Farr, A. L. and Randall, R. J. (1951). Protein Measurements with Folin Phenol Reagent. Journal of Biology and Chemistry, 193: 265-275. • Roeschlau, P., Bernt, E.and Gruber, J.W. (1974). Procedure for determination of serum Total-cholesterol. Clinical Biochemistry, 12: 403. • Sandstead, H. R. and Anderson, R. K. (1947). Nutrition Studies; Description of physical signs possibly related to nutritional status. Public Health Reports, 62:1073-85. • Save The Children, (2012). A LIFE FREE FROM HUNGER: Tackling child malnutrition. Retrieved from: http// www.savethechildren.org/site/apps/ka/ct/ Accessed 27/5/2018 • Schroder, H., Covas, M. I., Marrugat, J., Vila, J., Pena, A., Alcałntara, M. and Masiał, R. (2001). Use of a three-day estimated food record, a 72-hour recall and a food-frequency questionnaire for dietary assessment in a Mediterranean Spanish population. Clinical Nutrition, 20: 429–437 • Torun, B.and Chew, F. (1994). Protein Energy Malnutrition.Williams and Wilkins Phil. U.S.A. 8th ed. Vol 1: Pp 950 – 974. • Turnwald, G.H. and Barta, O. (1989). Immunological and Plasma Protein Disorders. Small Animal Clinical Diagnosis by Laboratory Methods. WB Saunders, eds Philadelphia. Pp 264-282. • UNICEF, (2013) Improving Child Nutrition: The achievable imperative for global progress.Pp.4. • Wammanda, R.D. and Adeleke, S. I. (2002). Protein Energy Malnutrition. Analysis of admission and outcome. Annals of African Medicine.1 (2): 79-83. • WHO, (2004). Inheriting the world. The atlas of children's health and the environment. Geneva, World Health Organization. • WHO, (2006). Nutrition for Health and Development a Global Agenda for Combating Malnutrition.World Health Organization, Geneva. • Young, E. M. (2012). Food and Development. Abingdon, Oxon: Routlegde. Pp 36-38. 27