1. Microscopy
Applications of
in food research
Non-Thermal Processing Laboratory
Indian Institute of Technology
Kharagpur
Visit us: https://www.myprotocol.net/Laboratory/
2. Objectives of discussion
• Understand the need for microscopy
• Approaches to microscopy
• Methods of microscopy
• Scanning Electron Microscopy (SEM)
• Advantage and disadvantage of SEM
• Practical experience
4. Magnification
• It is a measure of how much larger a microscope
(or set of lenses within a microscope) causes an
object to appear.
5. Resolution
• The resolution of a microscope or lens is the
smallest distance by which two points can be
separated and still be distinguished as separate
objects.
14. Record
• Introduction, basic principle, working, limitation
1. AFM
2. TEM
3. SEM
• Advantage-disadvantages of SEM over TEM and
AFM
• Practical experience