Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Virus neutralization test

ppt

  • Be the first to comment

Virus neutralization test

  1. 1. VIRUS NEUTRALIZATION TEST SHAREEFNGUNGUNI6/1/2017 1
  2. 2. VIRUS NEUTRALIZATION TEST Introduction  Serological method that detects the presence of viral neutralizing antibodies.  The antibodies bind to the viral particles • Monoclonal and polyclonal antibodies  Block viral infectivity-No cell infection SHAREEFNGUNGUNI6/1/2017 2
  3. 3. VNT cont..,  VNT are conducted by :  mixing dilutions of antibodies with standardized amount of virus, incubating them and cultured into cells, eggs or animals to have a clear cytopathic effect observation  CPE effects:  Rounding of cells  Change in texture-granular/hyaline/glassy  Formation of syncytium SHAREEFNGUNGUNI6/1/2017 3
  4. 4. VNT cont..,  STEPS • First step: • Serial dilutions of serum(virus-neutralizing Ab?)+ known virus • Incubated for 1-2hr at 37 degrees Celsius • Second step; • Cell culture inoculated with mixture • Seal the plates & Incubated at 37 degrees Celsius • Direct microscope SHAREEFNGUNGUNI6/1/2017 4
  5. 5. VNT cont., • Illustrations using diagrams Copyright 2009, John Wiley & Sons, Inc. Copyright 2009, John Wiley & Sons, Inc. Copyright 2009, John Wiley & Sons, Inc.. right 2009, John Wiley & Sons, Inc. 6/1/2017 5 SHAREEFNGUNGUNI
  6. 6. VNT cont.., • Expectations • No Ab(in serum)+virus no neutralization CPE • Ab(in serum)+virus neutralization No CPE • Cells remain intact SHAREEFNGUNGUNI6/1/2017 6
  7. 7. VNT cont.., -pro’s: • Can be specific • Sensitive • Vaccine production/immunological studies -con’s: • Very slow(days) • Intensive • Require skilled people • Depend on cell line-use of wrong cell lines may assume that the antibodies have neutralization ability when not, or may seem to be ineffective when they actually have the neutralization abilities. SHAREEFNGUNGUNI6/1/2017 7
  8. 8. Conclusion All in All, viral infections can be diagnosed in laboratory by many methods and the gold standard one is neutralization tests since it is a technique to which all other test methods have been compared with. Although the technique is highly sensitive and specific in detection of antibodies, it has disadvantages. SHAREEFNGUNGUNI6/1/2017 8
  9. 9. REFERENCES 1) Carter,J.,and Saunders,V.(2007).Virology:Principles and Applications.John Willey & Sons,Ltd.England,UK. 2) Flint,S.J.,Enquist,L.W.,Racaniello,V.R.,and Skalka,A.M.(2004).Principles of virology:Molecular Biology,Pathogenesis and Control of Animal Viruses.2nd Edition.ASM press,Washington DC,USA. 3) Murphy, F.A.,Gibss ,J.P.,Horzinek.M.C.,and Studdert,M.J.(1999).Veterinary Virology.2nd edition.Elsevier,Inc.Califonia,USA. SHAREEFNGUNGUNI6/1/2017 9
  10. 10. THANKS FOR YOUR ATTENTION SHAREEFNGUNGUNI6/1/2017 10

×