occurance of E. coli

1. OCCURRENCE AND ANTIBIOGRAM OF ESCHERCHIA COLI AND
KLEBISELLA PNEUMONIAE IN URINE SAMPLES OF
ASYMPTOMATIC NEW AND FINAL YEAR FEMALE STUDENTS OF
AHMADU BELLO UNIVERSITY
B.Sc. PROJECT PROPOSAL
BY
AMADI CHRISTIANAH CHIAMAKA
(U17MC2007)
SUPERVISOR:
Mr. H. G BISHOP
DEPARTMENT OF MICROBIOLOGY,
FACULTY OF LIFE SCIENCES,
AHMADU BELLO UNIVERSITY ZARIA.
APRIL, 2021.

2. INTRODUCTION
• Asymptomatic bacteriuria is a condition in which urine
cultures reveals significant growth of pathogens that is
greater than 105 bacteria /ml, but the patients show no
symptom of urinary tract infection (Mbata, Aleru, et
al.,2016).
• The frequency varies among different populations, depending
on factors such as age, sex, area of residence and underlying
disorders, pregnancy, obstruction of urinary flow and
underlying disease (Mbata, Aleru, et al.,2016).
• The organisms most frequently isolated in asymptomatic
bacteriuia and UTI includes species of Enteribactericeae
especially Escherichia coli, Klebisella pneumoniae,
Staphylococcus aureus, Pseduomonas aeruginosa and Proteus
species (Mbata, Aleru, et al.,2016).

3. STATEMENT OF RESEARCH PROBLEM
• The danger with bacteriuria is that it does not always
present with symptoms but if left untreated may lead to
acute pyelonephritis and cystitis.
• Female students are more prone to urinary tract infection
because of their anatomical site.
• Some female students do not practice good hygiene and
unawareness how to properly clean up after using the
toilet.

4. JUSTIFICATION FOR THE STUDY
• It is important to detect and treat any asymptomatic UTIs
at an early stage.
• Bacteriuria is treated by antibiotics administration and
there is increased development of microbial resistance to
these antibiotics.
• The comparison between new and final academic levels of
the students will help to evaluate their level of naiveness or
assimilation of health education.

5. AIM
• To determine the occurrence and antibiogram of
Escherchia coli and Klebisella pneumoniae in urine
samples of asymptomatic new and final year female
students of Ahmadu Bello University
OBJECTIVES
i. To isolate and characterize E. coli and K. pneumoniae
from the urine samples of apparently healthy new and
final year female students of ABU.
ii. To determine the antibiotic susceptibility pattern of E.coli
and K. pneumoniae isolate from the female students.
iii. To determine some risk and demographic factors
associated with asymptomatic UTIs among the female
students.

6. METHODOLOGY
Study Area: Ahmadu Bello University, Zaria.
Study population: Female students comprising of new and
final year students.
Collection of samples
• A total of 200 samples of urine (10mls each) will be
collected from the females students.
• Structured questionnaires will be administrated to gather
information from demographic and risk factors.

7. METHODOLOGY CON’T
Isolation
• Each 10ml urine sample will be shaken and transferred to a
plastic centrifuge tube and spun at 3000rpm for five minutes.
• The supernatants will be discarded and the sediments will be
transferred into sterile petri dishes using Pasteur pipette to
which molten MacConkey Agar and Chocolate Agar
Incubation
Biochemical tests
• Catalase test
• Oxidase test
• Coagulase test
• Triple sugar ion agar test
• IMViC test
• Urease test

8. METHODOLOGY CON’T
Antibiogram
• After incubation, the test organisms will be sub-cultured
into Muller Hinton Agar using sterile cotton swabs.
• The antibiotic discs will be placed in each of the Muller
Hinton Agar plates using sterile forceps and incubated at
37°C for 24hrs.