6th International Scientific Conference on Applied Sciences and Engineering 26-27 January, 2016 Bayview Hotel Singapore

1. Abstract of Applied Sciences and Engineering, 2016, Vol.6
DOI: 10.18488/journal.1001/2016.6/1001.6
6th
International Scientific Conference on Applied
Sciences and Engineering
26-27 January, 2016
Bayview Hotel Singapore
Conference Website: www.scihost.org
3
Paper ID: 07/16/ 6
th
ISCASE
Extraction and Purification of L-Asparaginase Produced by
Acinetobacter Baumannii and Their Antibiofilm Activity
against Some Pathogenic Bacteria
Sahira Nsayef Muslim1
--- Israa M.S. AL_Kadmy2
--- Alaa Naseer Mohammed Ali3
---
Nadheema Hammood Hussein4
--- Ahmed Sahi Dwaish5
--- Ban Talib El-Haboby6
--- Sraa
Nsayef Muslim7
--- Suhad abbas abid8
1,2,3,4,5,6,7,8
Department of Biology, College of Science, AL-Mustansiryia University
Abstract
L-asparaginase is an enzyme catalyzing the hydrolysis of L-asparagine and formation
of L-aspartate and ammonia and widely used as anticancer drug in pharmaceutical and
food industry. Eight Acinetobacter baumannii isolates of were isolated from different
blood and sputum samples and screened for higher L-asparaginase production,
Acinetobacter baumannii Sp3 gave higher asparaginase activity of 7.32 U/ml. L-
asparaginase was purified to homogeneity with ammonium sulfate at 45% saturation
followed by DEAE-Cellulose ion exchange chromatography and sephadex G-100 gel
filtration chromatography with a recovery yield of 68% and 22.65 fold of purification. L-
asparaginase had antibiofilm activity against all tested pathogenic bacteria after using
Congo Red agar and Microtitration plates methods. Highly antibiofilm of L-
asparaginase recorded against Klebsiella pneumoniae followed by Pseudomonas
aeruginosa with reduction of biofilm formation ratio to 32 and 41% ,respectively
compared with (100)% of control. Thus L-asparaginase has promising benefit as
antibiofilm agent against biofilm forming pathogenic bacteria.
Keywords: Purification, Acinetobacter Baumannii, Antibiofilm Activity, Pathogenic Bacteria