Research was conducted to assess the efficacy of a phytase and 25-hydroxycholecalciferol on mineral metabolism and performance of broilers fed a corn-soybean diet. This was done to evaluate a feeding strategy aiming to reduce calcium and phosphorus levels in order to improve efficiency and nutrition in broilers. To find out more about DSM visit: http://www.dsm.com/markets/anh/en_US/home.html

1. Hermes RG1, Iglesias BF2, Azcona JO2, Ioli G1, Charriere
MV2, Hernandez JM1
1DSM
Nutritional Products Ltd.
2INTA, Buenos Aires, Argentina
IPSF - Atlanta, 2013
T118 – 29th, January

2. Feed Costs
Environment
Customers
Save Energy, Protein and Minerals
Nutrionists
Excess of Ca & P

3. VITAMIN D3
Intestinal Ca
absorption
SAVE Ca
+
PHYTASE
Release of
phytate P
SAVE P
Whitehead et al. (2004); Angel et al. (2005);
Weber (2009); Green & Persia (2012)

4. Phytic Acid
p
Phytic Acid
p
Phytic Acid
p
Ca+
Ca+ Ca+ Ca+
Ca+
Ca+
Ca+
Ca+
Ca+
Phytase
Phytic Acid
Phytase
Ca+ Ca+
p
Ca+
Phytase
Phytase
Qian et al. (1997); Angel et al. (2005)

5. “To evaluate a feeding strategy
(combination of Vitamin D3 and
Phytase), aiming for the
reduction on Ca & P levels.”

6. Starter Positive Control
• 720 day-old chicks (Cobb
500). 8 replicates of 15
birds/treat
• 3 feeding phases
• Starter (1-14d)
• Grower (15-28d)
• Finisher (29-48d)
• Pelleted
• 0.8 bar/30 sec./77˚C
Ingredients
Corn
SBM
MBM
Soybean oil
Oyster shell
(%)
58.5
33.7
5.36
1.1
0.45
Nutrients
ME (Kcal/kg)
Protein (%)
Vit. D3 (IU/kg)
3240
21
3000

7. TREATMENTS
PC
NC1
NC2
ME
Ca nPP
(Kcal/kg) (%)
3240 0.9
Positive Control
-75 -0.18
Negative Control 1
-75 -0.28
Negative Control 2
NC1+ADD
NC1 + Phy + 25-OH-D3
NC2+ADD
(%)
0.45
-0.15
-0.2
NC2+ Phy + 25-OH-D3
• 1000 FYT of Citrobacter braakii phytase/kg of feed
RONOZYME® HiPhos GT from DSM Nutritional Products
• 69 ppb 25-OH-D3 (2760 IU of vit D3)/ kg of feed)
HyD® from DSM Nutriotional Products

8.  Animal
Performance
◦ BW, FI, FCR at 14, 28 and 48 days of age
 Tibia
Ash at 48d
 Carcass
Yield at 48d
 Economical
analysis

9.  Complete
Randomized Design
 ANOVA
and Duncan’s multiple range
test were used for means separation
using the infoSTAT software (2011)
 Statistical
significance (P < 0.05)

10. A
480
BW(g)
470
460
B
B
450
C
440
D
430
420
PC
NC1
NC2
FI (g)
541B
534B
515C
562A
547AB
FCR (g:g)
1.19B
1.23A
1.22AB
1.19B
1.20B
ABC Different
NC1+ADD NC2+ADD
letters are significantly different (P<0.05)

11. 1700
BW(g)
1650
A
AB
1600
C
1550
B
D
1500
1450
1400
PC
NC1
NC2
FI (g)
2339AB
2323B
2249C
2387A
2346AB
FCR (g:g)
1.45B
1.52A
1.51A
1.46B
1.47B
ABC Different
NC1+ADD NC2+ADD
letters are significantly different (P<0.05)

12. 3700
A
BW(g)
3650
A
A
B
3600
3550
C
3500
3450
3400
FI (g)
PC
NC1
NC2
6269
6255
6199
6261
6281
1.77AB
1.79A
1.73C
1.74BC
FCR (g:g) 1.72C
ABC Different
NC1+ADD NC2+ADD
letters are significantly different (P<0.05)

13. 52
A
51
B
BC
50
C
49
48
PC
NC1
C
NC2
NC1+ADD NC2+ADD
* 20 right tibias per treatment
ABC Different letters are significantly different (P<0.05)

14. 79.0
A
78.5
78.0
AB
AB
AB
77.5
B
77.0
76.5
CW (g)
PC
2827
NC1
NC2
2780
2674
ABC Different
NC1+ADD NC2+ADD
2827
letters are significantly different (P<0.05)
2830

15. 285
282
ROI
3:1
280
$12
275
272
268
270
$4
266
270
$4
265
260
PC
NC1
NC2
NC1
+ADD
NC2
+ADD

16. 
Nutrient reduction
broilers performance.
negatively
affects

The
combination
of
25-OH-D3
and
phytase, with Ca & P reduction, improved
broilers performance up to similar levels of
PC diet; therefore, reducing feed cost up to
4%.

ROI 3:1

17. 
Bernardo Iglesias and Jorge Azcona

Giancarlo Ioli & José Maria Hernandez
Rafael Hermes, PhD
+55 11 96069-4448
rafael.hermes@dsm.com