1. The reaction of NEM with sulfhydryl groups in the oxy Hb tells that the Cys are in the reduced form while in the deoxy Hb the Cys are in the oxidized forms due to which they cannot react. 4. The data clearly suggest that NEM reacts more with oxy Hb and addition of NOS inhibitor drastically decrease the formation of SNO-Hb, while the NEM reaction with deoxy Hb is very low. Solution 1. The reaction of NEM with sulfhydryl groups in the oxy Hb tells that the Cys are in the reduced form while in the deoxy Hb the Cys are in the oxidized forms due to which they cannot react. 4. The data clearly suggest that NEM reacts more with oxy Hb and addition of NOS inhibitor drastically decrease the formation of SNO-Hb, while the NEM reaction with deoxy Hb is very low..