Answer: Grow mutagenized yeast on rich media. Then grow them on minimal media, as well as on minimal media containing histidine, minimal media containing leucine, and minimal media containing both histidine and leucine. Select for yeast that do not grow on minimal media, do not grow on media supplemented with only histidine or leucine, but do grow on minimal media supplemented with both histidine and leucine Reason: A genetic screen to isolate nutritional mutants in yeast needed to isolate a double mutant that cannot synthesize histidine or leucine, two nutrients essential for growth. Initially a wild type yeast strain subjected to mutagen irradiation with a UV light followed by promoting growth of mutagenized yeast on rich media & followed by its growth on minimal media, as well as on minimal media containing histidine, minimal media containing leucine. This process is essential to isolating such a mutant using minimal media containing both histidine and leucin finally yeastis going to be selected that do not grow on minimal media supplemented with either histidine or leucine finally a double mutant can be isolated of yeast that do grow on minimal media supplemented with both histidine and leucine. Solution Answer: Grow mutagenized yeast on rich media. Then grow them on minimal media, as well as on minimal media containing histidine, minimal media containing leucine, and minimal media containing both histidine and leucine. Select for yeast that do not grow on minimal media, do not grow on media supplemented with only histidine or leucine, but do grow on minimal media supplemented with both histidine and leucine Reason: A genetic screen to isolate nutritional mutants in yeast needed to isolate a double mutant that cannot synthesize histidine or leucine, two nutrients essential for growth. Initially a wild type yeast strain subjected to mutagen irradiation with a UV light followed by promoting growth of mutagenized yeast on rich media & followed by its growth on minimal media, as well as on minimal media containing histidine, minimal media containing leucine. This process is essential to isolating such a mutant using minimal media containing both histidine and leucin finally yeastis going to be selected that do not grow on minimal media supplemented with either histidine or leucine finally a double mutant can be isolated of yeast that do grow on minimal media supplemented with both histidine and leucine..

1. Answer: Grow mutagenized yeast on rich media. Then grow them on minimal media, as well as
on minimal media containing histidine, minimal media containing leucine, and minimal media
containing both histidine and leucine. Select for yeast that do not grow on minimal media, do not
grow on media supplemented with only histidine or leucine, but do grow on minimal media
supplemented with both histidine and leucine
Reason:
A genetic screen to isolate nutritional mutants in yeast needed to isolate a double mutant that
cannot synthesize histidine or leucine, two nutrients essential for growth. Initially a wild type
yeast strain subjected to mutagen irradiation with a UV light followed by promoting growth of
mutagenized yeast on rich media & followed by its growth on minimal media, as well as on
minimal media containing histidine, minimal media containing leucine. This process is essential
to isolating such a mutant using minimal media containing both histidine and leucin finally
yeastis going to be selected that do not grow on minimal media supplemented with either
histidine or leucine finally a double mutant can be isolated of yeast that do grow on minimal
media supplemented with both histidine and leucine.
Solution
Answer: Grow mutagenized yeast on rich media. Then grow them on minimal media, as well as
on minimal media containing histidine, minimal media containing leucine, and minimal media
containing both histidine and leucine. Select for yeast that do not grow on minimal media, do not
grow on media supplemented with only histidine or leucine, but do grow on minimal media
supplemented with both histidine and leucine
Reason:
A genetic screen to isolate nutritional mutants in yeast needed to isolate a double mutant that
cannot synthesize histidine or leucine, two nutrients essential for growth. Initially a wild type
yeast strain subjected to mutagen irradiation with a UV light followed by promoting growth of
mutagenized yeast on rich media & followed by its growth on minimal media, as well as on
minimal media containing histidine, minimal media containing leucine. This process is essential
to isolating such a mutant using minimal media containing both histidine and leucin finally
yeastis going to be selected that do not grow on minimal media supplemented with either
histidine or leucine finally a double mutant can be isolated of yeast that do grow on minimal
media supplemented with both histidine and leucine.