This presentation describes the detection of allergens in foods using PCR. For PCR, we designed the PCR primer set to detect specified allergens.

1. Food allergies
Detection of allergens using PCR
Tsukuba GeneTecnology Laboraries Inc.
http://www.tsukuba-genetech.com/index_en.html

2. Nuts allergy

3. Food allergies Detection of Allergens using PCR
Macadamia nuts
Dw：Distilled water
A：Armond
Ks：Cashew nuts
Kl10 ：Walnut
P2：Peanuts
M：Macadamia nuts
Ma：100bp Marker
Detection of
Macadamia nuts

4. Food allergies Detection of Allergens using PCR
Cashew nuts
Dw：Distilled water
A：Armond
Ks：Cashew nuts
Kl ：Walnut
P2：Peanuts
M：Macadamia nuts
Ma：100bp Marker
Detection of
Cashew nuts

5. Food allergies Detection of Allergens using PCR
Armond
Dw：Distilled water
A：Armond
Ks：Cashew nuts
Kl10：Walnut
P2：Peanuts
M：Macadamia nuts
Ma：100bp Marker
Detection of
Armond

6. Food allergies Detection of Allergens using PCR
Peanuts
Dw：Distilled water
A：Armond
Ks：Cashew nuts
Kl10 ：Walnut
P2：Peanuts
M：Macadamia nuts
Ma：100bp Marker
Detection of
Peanuts

7. Food allergies Detection of Allergens using PCR
Walnut
Dw：Distilled water
A：Armond
Ks：Cashew nuts
Kl10 ：Walnut
P2：Peanuts
M：Macadamia nuts
Ma：100bp Marker
Detection of
Walnut

8. Grain allergies

9. Wheat
Detection
of wheat
Dw：Distilled water
Da：Soybeans
Ko：Wheat
So：Soba
Pi：Peanuts
Mk：Macadamia nuts
Ma：100bp Marker
Food allergies Detection of Allergens using PCR

10. Detection
of
Soybeans
Soybeans
Food allergies Detection of Allergens using PCR
Dw：Distilled water
Da1：Soybeans
Da2：Soybeans
Ko：Wheat
So：Soba
Ma：100bp Marker

11. Soba
Food allergies Detection of Allergens using PCR
Detection
of Soba
Dw：Distilled water
Da：Soybeans
Ko：Wheat
So：Soba
Pi：Peanuts
Mk：Macadamia nuts
Ma：100bp Marker

12. Sesame
Detection
of
Sesame
Dw: Distilled water
Sk：Salmon
Sb：Mackerel
Go： Sesame
Ko：Wheat
So：Soba
Ma：100bp Marker
Food allergies Detection of Allergens using PCR

13. Vegetable allergies

14. Yam
Detection
of
Yam
Dw：Distilled water
Ya：Yam
Ko：Wheat
So：Soba
Ma：100bp marker
Food allergies Detection of Allergens using PCR

15. Meat allergies

16. Bovine
Detection
of
Bovine origin
Dw：Distilled water
Us：Bovine
Bu：Porcine
Ni：Chicken
Ma：100bp marker
Food allergies Detection of Allergens using PCR

17. Chicken
Detection
of
Chicken origin
Dw：Distilled water
Us：Bovine
Bu：Porcine
Ni：Chicken
Ma：100bp marker
Food allergies Detection of Allergens using PCR

18. Porcine
Detection
of
Porcine origin
Dw：Distilled water
Us：Bovine
Bu：Porcine
Ni：Chicken
Ma：100bp marker
Food allergies Detection of Allergens using PCR

19. Fruit allergies

20. Peach
Detection
of
Peach
Dw：Distilled water
R：Apple
M：Peach
K：Kiwi fruit
B：Banana
G：grapefruit
Ma：100bp markers
Food allergies Detection of Allergens using PCR

21. Kiwi fruit
Detection
of
Kiwi fruit
Dw：Distilled water
R：Apple
M：Peach
K：Kiwi fruit
B：Banana
G：grapefruit
Ma：100bp markers
Food allergies Detection of Allergens using PCR

22. Banana
Detection
of
Banana
Dw：Distilled water
R：Apple
M：Peach
K：Kiwi fruit
B：Banana
G：grapefruit
Ma：100bp markers
Food allergies Detection of Allergens using PCR

23. Citrus
Detection
of
Citrus
Food allergies Detection of Allergens using PCR
Dw：Distilled water
Mi：mandarins
Ora：Sweet orange
Gra：Grapefruit
Ki：Kiwi fruit
Mo：Peach
Ma：100bp markers

24. Detection
of
Sweet orange
Food allergies Detection of Allergens using PCR
Sweet orange
Dw: Distilled water
M: 100bp markers