leading and lagging strand DNA polymerase conclusion

1. CONCURRENTSYNTHESISONTHE
LEADING ANDLAGGINGSTRANDS

2. LEADINGAND LAGGING STRAND
The parent strand that runs 5’-3’ in the reverse direction of
fork movement is termed the leading strand ,and it serves as a
template for the continuous DNA synthesis.
The other parent strand that runs 5’-3’ in the direction of fork
movement is termed the lagging strand , and it serves as a
template for the discontinuous strand .

3. DNAPolymerase III
 It is a haloenzyme consist of a dimer containing 10 different
polypeptide unit.
This haloenzyme is main polymerase of E. coil.
It is made up of three assemblies :
• Clamp loading complex
• B sliding clamp processivity factor
• Core enzyme

4. Fig. showing the concurrent DNAsynthesis on the leading and the lagging strand

5. CONCLUSION
• Concurrent DNA may be achieved on both the leading and
lagging strand at a single replication fork .
• The lagging template strand is “looped” in order to invert
the physical direction of synthesis , but not the biochemical
direction.
• The enzyme functions as a dimer with each core enzyme
achieving synthesis on one or the other strand.

6. THANKYOU