2. INTRODUCTION
โข Listeria monocytogenes is a Gram positive intracellular bacteria having toxins called
as Listeriolysin-O (LLO),which is actually a cholesterol dependent cytolysin.
โข This bacterium causes Circling Disease which has got neuronal manifestations with
micro abscess in brain and lymphocytic Leptomeningitis.
โข The bacterium was named after Joseph Lister.
โข Not 100% accurate.
โข Tumbling motility at 25 degree centigrade is a feature of the organism.
3. Procedure
โข Neural tissue isolates are taken from the suspected animal.
โข Injected into conjunctival sac of rabbit or guinea pig.
โข If at all the lab animal is getting a severe keratoconjunctivitis, then the test
sample is considered as Positive for Listeriosis.