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AJAY
KUMAR
GAUTAM
•The agricultural practice of breeding and raising livestock.
•Deals with care and breeding of livestock like buffaloes, cows, pigs, horses,
cattle, sheep, camels, goats etc. Extended form includes poultry farming and
fisheries.
•Fisheries include rearing, catching,selling etc. of fish, mollusks (shell fish) and
crustaceans (prawns, crabs etc.)
•Since time immemorial, animals like bees, silk-worm, prawns, crabs, fishes,
birds, pigs, cattle, sheep and camels have been used by humans for products like
milk, eggs, meat, wool, silk, honey, etc.
•It is estimated that more then 70 per cent of the world livestock population is in
India and China. However, it is surprising to note that the contribution to the
world farm produce is only 25 per cent, i.e., the productivity per unit is very low.
•Hence, in addition to conventional practices of animal breeding and care, newer
technologies also have to be applied to achieve improvement in quality and
productivity.
ANIMAL HUSBANDRY
Management of Farms and Farm
Animals
Dairy Farm
Management
Poultry Farm
Management
Bee Keeping
(Apiculture)
Fisheries
•Dairying is the management of animals for milk and its
products for human consumption. In dairy farm management,
we deal with processes and systems that increase yield and
improve quality of milk.
•Selection of good breeds having high yielding potential,
combined with resistance to diseases is very important, such as
Cattle have to be housed well, should have adequate water and
be maintained disease free.
•The feeding of cattle should be carried out in a scientific
manner (quality and quantity of fodder).
•Stringent cleanliness and hygiene are importance while
milking, storage and transport of the milk and its products.
•Regular visit by a veterinary doctor would be mandatory.
Dairy Farm Management
• Poultry is the class of
domesticated fowl used for
food or for their eggs.
• Selection of disease free and
suitable breeds, proper and safe
farm conditions, proper feed
and water, and hygiene and
health care are important
components of poultry farm
management.
Poultry Farm
Management
• It is the maintenance of a beehive
of honey bees for the production of
honey. There are several species of
honeybees which can be reared.
• Apis indica is the most common
species of honey bees.
• Honey is a food of high nutritive
value and also finds use in the
indigenous systems of medicine.
•Honeybee also produces beeswax,
which finds many uses in industry,
such as in the preparation of
cosmetics and polishes of various
kinds.
The following points are important for successful
bee-keeping.
i. Knowledge of the nature and habits of bees
ii. Selection of suitable location for keeping the
beehives,
iii. Catching and hiving of swarms (group of bees),
iv. Management of beehives during different
seasons,
v. Handling and collection of honey and of
beeswax. Bees are the pollinators of many of our
crop species such as sunflower, Brassica, apple
and pear.
vi. Keeping beehives in crop fields during flowering
period increases pollination efficiency and
improves the yield–beneficial both from the poin
of view of crop yield and honey yield.
FISHERIES
• Fishery is an industry devoted to the catching,
processing or selling of fish, shellfish or other aquatic
animals.
• Some of the freshwater fishes which are very common
include Catla, Rohu and common carp. Some of the
marine fishes that are eaten include – Hilsa, Sardines,
Mackerel and Pomfrets.
• Fisheries has an important place in Indian economy. It
provides income and employment to millions of
fishermen and farmers, particularly in the coastal states.
• In order to meet the increasing demands on fisheries,
different techniques have been employed to increase
production. For example, through aquaculture and
pisciculture we have been able to increase the
production of aquatic plants and animals, both fresh-
water and marine.
• This has led to the development and flourishing of the
fishery industry, and it has brought a lot of income to the
farmers in particular and the country in general.
•A group of animals related by descent and similar in most
characters like general appearance, features size, configuration,
etc, are said to be a breed.
•Animal breeding aims at increasing the yield of animals and
improving the desirable qualities of the produce.
•When breeding is between animals of the same breed it is
called Inbreeding, while crosses between different breeds are
called Outbreeding.
Animal Breeding
Breeding
Inbreeding
Outbreeding
Out Crossing
Cross Breeding
Interspecific
Hybridization
• It refers to the mating of more
closely related individuals within the
same breed for 4-6 generations.The
breeding strategy is, superior males
and superior females of the same
breed are identified and mated in
pairs.
• Inbreeding increases
Homozygosity. However, continued
inbreeding, especially close
inbreeding, usually reduces fertility
and even productivity.This is called
Inbreeding Depression.
• Whenever this becomes a problem,
selected animals of the breeding
population should be mated with
unrelated superior animals of the
same breed.This usually helps
restore fertility and yield.
Inbreeding Out-breeding
• It is the breeding of the
unrelated animals,
which may be between
individuals of the
different breeds or
different species.
• Types of Outbreeding :
a) Outcrossing
b) Cross Breeding
c) Interspecific
Hybridization
Artificial Insemination
•The semen is collected from the male that is chosen
as a parent and injected into the reproductive tract of
the selected female by the breeder.
•The semen may be used immediately or can be
frozen and used at a later date.
•It can also be transported in a frozen form to where
the female is housed.
•In this way desirable matings are carried.
•Artificial insemination helps us overcome several
problems of normal matings.
Controlled Breeding Experiments
•Programme for herd improvement.
•In this method, a cow is administered hormones, with FSH-like activity, to
induce follicular maturation and super ovulation – instead of one egg,
which they normally yield per cycle, they produce 6-8 eggs.
•The animal is either mated with an elite bull or artificially inseminated.
The fertilised eggs at 8–32 cells stages, are recovered non-surgically and
transferred to surrogate mothers.
•The genetic mother is available for another round of super ovulation. This
technology has been demonstrated for cattle, sheep, rabbits, buffaloes,
mares, etc.
•High milk-yielding breeds of females and high quality (lean meat with less
lipid) meat-yielding bulls have been bred successfully to increase herd size
in a short time.
Multiple Ovulation Embryo
Transfer Technology (MOET)
PLANT BREEDING
 Plant breeding is the purposeful manipulation of
plant species in order to create desired plant
types that are better suited for cultivation.
 These plants gives better yields and are disease
resistant.
 Green revolution was dependent to a large
extent on plant breeding techniques for
development of high–yielding and disease
resistant crops.
 Several varieties of wheat, rice, maize are
developed by the help of plant breeding
techniques.
1. Collection of variability – Collection and preservation of all
the different wild varieties, species and relatives of the
cultivated species.
2. Evaluation and selection of parents – Evaluation is done to
identify plants with desirable characters. The selected plants
are multiplied and used in the process of hybridization.
3. Cross hybridization among the selected parents – By cross
hybridizing the two parents to produce hybrids that
genetically combine the desired characters in one plant.. Cross
hybridization is done by Emasculation and bagging.
4. Selection and testing of superior recombinants – The
selection process is crucial to the success of the breeding
objective and requires careful scientific evaluation of the
progeny. These are self pollinated for several generations till
they reach a state of uniformity, so that the characters will not
segregate in the progeny.
5. Testing, release and commercialization of new cultivars –
This evaluation is done by growing these plants in the
research fields and recording their performance under ideal
fertilizer application irrigation, and other crop management
practices. It will be followed by testing the materials in
farmers’ fields, for at least three growing at several locations
in the country.
.
Wheat & Rice
 In 1963, several varieties such as Sonalika and
Kalyan Sona, which were high yielding and
disease resistant, were introduced all over the
wheat-growing belt of India.
 Semi-dwarf rice varieties were derived from IR-
8, and Taichung Native-1 were introduced in
1966.
 Later better-yielding semi-dwarf varieties Jaya
and Ratna were developed in India.
 Semi dwarf varieties was developed by Norman
E Borlaug in Mexico.
SUGARCANE
 Saccharum barberi, grown in
north India, had poor sugar
content and yield.
 Saccharum officinarum, grown
in South India, had thicker stem
and higher sugar content. But
they did not grow in North
India.
 The sugarcane species
obtained after cross breeding
between these two species, had
thick stems, high sugar
content, high yield and ability
to grow in North India also .
Plant Breeding for Disease
Resistance
 Screening germplasm for resistance - Germplasm is the sum
total of all the alleles of the genes present in a crop and its
related species.
 Hybridization of selected parents - Hybridization is the most
common method of creating genetic variation. Hybridization is
crossing of two or more types of plants for bringing their traits
together in the progeny.
 Selection and evaluation of the hybrids - Selection of parents is
picking up seeds of only those plants for multiplication which
have the desired traits. For example, grain length in rice is
variable— longer grains, intermediate grains and shorter grains.
 Testing and release of new varieties - The newly selected lines
are evaluated for their yield and other agronomic traits of quality,
disease resistance, etc.This evaluation is done by growing these
in the research field and recording their performance under ideal
fertilizer (application), irrigation, etc.
CROP VARIETY RESISTANCE TO
DISEASE
Wheat Himgiri Leaf and stripe rust, hill
bunt
Brassica Pusa swarnim (Karan
rai)
White rust
Cauliflower Pusa shubhra, Pusa
snowball K-1
Black Rot and Curl
blight black rot
Cowpea Pusa Komal Bacterial Blight
Chilli Pusa Sadabahar Chiily mosiac virus,
Tobaco mosiac virus
and Leaf Curl
Plant Breeding for Disease
Resistance
Mutation Breeding
 It is the process by which genetic
variations are created through changes
in the base sequence within genes
resulting in the creation of a new
character or trait not found in the
parental type.
 It is possible to induce mutations
artificially through use of chemicals or
radiations, and selecting and using the
plants that have the desirable character
as a source in breeding.
 For example, in moong bean, resistance
to yellow mosaic virus and powdery
mildew were induced by mutations.
 Resistance to yellow mosaic virus in
bhindi (Abelmoschus esculentus) was
transferred from a wild species and
resulted in a new variety of A.
esculentus called Parbhani kranti
Plant Breeding for Developing Resistance
to Insects Pests
 Insect resistance in host crop plants may be due to
morphological, biochemical or physiological
characteristics.
 Hairy leaves in several plants are associated with
resistance to insect pests, e.g., resistance to jassids
in cotton and cereal leaf beetle in wheat.
 High aspartic acid, low nitrogen and sugar content
in maize leads to resistance to maize stem borers.
 Smooth leaved and nectar-less cotton varieties do
not attract bollworms.
CROP VARIETY INSECTS
PESTS
Brassica
(rapeseed mustard)
Pusa Gaurav Aphids
Flat Bean
Pusa Sem 2,
Pusa Sem 3
Jassids, aphids and
Fruit borer
Okra(Bhindi) Pusa Sawani
Pusa A-4
Shoot and Fruit borer
Plant Breeding for Developing Resistance to
Insects Pests
Plant Breeding for Improved Food
Quality
 Diets lacking essential micronutrients – particularly
iron, vitamin A, iodine and zinc –increase the risk for
disease, reduce lifespan and reduce mental abilities.
 A far greater number–three billion people – suffer
from micronutrient, protein and vitamin deficiencies
or ‘hidden hunger’ because they cannot afford to buy
enough fruits, vegetables, legumes, fish and meat.
 More than 840 million people in the world do not
have adequate food to meet their daily food and
nutritional requirements.
Bio Fortification
 It is the process of breeding crops with higher levels of vitamins and minerals,
or higher protein and healthier fats.
 Breeding for improved nutritional quality is undertaken with the objectives of
improving :
 Protein content and quality
 Oil content and quality
 Vitamin content ; and
 Micronutrient and mineral content
 Examples :
 Vitamin A enriched carrots, spinach, pumpkin
 Vitamin C enriched bitter gourd, mustard, tomato
 Iron and Calcium enriched spinach and Protein enriched Beans.
 The Indian Agricultural Research Institute, New Delhi
Crop Varieties With Increased Nutritional
Quality
 Lysine and tryptophan rich maize
 High protein rich wheat (Atlas 66)
 Iron fortified rice
 Vitamin C rich bitter gourd, tomato,
mustard
 Iron and calcium rich spinach and bathua
 Vitamin A rich carrots, spinach and
pumpkin.
TISSUE CULTURE
•Plant tissue culture refers to the maintenance and growth of plant
cells, tissues and organs on a suitable synthetic medium in vitro and
the whole plants could be regenerated from explants.
•Explant : An explant is the plant part excised from a specific
location in a plant, to be used for initiating a culture.
•In this tissue culture process, explant, i.e., any part of a plant is taken
out and grown in a test tube, under sterile conditions in special
nutrient media.
•This capacity to generate a whole plant from any cell / explant is
called Totipotency.
•This method of producing thousands of plants through tissue culture
is called Micro propagation.
•Each of these plants will be genetically identical to the original plant
from which they were grown i.e., they are soma clones.
TISSUE CULTURE
Components That Are Used In The
Tissue Culture Nutrient Medium Are
1. Distilled water
2. Inorganic salts
3. Vitamins
4. Amino acids
5. Growth hormones
6. Sucrose
7. Agar
• Production of large number of plant from small tissue or single
cell. Production of genetically identical plants (somaclones).
•Recovery of healthy plants from diseased plants by meristem
culture.
•Seedless plants can be multiplied
Applications Of Plant Tissue
Culture
•The first step in somatic hybridization is to remove the cell
wall by digesting it with enzymes like pectinase and cellulase.
•Isolated protoplasts from two different varieties of plants –
each having a desirable character – can be fused to get hybrid
protoplasts, which can be further grown to form a new plant.
•These hybrids are called somatic hybrids while the process is
called somatic hybridization.
•For example, a protoplast of tomato is fused with that of potato
to form new hybrid plants combining tomato and potato
characteristics.
Somatic Hybridisation/Somatic
Hybrids:

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ANIMAL HUSBANDRY AND PLANT BREEDING

  • 2. •The agricultural practice of breeding and raising livestock. •Deals with care and breeding of livestock like buffaloes, cows, pigs, horses, cattle, sheep, camels, goats etc. Extended form includes poultry farming and fisheries. •Fisheries include rearing, catching,selling etc. of fish, mollusks (shell fish) and crustaceans (prawns, crabs etc.) •Since time immemorial, animals like bees, silk-worm, prawns, crabs, fishes, birds, pigs, cattle, sheep and camels have been used by humans for products like milk, eggs, meat, wool, silk, honey, etc. •It is estimated that more then 70 per cent of the world livestock population is in India and China. However, it is surprising to note that the contribution to the world farm produce is only 25 per cent, i.e., the productivity per unit is very low. •Hence, in addition to conventional practices of animal breeding and care, newer technologies also have to be applied to achieve improvement in quality and productivity. ANIMAL HUSBANDRY
  • 3. Management of Farms and Farm Animals Dairy Farm Management Poultry Farm Management Bee Keeping (Apiculture) Fisheries
  • 4. •Dairying is the management of animals for milk and its products for human consumption. In dairy farm management, we deal with processes and systems that increase yield and improve quality of milk. •Selection of good breeds having high yielding potential, combined with resistance to diseases is very important, such as Cattle have to be housed well, should have adequate water and be maintained disease free. •The feeding of cattle should be carried out in a scientific manner (quality and quantity of fodder). •Stringent cleanliness and hygiene are importance while milking, storage and transport of the milk and its products. •Regular visit by a veterinary doctor would be mandatory. Dairy Farm Management
  • 5. • Poultry is the class of domesticated fowl used for food or for their eggs. • Selection of disease free and suitable breeds, proper and safe farm conditions, proper feed and water, and hygiene and health care are important components of poultry farm management. Poultry Farm Management
  • 6. • It is the maintenance of a beehive of honey bees for the production of honey. There are several species of honeybees which can be reared. • Apis indica is the most common species of honey bees. • Honey is a food of high nutritive value and also finds use in the indigenous systems of medicine. •Honeybee also produces beeswax, which finds many uses in industry, such as in the preparation of cosmetics and polishes of various kinds.
  • 7. The following points are important for successful bee-keeping. i. Knowledge of the nature and habits of bees ii. Selection of suitable location for keeping the beehives, iii. Catching and hiving of swarms (group of bees), iv. Management of beehives during different seasons, v. Handling and collection of honey and of beeswax. Bees are the pollinators of many of our crop species such as sunflower, Brassica, apple and pear. vi. Keeping beehives in crop fields during flowering period increases pollination efficiency and improves the yield–beneficial both from the poin of view of crop yield and honey yield.
  • 8. FISHERIES • Fishery is an industry devoted to the catching, processing or selling of fish, shellfish or other aquatic animals. • Some of the freshwater fishes which are very common include Catla, Rohu and common carp. Some of the marine fishes that are eaten include – Hilsa, Sardines, Mackerel and Pomfrets. • Fisheries has an important place in Indian economy. It provides income and employment to millions of fishermen and farmers, particularly in the coastal states. • In order to meet the increasing demands on fisheries, different techniques have been employed to increase production. For example, through aquaculture and pisciculture we have been able to increase the production of aquatic plants and animals, both fresh- water and marine. • This has led to the development and flourishing of the fishery industry, and it has brought a lot of income to the farmers in particular and the country in general.
  • 9.
  • 10. •A group of animals related by descent and similar in most characters like general appearance, features size, configuration, etc, are said to be a breed. •Animal breeding aims at increasing the yield of animals and improving the desirable qualities of the produce. •When breeding is between animals of the same breed it is called Inbreeding, while crosses between different breeds are called Outbreeding. Animal Breeding
  • 12. • It refers to the mating of more closely related individuals within the same breed for 4-6 generations.The breeding strategy is, superior males and superior females of the same breed are identified and mated in pairs. • Inbreeding increases Homozygosity. However, continued inbreeding, especially close inbreeding, usually reduces fertility and even productivity.This is called Inbreeding Depression. • Whenever this becomes a problem, selected animals of the breeding population should be mated with unrelated superior animals of the same breed.This usually helps restore fertility and yield. Inbreeding Out-breeding • It is the breeding of the unrelated animals, which may be between individuals of the different breeds or different species. • Types of Outbreeding : a) Outcrossing b) Cross Breeding c) Interspecific Hybridization
  • 13. Artificial Insemination •The semen is collected from the male that is chosen as a parent and injected into the reproductive tract of the selected female by the breeder. •The semen may be used immediately or can be frozen and used at a later date. •It can also be transported in a frozen form to where the female is housed. •In this way desirable matings are carried. •Artificial insemination helps us overcome several problems of normal matings. Controlled Breeding Experiments
  • 14. •Programme for herd improvement. •In this method, a cow is administered hormones, with FSH-like activity, to induce follicular maturation and super ovulation – instead of one egg, which they normally yield per cycle, they produce 6-8 eggs. •The animal is either mated with an elite bull or artificially inseminated. The fertilised eggs at 8–32 cells stages, are recovered non-surgically and transferred to surrogate mothers. •The genetic mother is available for another round of super ovulation. This technology has been demonstrated for cattle, sheep, rabbits, buffaloes, mares, etc. •High milk-yielding breeds of females and high quality (lean meat with less lipid) meat-yielding bulls have been bred successfully to increase herd size in a short time. Multiple Ovulation Embryo Transfer Technology (MOET)
  • 15.
  • 16. PLANT BREEDING  Plant breeding is the purposeful manipulation of plant species in order to create desired plant types that are better suited for cultivation.  These plants gives better yields and are disease resistant.  Green revolution was dependent to a large extent on plant breeding techniques for development of high–yielding and disease resistant crops.  Several varieties of wheat, rice, maize are developed by the help of plant breeding techniques.
  • 17.
  • 18. 1. Collection of variability – Collection and preservation of all the different wild varieties, species and relatives of the cultivated species. 2. Evaluation and selection of parents – Evaluation is done to identify plants with desirable characters. The selected plants are multiplied and used in the process of hybridization. 3. Cross hybridization among the selected parents – By cross hybridizing the two parents to produce hybrids that genetically combine the desired characters in one plant.. Cross hybridization is done by Emasculation and bagging. 4. Selection and testing of superior recombinants – The selection process is crucial to the success of the breeding objective and requires careful scientific evaluation of the progeny. These are self pollinated for several generations till they reach a state of uniformity, so that the characters will not segregate in the progeny. 5. Testing, release and commercialization of new cultivars – This evaluation is done by growing these plants in the research fields and recording their performance under ideal fertilizer application irrigation, and other crop management practices. It will be followed by testing the materials in farmers’ fields, for at least three growing at several locations in the country.
  • 19. .
  • 20. Wheat & Rice  In 1963, several varieties such as Sonalika and Kalyan Sona, which were high yielding and disease resistant, were introduced all over the wheat-growing belt of India.  Semi-dwarf rice varieties were derived from IR- 8, and Taichung Native-1 were introduced in 1966.  Later better-yielding semi-dwarf varieties Jaya and Ratna were developed in India.  Semi dwarf varieties was developed by Norman E Borlaug in Mexico.
  • 21. SUGARCANE  Saccharum barberi, grown in north India, had poor sugar content and yield.  Saccharum officinarum, grown in South India, had thicker stem and higher sugar content. But they did not grow in North India.  The sugarcane species obtained after cross breeding between these two species, had thick stems, high sugar content, high yield and ability to grow in North India also .
  • 22. Plant Breeding for Disease Resistance  Screening germplasm for resistance - Germplasm is the sum total of all the alleles of the genes present in a crop and its related species.  Hybridization of selected parents - Hybridization is the most common method of creating genetic variation. Hybridization is crossing of two or more types of plants for bringing their traits together in the progeny.  Selection and evaluation of the hybrids - Selection of parents is picking up seeds of only those plants for multiplication which have the desired traits. For example, grain length in rice is variable— longer grains, intermediate grains and shorter grains.  Testing and release of new varieties - The newly selected lines are evaluated for their yield and other agronomic traits of quality, disease resistance, etc.This evaluation is done by growing these in the research field and recording their performance under ideal fertilizer (application), irrigation, etc.
  • 23. CROP VARIETY RESISTANCE TO DISEASE Wheat Himgiri Leaf and stripe rust, hill bunt Brassica Pusa swarnim (Karan rai) White rust Cauliflower Pusa shubhra, Pusa snowball K-1 Black Rot and Curl blight black rot Cowpea Pusa Komal Bacterial Blight Chilli Pusa Sadabahar Chiily mosiac virus, Tobaco mosiac virus and Leaf Curl Plant Breeding for Disease Resistance
  • 24. Mutation Breeding  It is the process by which genetic variations are created through changes in the base sequence within genes resulting in the creation of a new character or trait not found in the parental type.  It is possible to induce mutations artificially through use of chemicals or radiations, and selecting and using the plants that have the desirable character as a source in breeding.  For example, in moong bean, resistance to yellow mosaic virus and powdery mildew were induced by mutations.  Resistance to yellow mosaic virus in bhindi (Abelmoschus esculentus) was transferred from a wild species and resulted in a new variety of A. esculentus called Parbhani kranti
  • 25. Plant Breeding for Developing Resistance to Insects Pests  Insect resistance in host crop plants may be due to morphological, biochemical or physiological characteristics.  Hairy leaves in several plants are associated with resistance to insect pests, e.g., resistance to jassids in cotton and cereal leaf beetle in wheat.  High aspartic acid, low nitrogen and sugar content in maize leads to resistance to maize stem borers.  Smooth leaved and nectar-less cotton varieties do not attract bollworms.
  • 26. CROP VARIETY INSECTS PESTS Brassica (rapeseed mustard) Pusa Gaurav Aphids Flat Bean Pusa Sem 2, Pusa Sem 3 Jassids, aphids and Fruit borer Okra(Bhindi) Pusa Sawani Pusa A-4 Shoot and Fruit borer Plant Breeding for Developing Resistance to Insects Pests
  • 27. Plant Breeding for Improved Food Quality  Diets lacking essential micronutrients – particularly iron, vitamin A, iodine and zinc –increase the risk for disease, reduce lifespan and reduce mental abilities.  A far greater number–three billion people – suffer from micronutrient, protein and vitamin deficiencies or ‘hidden hunger’ because they cannot afford to buy enough fruits, vegetables, legumes, fish and meat.  More than 840 million people in the world do not have adequate food to meet their daily food and nutritional requirements.
  • 28. Bio Fortification  It is the process of breeding crops with higher levels of vitamins and minerals, or higher protein and healthier fats.  Breeding for improved nutritional quality is undertaken with the objectives of improving :  Protein content and quality  Oil content and quality  Vitamin content ; and  Micronutrient and mineral content  Examples :  Vitamin A enriched carrots, spinach, pumpkin  Vitamin C enriched bitter gourd, mustard, tomato  Iron and Calcium enriched spinach and Protein enriched Beans.  The Indian Agricultural Research Institute, New Delhi
  • 29. Crop Varieties With Increased Nutritional Quality  Lysine and tryptophan rich maize  High protein rich wheat (Atlas 66)  Iron fortified rice  Vitamin C rich bitter gourd, tomato, mustard  Iron and calcium rich spinach and bathua  Vitamin A rich carrots, spinach and pumpkin.
  • 30.
  • 31. TISSUE CULTURE •Plant tissue culture refers to the maintenance and growth of plant cells, tissues and organs on a suitable synthetic medium in vitro and the whole plants could be regenerated from explants. •Explant : An explant is the plant part excised from a specific location in a plant, to be used for initiating a culture. •In this tissue culture process, explant, i.e., any part of a plant is taken out and grown in a test tube, under sterile conditions in special nutrient media. •This capacity to generate a whole plant from any cell / explant is called Totipotency. •This method of producing thousands of plants through tissue culture is called Micro propagation. •Each of these plants will be genetically identical to the original plant from which they were grown i.e., they are soma clones.
  • 33.
  • 34. Components That Are Used In The Tissue Culture Nutrient Medium Are 1. Distilled water 2. Inorganic salts 3. Vitamins 4. Amino acids 5. Growth hormones 6. Sucrose 7. Agar
  • 35. • Production of large number of plant from small tissue or single cell. Production of genetically identical plants (somaclones). •Recovery of healthy plants from diseased plants by meristem culture. •Seedless plants can be multiplied Applications Of Plant Tissue Culture
  • 36. •The first step in somatic hybridization is to remove the cell wall by digesting it with enzymes like pectinase and cellulase. •Isolated protoplasts from two different varieties of plants – each having a desirable character – can be fused to get hybrid protoplasts, which can be further grown to form a new plant. •These hybrids are called somatic hybrids while the process is called somatic hybridization. •For example, a protoplast of tomato is fused with that of potato to form new hybrid plants combining tomato and potato characteristics. Somatic Hybridisation/Somatic Hybrids: