Substrate level phosphorylation and it's mechanism || Biochemistry || B Pharmacy || Project || slideshare || biology || chemistry *images use in this ppt is only for educational purpose In this presentation, i tell about substrate level phosphorylation Phosphorylation involves the transfer of phosphate group from one compound to other. ➢ Substrate level phosphorylation is a direct phosphorylation of ADP with a phosphatase group by using the energy obtain from a coupled reaction. ➢ Occurs in cytoplasm ( glycolysis – due to aerobic and anaerobic condition) and in mitochondrial matrix ( krebs cycle – anaerobic condition)

1. Substrate Level Phosphorylation And
Its Mechanism
Name : Darshan Ratan Jadhav
Class : F.Y.B.Pharmacy
Subject : Biochemistry
College : K.K.Wagh College of
Pharmacy, Nashik

2. Content
•Introduction
•Example
•Reaction
•Reference
Content
• Introduction
• Mechanism
• The role of substrate-level
phosphorylation in aerobic
respiration.
• Refference

3. Substrate Level Phosphorylation
➢ Phosphorylation involves the transfer of phosphate
group from one compound to other.
➢ Substrate level phosphorylation is a direct
phosphorylation of ADP with a phosphatase group by
using the energy obtain from a coupled reaction.
➢ Occurs in cytoplasm ( glycolysis – due to aerobic and
anaerobic condition) and in mitochondrial matrix ( krebs
cycle – anaerobic condition)

4. Genral Substrate Level
Phosphorylation Reaction

5. Mechanism of Substrate level
phosphorylation
• High-energy phosphate groups are transferred
directly from phosphorylated substrates to ADP
• ATP is synthesized via substrate-level
phosphorylation in glycolysis and the Krebs cycle

6. Catalysis

7. The role of substrate-level
phosphorylation in aerobic respiration
• Substrate-level Phosphorylation and Aerobic Respiration:
• We know that there is the formation of 32 ATP molecules in
total (since 2 ATP molecules are used in the beginning of
glycolysis the final ATP yield is 30) as a result of the complete
oxidation of one glucose molecule in aerobic respiration.
• Out of all 32 ATP molecules, 26 are produced by way of
oxidative phosphorylation with the involvement of NADH and
FADH2, whereas the remaining 6 are the result of substrate-
level phosphorylation.

8. 1. During glycolysis, the enzyme phosphoglycerate kinase
transfers the high energy phosphoryal group from the
carboxyl group of 1,3-bisphosphoglycerate to ADP,
forming ATP and 3-phosphoglycerate.

9. during glycolysis, the enzyme pyruvate kinase transfers
the high energy phosphoryl group from
phosphoenolpyruvate to ADP, forming ATP and
pyruvate.
2.
➢ In glycolysis during substrate level phosphorylation
4 ATP molecules are produce.

10. During TCA cycle (Citric acid cycle; Kreb’s cycle), the
enzyme succinyl-CoA synthetase breaks the thioester
bond of succinyl-CoA andthe energy released in this
breakage is used to drive the synthesis of a
phosphoanhydride bond in GTP or ATP. Succinate is the
product in the process.
3.
➢ In This Step 2 ATP is produce

11. Refference
• Biochemistry Book By U. Satyanarayana & U.
Chakrapani.
• www.google.com
• Guidance of subject teacher