What are the characteristics of each microscope 1.compound 2.stereo ( dissecting) 3. Phase contrast 4. Scanning (SEM) 5. Transmission (TEM) What are the characteristics of each microscope 1.compound 2.stereo ( dissecting) 3. Phase contrast 4. Scanning (SEM) 5. Transmission (TEM) 1.compound 2.stereo ( dissecting) 3. Phase contrast 4. Scanning (SEM) 5. Transmission (TEM) Solution The characteristics of each microscope is as follows: 1.COMPOUND MICROSCOPE In compound microscope the objective lens forms a real, inverted and magnified image of the object. The eye piece acts like a magnifying glass and forms a virtual erect and magnified image. Object is placed outside the principal focus of the objective lens. It consists of two convex lens, ocular, close to the eye and s3cond objective lens. It also reduces chromatic abberrations. 2. STEREO (DISSECTING) It is an optical microscope which is designed for low magnification, it uses light reflected from the surfacevof an object rather than transmitted through it. It forms three dimensional erect image as the prism under the eye piece reverses the image. 3. PHASE CONTRAST It is used to examine biological tissues (live microorganisms). It is a type of light microscopy that increases contrasts of transparent and colourless objects. This is achieved by influencing the optical path of the light. Thus contrast of light and dark makes the specimen visible to the human eye. 4. SCANNING (SEM). It is used for producing high resolution images of a sample surface. The images have 3-D appearance and best used to judge the surface structure of the sample. It produces image using focused beam of electrons. In SEM, the picture is shown on the monitor. 5.TRANSMISSION (TEM). It is based on transmitted electrons. It provides details of internal composition (show morphology, crystallisation, stress or even magnetic domains of the sample). In TEM, picture is shown on fluorescent screens. It provides 2-D picture of the sample. It uses electromagnetic lenses to focus the electrons into very thin beams..