Vitamin Analysis in Food and types of methods to do the analytics

1. Vitamin Analysis in
Food
Food Analysis
BTF201TC3
Group No 07

2. Contents
❖ What are Vitamins
❖ Important of vitamin analysis
❖ Type of vitamin analysis
❖ Bioassay method
❖ Microbiological assay method
❖ Physiochemical assay method

3. Group Members
❖ 2019/BST/031 Kavindu Ishan
❖ 2019/BST/032 Fathima Jahra
❖ 2019/BST/033 Ashen Gimantha
❖ 2019/BST/034 Shanika Madhuwanthi

4. What are vitamins??
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5. ❖ A vitamin is an organic molecule that is an
essential micronutrient which an organism
needs in small quantities for the proper
functioning of its metabolism..
❖ There are currently 13 recognized vitamins
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6. Fat-soluble vitamins
○ A - Retinol
○ D - Alfacalcidol
○ E - Tocopherol
○ K - Phytonadione
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7. Water-soluble vitamins
○ B-complex
○ C - Ascorbic acid
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8. Important of
Vitamin analysis
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9. ❖ Vitamin analysis is the determination of what
vitamins are present and at what levels in a
food, drink, raw material or ingredients
❖ It is usually carried out by sophisticated
chemical analysis such as chromatography
and mass spectrometry
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10. Benefits of Nutritional analysis
▰ Better quality check
▰ Increasing product consistency
▰ Easy Processing & Formulations
▰ Enables Better food fortification
▰ Reduced the incidence of food borne disease
▰ Easing marketing and distribution task
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11. Method of
analysis
Bioassay
method
Microbiological
assay methods
Physiochemical
assay method
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12. Biological Assay
Methods
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13. ❖ Extremely Time consuming
❖ Don’t necessary require preparation of an
extract
❖ Limited to animal rather than human
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14. 2 Type of Bioassay
methods
In vivo
bioassay
In vitro
bioassay
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15. Microbiological Assay
Methods
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16. ❖ The microbiological assay are better than
chemical method
❖ Microbiological assay is applicable only to the B
vitamins
❖ The microbiological assay of antibiotics and
vitamins were studied by using various strains
of microbes
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17. Physiochemical assay
methods
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18. A method of investigating physicochemical systems
that makes possible a determination of the nature of
the interactions between the components of a
system through a study of the relations between the
system's physical properties and composition.
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19. Number of physiochemical assay techniques used in
world
▰ Photometry ▰ Colorimetry ▰ Spectrophotometry
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20. Number of physiochemical assay
techniques used in world cont..
▰ Fluorimetry ▰ Flame Photometry
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21. Chromatography
❖ COLUMN CHROMATOGRAPHY
❖ PAPER CHROMATOGRAPHY
❖ THIN LAYER CHROMATOGRAPHY
❖ GAS CHROMATOGRAPHY
❖ HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
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22. Summarize….
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