The document summarizes research into developing a nano-emulsion formulation of the NSAID mefenamic acid. Key points: - The goal was to improve the drug's efficacy, stability, and permeability for treating rheumatoid arthritis. Capmul MCM oil, tween 60 surfactant, and PEG co-surfactant were selected. - Spontaneous emulsification was used to construct phase diagrams and optimize ratios of oil, surfactant, and co-surfactant. The most stable formulation showed clarity by visual examination and transmittance testing. - Testing under various stress conditions like centrifugation, heating/cooling, and freeze/thaw cycles demonstrated the formulation's physical

1. Formulation and evaluation of nano-emulsion of NSAID
(mefenamic acid)
Reference –formulation and evaluation of nano emulsion for NSAID Gajanan M. Vaibhav C IJSR 2018
Page no- 169-172
Prepared by- Guided by-
Adinath Sangale Dr.Pandhare R.B
M pharm 1st year Mpharm PhD
Roll no- 10
Mula Education Society’s College Of Pharmacy, Sonai Ahmednagar
2021-022
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2. 2

3.  Content
• Abstract
• Introduction
• Materials
• Methods
• Screening of component
• Result and Discussion
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4.  ABSTRACT
Aim to develope nano-emulsion of mefenamic acid for
improving its efficacy stability & permeability for treating RA.
Prepared by using capmul oil as oil phase tween 60 as
surfactant PEG as co surfactant.
This formulation were subjected to various physical stability
test like centrifugation test heating coolling stress
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5.  INTRODUCTION
 Rheumatoid arithritist a autoimmune disease causing inflammation to
the synovial joints
Mefenamic acid is the newest member of anthraliinic acid class of NSAID
Mechanism of action- interferes the synthesis of prostaglandins from
arachidonic acid
Main purpose of this formulation to overcome the drawback of dosage from
& improve efficacy of drug
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6. MATERIALS
• Mefenamic acid
• Tween series surfactants
• PEG
• Co surfactants
• Capmul MCM
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7.  METHOD
• 1 Formulation development & optimization
• 1.1 Authentication of excipients
 Authentication of oils surfactants & co surfactants used for formation of nano
emulsion was checked by determining their oraganoleptic properties &MP
determination etc.
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8. 1.2 Screening of component
In this solublity of mefenamic acid in different oils surfactants &
cosurfactants is determined
Drug added to 1ml of selected oil surfactant & cosurfactant in 5ml of vials
Mixing using vortex mixer & then kept for 72 hrs. at 25°C in isothermal
condition shake to reach equilibrium
Centrifugated at 3000rpm for 15 min& filtered through 0.45 micrometer
Analyzed in uv at 286 nm
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9. Solublity of mefenamic acid in different component
Drug having highest solubilty in the capmul MCM oil & tween 60 and PEG 400
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10. 1.3 Spontaneous emulsification method
To construct the phase diagram surfactant was mixed with co
surfactant in different proportion (1:2 2:1 3:1 1:2 1:3 ) with increasing
concentration of surfactant to co surfactant
The selected ratio of Smix further treated with oil different ratios (1:9
2:8 3:7 4:6 1:2 1:2 1:3:5 1:5 1:6 1:7 1:8 )
Prepared nano emulsion were placed in tightly closed glass vails &
stored in ambient tempreture.
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11.  Centrifugation test
• Formulation were centrifugated at the 3000 a& 4000 rpm for 30 min &
observed for phase sepration cracking or creaming
Heating and cooling cycle
• To observe the stressed effect on formulation
• Kept at 45°C & 0°C for NLT 48 hrs.
Freeze & thaw cycle (accelerated aging )
• For accelerated stability testing
• Formulation expressed at two different tempt -21°C& 21°C cycle NMT
24hrs.
• Six cycle should be run at each batch 11

12.  Appearance
• By visual examination
• Turbidity , transpearancy checked
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13.  % Transmittance
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• It checked against distilled H2O in uv at 650 nm
• In case of system having %T value less than 95% suggest the clarity of
emulsion this may due to greater droplet size of the formation

14.  Refractive index
• Its an optical property used to characterize the isotrpic in nature of nanoemulsion
• RI determined by Abbes type
• RI in the range of 1.351 to 1.393 this indicates RI value increases with concentration of
oil & with decreasing aqueous concentration
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15.  Electrical conductivity
• Type of nano emulsion (O/W W/O) & the Stability of nano emulsion is determined by
electrical conductivity
• Using digital conductometry
• The given formulation was O/W type nano emulsion because water is continuous
phase
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16.  Result and discussion
• Clarity of nano emulsion was checked by transparency and measured
in term of transmittance
• Formulation was checked for transparency to turbidity it remained
clear slightly yellowish easily flowable formulation
• RI in the range of 1.351 to 1.393 this indicates RI value increases with
concentration of oil & with decreasing aqueous concentration
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17. Reference
• Gajanan M. Vaibhav C. formulation and evaluation of mefenamic acid
IJPRD 2018 pp 169-173
• Cesur S. Yylaci C. optimum kinetic parameter of mefenamic acid
crystallization by PBM JCPT, 2012 pp 81-95
• Oswal T naik S. formulation and evaluation of mefenamic acid emulgel
IJPRD 2014 5(12) pp 91-100
• Patel KC. Pramanik S. formulation and characterization of mefenamic
acid loaded polymeric nanoparcticle world J pharma sci 2014 pp-1391-
1405
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