5 minutes of fame

1. Introducing the EVOQ LC-Triple Quadrupole
The New Game Changing
LC Triple Quad from
Bruker
Bradley Duruttya
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

2. Introducing the EVOQ LC-TQ
• Bruker Triple Quadrupole MS
• VIP Heated-ESI
• Active Exhaust
• IQ Dual Ion Funnel
• PACER Software
• Mass Range: 10-1250 amu EVOQ Elite
• Bruker Analytical LC
• Advance HPLC (8000 psi)
• Advance UHPLC (15000 psi)
• Advance UHPLC with OLE (15000 psi)
EVOQ QubeTM
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

3. One Mission
EVOQ was designed for a singular purpose – to
reliably quantify thousands of real samples in the
fastest ‘sample-to-report’ time possible.
Food Testing
Environmental Testing
Toxicology
Discovery PK
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

4. Keys to Achieving Mission
Sustained High-Sensitivity
• Orifice interface, Active Exhaust, IQ Dual Ion Funnel and VIP-HESI
Increased Capacity through Higher Robustness
• High-sensitivity, Flat-tuning IQ Dual Ion Funnel
Fastest ‘Sample-to-Report’ time
• Pacer software with Exception Based Data Review
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

5. Active Exhaust vs Equine Plasma – NO DIVERT VALVE
USED
• 500 equine plasma samples analyzed 14.0000
with four calibration curves, two 12.0000
y(1st) = 0.0047x - 0.0141
R² = 0.9987
bracketing, two spread between y(4th) = 0.0047x - 0.0413
samples.
10.0000 R² = 0.9989
8.0000
Area Ra o
• No divergence between calibration 6.0000
curves run before and after samples 4.0000 1st Curve
were run.
2.0000 4th Curve
0.0000
0 500 1000 1500 2000 2500 3000
Concentra on (pg/mL)
Table 1. Calibration Curve no. 1 Table 2. Calibration Curve no. 4
Concentration Response Concentratio Response
Sample ID Area Ratio Sample ID Area Ratio
(pg/mL) Factor n (pg/mL) Factor
Plasma C1 5 0.0229 0.00459 Plasma C1 5 0.0227 0.0045
Plasma C2 10 0.0468 0.00468 Plasma C2 10 0.0477 0.0048
Plasma C3 25 0.1130 0.00452 Plasma C3 25 0.1117 0.0045
Plasma C4 50 0.2162 0.00432 Plasma C4 50 0.2144 0.0043
Plasma C5 100 0.4556 0.00456 Plasma C5 100 0.4462 0.0045
Plasma C6 200 0.8706 0.00435 Plasma C6 200 0.8549 0.0043
Plasma C7 400 1.7639 0.00441 Plasma C7 400 1.7819 0.0045
Plasma C8 500 2.2001 0.00440 Plasma C8 500 2.1869 0.0044
Plasma C9 750 3.5731 0.00476 Plasma C9 750 3.5756 0.0048
Plasma C10 1000 5.0905 0.00509 Plasma C10 1000 4.9443 0.0049
Plasma C11 1500 6.9408 0.00463 Plasma C11 1500 6.7704 0.0045
Picture of ion source after running 500 equine Plasma C12
Plasma C13
2000
2500
9.5574
11.6480
0.00478
0.00466
Plasma C12
Plasma C13
2000
2500
9.5426
11.8610
0.0048
0.0047
plasma samples and 4 calibration curves. Average 0.004596 Average 0.004568
Std. Dev 0.0002104 Std. Dev 0.0002111
%RSD of Response Factor 4.58% %RSD of Response Factor 4.62%
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

6. A Picture is Worth a Thousand Words
Picture of the EVOQ LC-TQ ion source after running 500
equine plasma samples and 4 calibration curves.
Game-Changing Performance
Stays cleaner maintains Sensitivity
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

7. Improved Dual Ion Funnel Design
Inter-laced Quadrupole Dual Ion Funnel
• Linear, on-axis design of the IQ dual ion funnel virtually eliminates
instrument-to-instrument tune variability.
• IQ dual ion funnel does not require DC; no impact on tuning
parameters after running hundreds of matrix rich samples.
• Less tuning, Less cleaning, Less maintenance equals more robustness
and more samples
User benefit provided by Bruker
• Effortlessly transfer methods between EVOQ instruments.
• Save time by eliminating re-tuning.
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

8. Sustained Sensitivity: 5-1000fg
Alprazolam in Rat Plasma On Column
On Column Resp.
Sample ID Area
(fg) Factor
5fg Alp 5 468 93.60
5fg Alp 5 487 97.40
5fg Alp 5 474 94.80
10fg Alp 10 981 98.10
10fg Alp 10 1083 108.30
10fg Alp 10 890 89.00
20fg Alp 20 2102 105.10
20fg Alp 20 2292 114.60
20fg Alp 20 2061 103.05
50fg Alp 50 4433 88.66
50fg Alp 50 4811 96.22
50fg Alp 50 4566 91.32
100fg Alp 100 9242 92.42 Full Calibration Range
100fg Alp 100 8295 82.95
100fg Alp 100 9441 94.41
200fg Alp 200 17934 89.67
200fg Alp 200 16800 84.00
200fg Alp 200 18136 90.68
500fg Alp 500 42485 84.97
500fg Alp 500 43900 87.80
500fg Alp 500 44571 89.14
1000fg Alp 1000 84747 84.75
Response Factor RSD (%) 8.75
EVOQ Elite with Advance HPLC
500uL/min LC flow rate
5-90% Acetonitrile (0.1% Formic) in 1.8min
C-18, 3u, 2.1x50, ACE column
2uL injected
5-50fg curve (n=3)
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

9. PACER Software allows Faster Review
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

10. See more on our Microsite
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

11. Game Changing Performance for the
Business of Quantitation
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.

12. Questions
Thank you
Chemical and Applied Markets
Copyright 2012 Bruker Corporation. All Rights Reserved.