Poster presented a the XIVth European Poultry Conference, 23-27 June 2014, Norway. Aureli et al.

1. MATERIALS & METHODS
Animals: 8 x 20 male broilers (Ross PM3) / treatment
Diets/Treatments : Positive control (PC) diets adequate in all nutrient
requirements, test diets (TD) deficient in nutrients and energy
equivalent to the matrix values determined for PHY1 standard
recommended dose (RD), were supplemented with additional DCP to
contain 1.80 g and 1.37 g available P per kg starter and grower diets,
respectively, and fed as negative control (NC) diet. Phytase products
were added to TD diet giving 4 test treatments: PHY1 (C. braakii,
Ronozyme® HiPhos (GT)) at 100 mg, PHY2 (E.coli phytase produced by
Schizosaccharomyces pombe) at 50 mg/kg, PHY3 (E. coli-phytase,
expressed in Trichoderma reesei) at 100 mg/kg and PHY4 (P. lycii,
Ronozyme NP (CT)) at 150 mg/kg.
Feeding: pellets, ad libitum; Duration: day 1 to day 35 of bird age;
Parameters: WG, FCR, apparent ileal digestibility of P (AIDP), tibia
ash percentage ; Statistical analysis: ANOVA followed by Newman-
Keuls test (p<0.05)
Comparative efficacy of four phytase products on growth
performance and apparent ileal digestibility of phosphorus
in broiler chickens
R. Aureli1, M. Umar Faruk1 and J. Broz2
1Research Centre for Animal Nutrition and Health, DSM Nutritional Products France, Village-Neuf, France,
2DSM Nutritional Products Ltd, Basel, Switzerland
Research has demonstrated that phytase is the only enzyme that is able to initiate the release of phosphorus (P)
from the phytate molecule, making it available for absorption and utilization (Selle and Ravindran, 2007). The
industrial demand for phytases with greater potency in intestinal phytate hydrolysis and better heat stability
continues to stimulate the search for new enzyme sources. Enzyme preparations with phytases derived from
Aspergillus ficuum, Peniophora lycii and Escherichia coli are available commercially. More recently, a new microbial
6-phytase produced by synthetic genes, mimicking a gene from Citrobacter braakii, was introduced into the market
(Aureli et al., 2011).
OBJECTIVE: To compare the efficacy of phytase products derived from either C. braakii, E. coli or P. lycii in
terms of performance, ileal P digestibility and bone mineralization in broiler chickens.
CONCLUSIONS
Phytase supplementation resulted in similar effects in terms of bone mineralization
and AIDP
PHY1 and PHY3 resulted in better growth performance compared to PHY2 and PHY4
and performed equally to the positive control, irrespective of the measured
parameters
Table 1: Composition and nutrient contents of the experimental diets
RESULTS
a,b Means within a row, not sharing a common superscript, are significantly different (p<0.05)
Figure 1: Effect of phytase on WG (g/bird) and FCR (day 1-36) Figure 2: Effect of phytase on AIDP and tibia ash percentage
STARTER (Day 1-21) GROWER (Day 22-36)
Ingredients (%) PC NC TD PC NC TD
Soybean meal 39.10 38.30 38.30 37.50 36.40 36.40
Maize 53.79 56.17 56.17 54.04 56.74 56.74
Dicalcium phosphate 1.15 0.60 0.34 0.95 0.35 0.10
Calcium carbonate 0.95 0.74 0.85 0.90 0.95 0.95
DL-Methionine 0.20 0.20 0.20 0.10 0.10 0.10
L-Lysine 0.05 0.05 0.05 - - -
Soya oil 3.50 1.70 1.70 5.20 3.40 3.40
Salt 0.20 0.20 0.20 0.15 0.15 0.15
Vitamin- mineral
premix
1.00 1.00 1.00 1.00 1.00 1.00
Coccidiostat 0.06 0.06 0.06 0.06 0.06 0.06
Sand - 0.74 1.13 - 0.75 1.00
Titanium dioxide 0.10 0.10 0.10
Calculated content
Total P (g/kg) 5.72 4.65 4.17 5.23 4.11 3.64
Av. P (g/kg) 2.73 1.80 1.37 2.37 1.37 0.95
Ca (g/kg) 9.20 7.90 6.80 8.50 7.20 6.60
Analyzed content
Total P (g/kg) 6.10 5.10 4.70 5.10 4.70 4.30
Ca (g/kg) 9.10 8.00 7.10 7.80 7.30 6.70
XIVth European Poultry Conference , Stavanger, Norway 23 – 27 June 2014
b
a a a a a
b c b b b a
References:Aureli et al., (2011) Int. J. Poult. Sci 10(2):160-168. Ravindran et al., (1995b) Poult. Sci. 74:1820–1830.