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Lab Skills
 Gen ChemI& II (102, 104) withlab
o Lab Safety
o Glassware andinstrumentationuse
o Titrations
o Potentiometry
o Fluorometry
o pH meter
o Scale
 OrganicChemI & II and lab(343, 344, 345)
o OrganicSynthesis
o Volumetrictechniques
o Solutionprep
o Extraction
o Filtration(vacuum)
o Separation
o Sublimation
o Distillation
o Chromatography(column,GC,TLC)
o Spectroscopyanalysis:x-ray,IR,UV,visible,NMR,massspectroscopy,Raman
o Columnpacking
o Centrifuge
o Reflux
o Recrystallization
o Rotary evaporator
o Meltingpoint/boilingpointdetermination
 Quantitative Analysis(221) withlab
o Gravimetricanalysis
o Titrimetricprecipitation
o Titrimetry:Monoproticandpolyproticacidsandbases,metal-ioncomplexation,
oxidation-reductionreactions
o Coulometricmethods
o Potentiometry
o Molecularspectroscopy
o Gas and liquidchromatography
o Fluorimetric/colorimetricdetermination
 Microbiology(383) withlab
o Bacterial Staining
o Platingmethods
o Graphinggrowthcurves
o Bacterial identification
o Microscope application(oil immersion)
o Mediaand bufferprep
o Selectivemedia
o Conjugation
o Antibioticassay,plaqueassay,Amestest
 BiochemistryLab(603)
o Batch cultures
o DNA extraction
o PlasmidDNA prep
o Restrictionenzymedigests
o PCR (qPCR,RT-PCR)
o Gel electrophoresis
o SDS-PAGE
o Differential Centrifugation
o Microarray Interpretation
o ProteinAssay(Lowry,Bradford,ligandbinding)
o Western/Northern/Southernblotting
o ProteinPurification (chromatography,ion-exchange)
 BiochemistryProteinStructures(601)
o Proteinmodelingsoftware(pymol)
o BLAST
o FRET interpretation
 SeniorResearch(691)
o Organicsynthesisof drugsfollowinglab notes
o Mice injections
o Rotary evaporator
o Reflux
o TLC plate for productyielddetermination
o Columnpreparationforsample separation
o Centrifugation
o Human and mice cell culturesfordrug interactions
o Drug metabolism(DMPK) assayprep
o Scanningmetaboliteprofilesusingmassspectrometry

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Lab Skills

  • 1. Lab Skills  Gen ChemI& II (102, 104) withlab o Lab Safety o Glassware andinstrumentationuse o Titrations o Potentiometry o Fluorometry o pH meter o Scale  OrganicChemI & II and lab(343, 344, 345) o OrganicSynthesis o Volumetrictechniques o Solutionprep o Extraction o Filtration(vacuum) o Separation o Sublimation o Distillation o Chromatography(column,GC,TLC) o Spectroscopyanalysis:x-ray,IR,UV,visible,NMR,massspectroscopy,Raman o Columnpacking o Centrifuge o Reflux o Recrystallization o Rotary evaporator o Meltingpoint/boilingpointdetermination  Quantitative Analysis(221) withlab o Gravimetricanalysis o Titrimetricprecipitation o Titrimetry:Monoproticandpolyproticacidsandbases,metal-ioncomplexation, oxidation-reductionreactions o Coulometricmethods o Potentiometry o Molecularspectroscopy o Gas and liquidchromatography o Fluorimetric/colorimetricdetermination  Microbiology(383) withlab o Bacterial Staining o Platingmethods o Graphinggrowthcurves o Bacterial identification o Microscope application(oil immersion) o Mediaand bufferprep o Selectivemedia
  • 2. o Conjugation o Antibioticassay,plaqueassay,Amestest  BiochemistryLab(603) o Batch cultures o DNA extraction o PlasmidDNA prep o Restrictionenzymedigests o PCR (qPCR,RT-PCR) o Gel electrophoresis o SDS-PAGE o Differential Centrifugation o Microarray Interpretation o ProteinAssay(Lowry,Bradford,ligandbinding) o Western/Northern/Southernblotting o ProteinPurification (chromatography,ion-exchange)  BiochemistryProteinStructures(601) o Proteinmodelingsoftware(pymol) o BLAST o FRET interpretation  SeniorResearch(691) o Organicsynthesisof drugsfollowinglab notes o Mice injections o Rotary evaporator o Reflux o TLC plate for productyielddetermination o Columnpreparationforsample separation o Centrifugation o Human and mice cell culturesfordrug interactions o Drug metabolism(DMPK) assayprep o Scanningmetaboliteprofilesusingmassspectrometry