1. The Protestant Legend
Presented by: Thao Cao
Mentor: Dr. Hiro Nonogaki
Seed Biology Laboratory
The Protestant Legend
http://www.ccmb.res.in/staff/imran/Resources.html

2.  Food
 Genetic information
 Dispersal unit
http://ucsdnews.ucsd.edu/graphics/images/pod%5B1%5D.gif
http://commons.wikimedia.org/wiki/File:Pumpkin_seeds.jpg

3. http://www.haverford.edu/biology/Johnson/Modelingt
heOrganism/Modeling_the_Organism.htmhttp://www.worldofstock.com/closeups/PFO3982.php
http://www.gettyimages.com/detail/sb10065002w-001/Digital-Vision
Broccoli
Cauliflower
Brussels
Sprouts
Brassicaceae
Canola

4. Dry
seed
NF-YA9
http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi
NF-YA3Dry
seed
High
Low
Expression

5. Down-regulation of NF-YA gene by
microRNA is essential to maintain normal
developmental program of seeds.

6. MicroRNA
RISC
RISC
RISC

7. Wildtype
Control
Mutant

8.  Conditional expression
 Chemical
 Alcohol
 Heat Shock
Chemical
Induction

9. Gene of Interest
Promoter
5XUAS
Switch
CsMV
Promoter
Chemical Induction
(ligand: methoxyfenozide)
Courtesy of Dr. Roger Beachy, Donald Danforth Plant Science Center
VP16 Activation GAL4 DNA Binding Ecdysone Receptor
Active

10. Promoter
GUS
Promoter
GUS
Uninduced
Induced

11. Wild type AGE:GUS
- + - +
5 days after Imbibition

12. Uninduced Induced

13. -- + +
NF-YA3 mNF-YA3

14. Actin
- +
NF-YA9
Wild type NF-YA9 mNF-YA9
-- + +

16. Howard Hughes Medical Institute
Dr. Kevin Ahern
Dr. Hiro Nonogaki
Louis Stokes Alliance for Minority Participation
Natalya Goloviznina
Theresa Nguyen
Dr. Cristina Martinez-Andujar
Mariko Nonogaki
Satoru Yamagishi