Results and figures Sarah and Waleed
PAF receptor antagonist inhibited UV-induced skin cancer induction in mice Mice without PAF antagonist developed many tumo...
Do PAF receptor antagonists inhibit cis-UCA induced immune suppression? YES! And so does the 5-HT receptor antagonist, ket...
Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103,...
Do cis-UCA and 5-HT share a receptor?
Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103,...
 
Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103,...
Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103,...
Are Cis-UCA and 5-HT structurally similar? YES Do cis-UCA and 5-HT share a receptor? Competitive Binding Studies /Calcium ...
Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103,...
Are Cis-UCA and 5-HT structurally similar? YES Do cis-UCA and 5-HT share a receptor? YES Immunoprecipitation Does blocking...
Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103,...
Sample size? n=5
Are Cis-UCA and 5-HT structurally similar? YES Do cis-UCA and 5-HT share a receptor? YES Does blocking 5-HT binding inhibi...
Conclusions <ul><li>Immunosuppressive effects of cis-UCA and UV radiation are mediated by activation of the 5-HT 2A  recep...
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Results and Figures

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Results and Figures

  1. 1. Results and figures Sarah and Waleed
  2. 2. PAF receptor antagonist inhibited UV-induced skin cancer induction in mice Mice without PAF antagonist developed many tumours Mice with PAF antagonist developed fewer tumours (P<0.001 Mann-Whitney U-test) Ulrich S. (2007); Molecular Carcinogenesis 46: 629-33
  3. 3. Do PAF receptor antagonists inhibit cis-UCA induced immune suppression? YES! And so does the 5-HT receptor antagonist, ketanserin!
  4. 4. Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17420-17425 PAF and serotonin receptor antagonists block cis-UCA- induced immune suppression * denotes P<0.001 PAF receptor antagonists
  5. 5. Do cis-UCA and 5-HT share a receptor?
  6. 6. Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17420-17425 5-HT and cis-UCA share binding sites
  7. 8. Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17420-17425 Saturation binding of cis-UCA to L-NCG-5HT 2A cells
  8. 9. Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17420-17425 cis-UCA activates intracellular calcium flux via the serotonin receptor
  9. 10. Are Cis-UCA and 5-HT structurally similar? YES Do cis-UCA and 5-HT share a receptor? Competitive Binding Studies /Calcium Mobilization Immunoprecipitation YES
  10. 11. Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17420-17425 Immunoprecipitation study
  11. 12. Are Cis-UCA and 5-HT structurally similar? YES Do cis-UCA and 5-HT share a receptor? YES Immunoprecipitation Does blocking 5-HT binding inhibits UV/cis-UCA induced immunosuppression? In vivo studies
  12. 13. Copyright ©2006 by the National Academy of Sciences Walterscheid, Jeffrey P. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17420-17425 Inhibition of cis-UCA- and UV-induced immune suppression with selective 5-HT receptor antagonists, anti-cis-UCA antibody, or anti-5-HT antibody *denotes P<0.01
  13. 14. Sample size? n=5
  14. 15. Are Cis-UCA and 5-HT structurally similar? YES Do cis-UCA and 5-HT share a receptor? YES Does blocking 5-HT binding inhibits UV/cis-UCA induced immunosuppression? YES In vivo studies
  15. 16. Conclusions <ul><li>Immunosuppressive effects of cis-UCA and UV radiation are mediated by activation of the 5-HT 2A receptor </li></ul><ul><li>Potential for using 5-HT receptor antagonists to prevent skin cancer by blocking UV-induced immune suppression, but further studies are needed. </li></ul>
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