5. SOURCES OF
CHOLESTEROL
Diet De novo synthesis
Cholesterol synthesized
in extrahepatic tissues
Liver cholesterol
pool
Free cholesterol
In bile
Conversion to bile salts/acidsSecretion of HDL
and VLDL
8. DIETARY CHOLESTEROL
Assume 400 mg intake /day
2 0 0 mg isabsorbed
1000 mg isexcreted
8 0 0 mg from de novosynthesis
Lowering cholesterol in diet has very little effect on
blood cholesterol !!!
9. Cholesterol Synthesis
Similar to ketogenic pathway
Occurs in cytosol
Requires NADPH and ATP
Highly regulated
80 % in liver, ~10% intestine, ~5% skin
11. HMG CoA Reductase :
Regulation
Short-term regulation
Inhibited byphosphorylation
AMP-dependent protein kinase
When AMP is high (or ATP low!), why waste energy on
cholesterol synthesis
Long-term regulation
Proteolysis
Degradation stimulated by cholesterol and cholesterol
metabolites
Sterol-sensing domain
12. HMG COA REDUCTASE -
Phosphorylation
AMP-Activated
Protein Kinase
(low activity)
phosphatase
insulin
(+)
kinase
Glucagon/epi
(+)
increase cAMP
AMP
AMP-Activated
Protein Kinase (high activity)
(+)
HMG CoA reductase – OH
(active)
HMG CoA reductase – P
(inactive)
13. HMG CoA Reductase :
Regulation
Transcriptional regulation
SREBP-2
Responds to cellular levels of sterols
20. LOWERING A
CHOLESTEROL
O a t bran
Soluble fiber
Bind bileacids?
Niacin
3-8 grams / day (need 60mg)
Inhibits VLDL excretion ? (fattyliver)
Flushing and liverdamage